breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC JC 260,173 Δ13,782 bp IS5‑mediated proB[ykfC] 19 genes
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 635,355 G→A R8* (CGA→TGA) 
N405N (AAC→AAT
ybdM ←
ybdN ←
ParB‑like nuclease domain‑containing protein YbdM
putative PAPS reductase/DUF3440 domain‑containing protein YbdN
RA 696,582 C→T noncoding (35/75 nt) glnV ← tRNA‑Gln
RA 811,531 G→A G384D (GGT→GAT) 
V4I (GTT→ATT) 
bioF →
bioC →
8‑amino‑7‑oxononanoate synthase
malonyl‑acyl carrier protein methyltransferase
JC JC 998,256 IS3 (–) +4 bp coding (389‑392/540 nt) elfA → putative laminin‑binding fimbrial subunit
RA 1,097,494 T→A intergenic (+665/+71) ycdU → / ← serX uncharacterized protein YcdU/tRNA‑Ser
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,705,108 G→A G54R (GGG→AGG)  cnu → H‑NS‑ and StpA‑binding protein
RA 1,922,115 G→T intergenic (+99/‑6) yebV → / → yebW DUF1480 domain‑containing protein YebV/DUF1482 domain‑containing protein YebW
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC 2,173,675 (ATTCGGTTAACGCCTGG)1→2 intergenic (‑802/+607) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
RA 2,339,161 T→C D87G (GAC→GGC)  gyrA ← DNA gyrase subunit A
JC 2,406,557 (TGCGGCAGC)1→2 coding (85/939 nt) lrhA ← DNA‑binding transcriptional dual regulator LrhA
RA 2,778,018 C→T intergenic (+236/+128) ypjF → / ← ypjA toxin of the YpjF‑YfjZ toxin‑antitoxin system/adhesin‑like autotransporter YpjA
RA 2,779,776 G→A S984L (TCG→TTG)  ypjA ← adhesin‑like autotransporter YpjA
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 3,195,419 T→G E445D (GAA→GAC hldE ← fused heptose 7‑phosphate kinase/heptose 1‑phosphate adenyltransferase
JC JC 3,350,539 IS1 (+) +9 bp intergenic (‑80/‑30) elbB ← / → arcZ low activity glyoxalase ElbB/small regulatory RNA ArcZ
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 3,888,443 C→A I3I (ATC→ATA tnaC → tnaAB operon leader peptide
RA 3,906,751 T→C intergenic (‑184/+102) bglG ← / ← phoU transcriptional antiterminator BglG/negative regulator of the pho regulon
RA 4,132,697 G→A E28K (GAG→AAG)  metF → 5,10‑methylenetetrahydrofolate reductase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
JC JC 4,476,959 IS5 (+) +4 bp coding (1523‑1526/1815 nt) yjgL → protein YjgL
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,566,642 G→A S202L (TCG→TTG)  yjiN ← DUF445 domain‑containing protein YjiN

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978503–1979269 1979336 68–834 118 [1] [0] 95 insB5–insA5 insB5,insA5

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =44 (0.420)53 (0.530) 45/566 1.0 54.5% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 47 (0.470)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780544 (0.420)57 (0.570) 44/566 1.1 56.3% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 47 (0.470)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994980 (0.000)105 (1.040) 75/574 0.1 100% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 = 1300693 NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 1299499 =NA (NA)125 (1.240) 84/574 0.0 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1300694 = 0 (0.000)intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 = 19785021 (0.010)101 (1.210)
+62 bp
60/474 0.1 99.2% intergenic (‑305/+16) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
?NC_000913 = 2102943 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 1979337 =0 (0.000)94 (0.890) 68/598 0.3 100% intergenic (‑122/‑416) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 2288919 =NA (NA)37 (0.350) 31/598 2.5 21.6% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 3321591 134 (1.270)coding (23/1626 nt) yhbX putative hydrolase YhbX
* ? NC_000913 3321588 =130 (1.230)42 (0.400) 34/598 2.2 24.4% coding (26/1626 nt) yhbX putative hydrolase YhbX
?NC_000913 = 3366750 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 3583428 =NA (NA)77 (0.730) 61/598 0.5 67.5% noncoding (1/768 nt) IS1 repeat region
?NC_000913 4536380 = 37 (0.350)intergenic (‑2327/‑217) topAI/ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
* ? NC_000913 = 3584195NA (NA)77 (0.730) 53/598 0.8 67.5% noncoding (768/768 nt) IS1 repeat region
?NC_000913 = 4536387 37 (0.350)intergenic (‑2334/‑210) topAI/ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB