breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 70,659 G→A W91* (TGG→TGA araC → DNA‑binding transcriptional dual regulator AraC
RA 100,946 T→C M61T (ATG→ACG)  murC → UDP‑N‑acetylmuramate‑‑L‑alanine ligase
RA 246,747 G→T S12S (TCG→TCT yafL → NlpC/P60 family protein YafL
JC JC 325,560 IS5 (+) +4 bp intergenic (‑196/+14) ykgH ← / ← betA uncharacterized protein YkgH/choline dehydrogenase
JC JC 458,790 IS186 (+) +6 bp :: Δ1 bp intergenic (+90/‑93) clpX → / → lon ATP‑dependent Clp protease ATP‑binding subunit ClpX/Lon protease
RA 480,295 2 bp→AA coding (13‑14/219 nt) hha ← hemolysin expression‑modulating protein Hha
RA 690,948 G→A V279V (GTC→GTT ybeX ← CorC‑HlyC family protein YbeX
RA 700,038 G→A H97Y (CAT→TAT)  umpH ← UMP phosphatase
RA 809,217 2 bp→AG coding (40‑41/1290 nt) bioA ← adenosylmethionine‑8‑amino‑7‑oxononanoate aminotransferase
RA 822,527 C→T E405K (GAA→AAA)  clsB ← cardiolipin synthase B
RA 883,396 C→T intergenic (‑8/‑277) ybjG ← / → mdfA undecaprenyl pyrophosphate phosphatase/multidrug efflux pump MdfA/Na(+):H(+) antiporter/K(+):H(+) antiporter
RA 968,837 G→A D158N (GAT→AAT)  lpxK → tetraacyldisaccharide 4'‑kinase
JC JC 1,034,134 IS30 (–) +2 bp coding (881‑882/1794 nt) hyaB → hydrogenase 1 large subunit
RA 1,040,126 G→T L277L (CTG→CTT appB → cytochrome bd‑II subunit 2
RA 1,061,935 G→A S46N (AGC→AAC)  torD → trimethylamine‑N‑oxide reductase‑specific chaperone
JC JC 1,082,736 IS30 (+) +2 bp coding (494‑495/1128 nt) efeO → ferrous iron transport system protein EfeO
RA 1,095,328 C→A R37L (CGT→CTT)  insE4 ← IS3 element protein InsE
RA 1,098,620 T→A V245V (GTT→GTA ghrA → glyoxylate/hydroxypyruvate reductase A
RA 1,111,727 2 bp→TT coding (865‑866/2544 nt) opgH → osmoregulated periplasmic glucans biosynthesis protein H
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,194,922 G→A T10T (ACC→ACT rluE ← 23S rRNA pseudouridine(2457) synthase
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
RA 1,196,277 C→T N385N (AAC→AAT icd → isocitrate dehydrogenase
RA 1,196,280 G→C A386A (GCG→GCC icd → isocitrate dehydrogenase
RA 1,196,283 A→G K387K (AAA→AAG icd → isocitrate dehydrogenase
RA 1,253,002 C→T intergenic (+17/+83) dhaR → / ← ycgV DNA‑binding transcriptional dual regulator DhaR/putative autotransporter adhesin YcgV
MC JC 1,299,499 Δ1,199 bp insH21 insH21
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,357,202 C→T intergenic (‑92/+221) sapA ← / ← ymjA putative periplasmic binding protein SapA/DUF2543 domain‑containing protein YmjA
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,441,038 G→A intergenic (‑254/‑20) ydbK ← / → ydbJ putative pyruvate‑flavodoxin oxidoreductase/DUF333 domain‑containing protein YdbJ
RA 1,497,385 4 bp→CAA coding (530‑533/1656 nt) opgD → glucans biosynthesis protein D
RA 1,584,092 G→A intergenic (‑133/+115) safA ← / ← ydeP two‑component system connector SafA/putative oxidoreductase YdeP
JC JC 1,590,536 IS2 (+) +5 bp intergenic (‑535/+314) ydeT ← / ← hipA fimbrial usher domain‑containing protein YdeT/serine/threonine‑protein kinase toxin HipA
RA 1,629,247 C→T I11I (ATC→ATT ydfZ → putative selenoprotein YdfZ
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,667,460 G→A Q369* (CAG→TAG)  mlc ← DNA‑binding transcriptional repressor Mlc
RA 1,682,459 A→G K101E (AAA→GAA)  rstA → DNA‑binding transcriptional regulator RstA
RA 1,748,841 C→T R240K (AGA→AAA)  ydhT ← uncharacterized protein YdhT
RA 1,759,711 T→C P271P (CCA→CCG sufS ← L‑cysteine desulfurase
RA 1,767,014 C→T G558S (GGT→AGT)  ydiJ ← putative FAD‑linked oxidoreductase YdiJ
RA 1,814,798 C→T T311I (ACC→ATC)  katE → catalase HPII
RA 1,848,300 2 bp→TT coding (376‑377/552 nt) ydjA ← putative oxidoreductase YdjA
RA 1,849,629 T→C L265L (TTA→CTA)  sppA → protease IV, a signal peptide peptidase
JC JC 1,856,485 IS1 (–) +8 bp coding (437‑444/948 nt) ydjH ← L‑glycero‑L‑galacto‑octuluronate kinase
JC JC 1,879,829 Δ1 bp :: IS186 (–) +6 bp :: Δ1 bp coding (115‑120/360 nt) yeaR ← DUF1971 domain‑containing protein YeaR
RA 1,894,839 T→C L12P (CTC→CCC)  pabB → aminodeoxychorismate synthase subunit 1
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,041,278 G→A intergenic (+160/‑97) msrQ → / → zinT membrane‑bound flavocytochrome MsrQ/metal‑binding protein ZinT
RA 2,052,885 G→A intergenic (+871/‑758) yeeJ → / → shiA inverse autotransporter adhesin/shikimate:H(+) symporter
RA 2,104,024 G→T I162I (ATC→ATA wbbK ← putative glycosyltransferase WbbK
RA 2,111,259 Δ1 bp coding (718/900 nt) rfbD ← dTDP‑4‑dehydrorhamnose reductase
RA 2,142,598 G→A L117L (CTC→CTT udk ← uridine/cytidine kinase
RA 2,155,197 G→A Q394Q (CAG→CAA mdtA → multidrug efflux pump membrane fusion protein MdtA
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,173,448 C→T intergenic (‑575/+834) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,176,844 IS4 (–) +13 bp coding (349‑361/855 nt) gatY ← tagatose‑1,6‑bisphosphate aldolase 2
RA 2,233,609 C→T Q4* (CAG→TAG)  yeiS → DUF2542 domain‑containing protein YeiS
JC JC 2,238,948 Δ1 bp :: IS186 (–) +6 bp :: Δ2 bp coding (337‑342/1521 nt) mglA ← D‑galactose/methyl‑galactoside ABC transporter ATP binding subunit
RA 2,242,247 C→T G241R (GGG→AGG)  yeiB ← DUF418 domain‑containing protein YeiB
RA 2,247,134 A→C Y467D (TAC→GAC)  lysP ← lysine:H(+) symporter
RA 2,282,149 G→A E506E (GAG→GAA radD → putative DNA repair helicase RadD
RA 2,307,733 C→T A295T (GCG→ACG)  yojI ← ABC transporter family protein/microcin J25 efflux protein
RA 2,310,878 2 bp→TT coding (656‑657/1056 nt) ftp ← FAD:protein FMN transferase
JC JC 2,375,828 IS186 (–) +4 bp coding (132‑135/963 nt) menC ← o‑succinylbenzoate synthase
RA 2,404,402 G→A S71L (TCG→TTG)  nuoB ← NADH:quinone oxidoreductase subunit B
RA 2,446,592 C→A P294P (CCG→CCT aroC ← chorismate synthase
RA 2,485,636 Δ1 bp coding (1263/3594 nt) evgS → sensor histidine kinase EvgS
RA 2,485,638 T→A L422* (TTA→TAA)  evgS → sensor histidine kinase EvgS
RA 2,522,369 G→A P37S (CCG→TCG)  xapR ← DNA‑binding transcriptional activator XapR
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,587,112 C→T A461V (GCA→GTA)  narQ → sensor histidine kinase NarQ
RA 2,668,977 C→T intergenic (‑81/‑55) hcaR ← / → hcaE DNA‑binding transcriptional dual regulator HcaR/putative 3‑phenylpropionate/cinnamate dioxygenase subunit alpha
RA 2,702,363 G→A A36V (GCC→GTC)  recO ← recombination mediator protein RecO
RA 2,804,133 C→T S211F (TCT→TTT)  nrdF → ribonucleoside‑diphosphate reductase 2 subunit beta
RA 2,814,459 Δ1 bp coding (275/516 nt) luxS ← S‑ribosylhomocysteine lyase
RA 2,814,461 C→T L91L (CTG→CTA luxS ← S‑ribosylhomocysteine lyase
RA 2,817,197 G→A R103C (CGC→TGC)  yqaB ← fructose‑1‑phosphate phosphatase YqaB
RA 2,823,288 C→T G161D (GGC→GAC)  recA ← DNA recombination/repair protein RecA
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,871,153 T→G N51T (AAC→ACC)  truD ← tRNA pseudouridine(13) synthase
RA 2,926,543 2 bp→TT coding (236‑237/1365 nt) ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 2,926,772 C→A G155G (GGC→GGA ppnN → nucleotide 5'‑monophosphate nucleosidase
RA 3,025,601 (T)5→4 coding (1251/1401 nt) xanQ → xanthine:H(+) symporter XanQ
RA 3,070,858 T→G K129N (AAA→AAC fbaA ← fructose‑bisphosphate aldolase class II
JC JC 3,169,131 IS30 (–) +2 bp coding (100‑101/483 nt) ygiV ← DNA‑binding transcriptional repressor YgiV
RA 3,207,549 Δ1 bp coding (179/606 nt) ttdB → L(+)‑tartrate dehydratase subunit beta
JC JC 3,263,422 IS30 (–) +2 bp coding (238‑239/1209 nt) tdcD ← propionate kinase
RA 3,279,276 C→G F121L (TTC→TTG kbaZ → putative tagatose‑1,6‑bisphosphate aldolase 1 chaperone
RA 3,296,481 G→A V25M (GTG→ATG)  dolP → division and outer membrane stress‑associated lipid‑binding lipoprotein
RA 3,324,965 G→T intergenic (‑54/+36) folP ← / ← ftsH dihydropteroate synthase/ATP‑dependent zinc metalloprotease FtsH
RA 3,326,537 C→A S133S (TCG→TCT ftsH ← ATP‑dependent zinc metalloprotease FtsH
RA 3,329,743 G→T D261Y (GAT→TAT)  dacB → peptidoglycan DD‑endopeptidase DacB
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,411,689 G→T E13* (GAA→TAA)  yhdJ → DNA adenine methyltransferase
RA 3,433,900 C→T L71L (CTA→TTA)  def → peptide deformylase
RA 3,439,485 C→T E9K (GAG→AAG)  yhdN ← DUF1992 domain‑containing protein YhdN
RA 3,450,236 Δ2 bp intergenic (‑3/+11) rplV ← / ← rpsS 50S ribosomal subunit protein L22/30S ribosomal subunit protein S19
MC JC 3,453,832 Δ5 bp coding (92‑96/420 nt) gspB ← putative general secretion pathway protein B
RA 3,474,424 T→G K43N (AAA→AAC rpsL ← 30S ribosomal subunit protein S12
RA 3,486,502 C→T T128I (ACT→ATT)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,511,466 C→T V293V (GTG→GTA yhfZ ← putative DNA‑binding transcriptional regulator YhfZ
RA 3,520,507 G→A P66L (CCC→CTC)  hofQ ← DNA utilization protein HofQ
RA 3,555,047 G→C V655L (GTC→CTC)  malT → DNA‑binding transcriptional activator MalT
RA 3,560,362 G→A intergenic (+496/+260) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,647,428 C→T A377V (GCG→GTG)  gor → glutathione reductase (NADPH)
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,719,429 G→A intergenic (‑385/‑49) yiaF ← / → yiaG DUF3053 domain‑containing protein YiaF/putative DNA‑binding transcriptional regulator YiaG
RA 3,725,176 T→G E48A (GAG→GCG)  glyQ ← glycine‑‑tRNA ligase subunit alpha
RA 3,729,440 2 bp→TT noncoding (105‑106/160 nt) xylZ ← small RNA XylZ
RA 3,730,560 C→T W69* (TGG→TAG)  xylA ← xylose isomerase
JC JC 3,767,095 IS30 (–) +2 bp coding (759‑760/843 nt) yibA → putative lyase containing HEAT‑repeat
RA 3,770,539 C→T V281M (GTG→ATG)  yibH ← inner membrane protein YibH
RA 3,773,173 Δ1 bp coding (893/1914 nt) mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,773,176 C→T S299F (TCT→TTT)  mtlA → mannitol‑specific PTS enzyme IICBA component
RA 3,815,883 +C coding (667/687 nt) rph ← truncated RNase PH
RA 3,925,834 C→T intergenic (‑201/+178) mnmG ← / ← mioC 5‑carboxymethylaminomethyluridine‑tRNA synthase subunit MnmG/flavoprotein MioC
RA 3,926,991 G→A L5L (CTG→TTG)  asnC ← DNA‑binding transcriptional dual regulator AsnC
RA 3,966,530 2 bp→TT coding (114‑115/1260 nt) rho → transcription termination factor Rho
JC JC 3,978,530 IS30 (+) +2 bp coding (1006‑1007/1080 nt) wecF → TDP‑N‑acetylfucosamine:lipid II N‑acetylfucosaminyltransferase
RA 4,003,502 G→T P226Q (CCG→CAG)  rarD ← putative transporter RarD
JC JC 4,117,984 IS30 (+) +2 bp coding (106‑107/846 nt) glpF ← glycerol facilitator
JC JC 4,123,617 IS2 (–) +5 bp coding (836‑840/1026 nt) cytR ← DNA‑binding transcriptional repressor CytR
RA 4,150,418 A→G intergenic (‑153/+29) eptC ← / ← ppc phosphoethanolamine transferase EptC/phosphoenolpyruvate carboxylase
RA 4,193,996 T→G D70A (GAT→GCT)  thiE ← thiamine phosphate synthase
RA 4,217,310 A→G S68G (AGC→GGC)  aceA → isocitrate lyase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,325,644 C→T S33N (AGC→AAC)  yjdN ← PF06983 family protein YjdN
RA 4,331,890 C→T L463L (CTC→CTT proP → osmolyte:H(+) symporter ProP
RA 4,437,890 C→T Q62* (CAG→TAG)  ytfI → protein YtfI
RA 4,473,900 C→T G52R (GGA→AGA)  bdcA ← c‑di‑GMP‑binding biofilm dispersal mediator protein
RA 4,475,493 T→C F19F (TTT→TTC yjgL → protein YjgL
RA 4,476,605 G→A S390N (AGC→AAC)  yjgL → protein YjgL
RA 4,489,016 C→T E350K (GAA→AAA)  yjgR ← DUF853 domain‑containing protein YjgR
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,532,409 T→G intergenic (‑758/+28) sgcX ← / ← yjhP putative endoglucanase with Zn‑dependent exopeptidase domain/putative methyltransferase YjhP
RA 4,546,601 C→T I502I (ATC→ATT fimD → type I fimbriae usher protein
RA 4,620,610 G→A L336L (CTG→CTA deoB → phosphopentomutase
RA 4,636,925 G→C R77P (CGC→CCC)  creC → sensory histidine kinase CreC
RA 4,638,240 G→A L21L (TTG→TTA creD → putative inner membrane protein CreD

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 256108 316257–315230 59123–60150 109 [0] [42] 43 [gpt]–[insF1] [gpt],frsA,crl,insB9,insA9,crl,phoE,proB,proA,thrW,ykfI,yafW,ykfH,ykfG,yafX,ykfF,ykfB,yafY,yafZ,ykfA,perR,insN,insI1,insN,eyeA,ykfC,insH1,mmuP,mmuM,afuC,insB2,insA2,yagB,yagA,yagE,yagF,yagG,yagH,xynR,argF,argL,ykgS,insB3,insA3,yagJ,yagK,yagL,yagM,ykgV,yagN,intF,yagP,paoD,paoC,paoB,paoA,yagU,ykgJ,ecpE,ecpD,ecpC,ecpB,ecpA,matA,ykgL,ykgO,ykgM,ykgR,insE1,[insF1]
* * ÷ NC_000913 1196291 1211460 15170 43 [42] [42] 48 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 3423816–3424233 3424492–3424239 7–677 43 [42] [40] 43 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2561070 (0.000)107 (0.960) 71/582 0.2 59.8% coding (131/459 nt) gpt xanthine‑guanine phsophoribosyltransferase
?NC_000913 371382 = 144 (1.300)coding (144/867 nt) mhpC 2‑hydroxy‑6‑ketonona‑2,4‑dienedioate hydrolase
* ? NC_000913 = 367291159 (1.390)82 (0.720) 59/596 0.7 36.9% coding (220/1083 nt) lacI DNA‑binding transcriptional repressor LacI
?NC_000913 1321270 = 122 (1.070)coding (115/1596 nt) trpD anthranilate synthase subunit TrpD
* ? NC_000913 = 89185886 (0.750)38 (0.330) 35/598 2.4 30.6% coding (675/723 nt) nfsA NADPH‑dependent nitro/quinone reductase NfsA
?NC_000913 = 1470516 NA (NA)noncoding (1221/1221 nt) IS30 repeat region
* ? NC_000913 = 1327716113 (0.990)141 (1.240) 87/596 0.1 55.9% coding (865/876 nt) rluB 23S rRNA pseudouridine(2605) synthase
?NC_000913 1464643 = 110 (0.970)coding (173/591 nt) paaY 2‑hydroxycyclohepta‑1,4,6‑triene‑1‑carboxyl‑CoA thioesterase
* ? NC_000913 = 1979270NA (NA)136 (1.200) 87/596 0.1 91.3% noncoding (1/768 nt) IS1 repeat region
?NC_000913 = 3582237 13 (0.110)coding (375/1179 nt) yhhZ putative endonuclease YhhZ
* ? NC_000913 4542682 =96 (0.840)95 (0.860) 63/580 0.4 53.2% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 74 (0.670)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 4542690107 (0.940)92 (0.830) 59/580 0.6 50.9% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 74 (0.670)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit