Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	1,097,494	T→A	intergenic (+665/+71)	ycdU → / ← serX	uncharacterized protein YcdU/tRNA‑Ser

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	1,097,494	0	T	A	89.4%	170.2 / 17.5	69	intergenic (+665/+71)	ycdU/serX	uncharacterized protein YcdU/tRNA‑Ser
Reads supporting (aligned to +/- strand):  ref base T (3/4);  new base A (52/7);  total (56/13)
Fisher's exact test for biased strand distribution p-value = 1.20e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

ATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTT  >  NC_000913/1097202‑1097789                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                          atGGCGGTTAGGGGGGGATTGACTCGCTTCGCGCGCCCTGCGGGAAGCGCGCTCACTACGTTCACGGTCTGTCCAACGTGCTGTCGCCGTTTTTCGAACCCCGGTCGGGGTTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATc                                                                                                                                                                                                                                                                                                    <  2:268396/300‑1 (MQ=11)           gggggggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑gtgt                                                                                                                                                                                                                                                                                         >  1:716728/1‑300 (MQ=17)             gggggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCAGGT‑GTGTGCAATATACGAAAAACAAGCACGTACGTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCACCAG‑gtgcgt                                                                                                                                                                                                                                                                                       >  1:634335/1‑297 (MQ=255)                gggttGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCCCTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTAATCCCCCCG‑GTGTGTGCa                                                                                                                                                                                                                                                                                    >  1:651542/4‑300 (MQ=12)                  aTTGCCTCGCGTCGCTCGCCCTGCGGGCAACCCGCTCACTGCGTTCACGGTCTTNCCAACTGACTTTCGCCATTTGTCGAACCCTGGGCGGGGCTTCTCA‑CCCCTAGGTTGGGGGCAATGGATGGGAGGAGGGACCGTAATTTTGTGCGA‑‑TCTTTTTTGGGGGTGGTGAGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCG‑GTGTGTGCAat                                                                                                                                                                                                                                                                                  <  2:623570/300‑1 (MQ=2)                         cgcttcgctCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGaa                                                                                                                                                                                                                                                                           >  1:248515/1‑300 (MQ=17)                                    cccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCAG‑GTGTGTGCAATATACGAAAAAAAAGCCCg                                                                                                                                                                                                                                                                >  1:220170/1‑300 (MQ=17)                                     ccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGt                                                                                                                                                                                                                                                               >  1:203814/1‑300 (MQ=17)                                      cTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑TTGTGTGCAATATACGAAAAAAAAGCCCGTa                                                                                                                                                                                                                                                              >  1:432975/1‑300 (MQ=17)                                              aGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGAAAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTa                                                                                                                                                                                                                                                      >  1:318250/1‑300 (MQ=17)                                                            gTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTa                                                                                                                                                                                                                                        >  1:727089/1‑300 (MQ=17)                                                            gTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAACCCGCTCACTGCGTTAACGGTCTGT‑CACACTGGCTGTCGCCAGTTGTCGCACCCCGGTCGTGGCATCTCATACGAAAG‑GTGTGTGCAATATCGGCAAAAAAAGCACGTACTTTCGTACGAGCTCTTCTTTa                                                                                                                                                                                                                                        >  2:84413/1‑300 (MQ=11)                                                             ttCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTATGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCCGT‑CCACCTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATACCCCAG‑GTGTGTGCAATATACGCAAAAAAAGACCGTACTTTCTTACGAGCTCTTATTTac                                                                                                                                                                                                                                       >  1:47067/1‑299 (MQ=11)                                                                       gTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGt                                                                                                                                                                                                                             >  1:3670/1‑300 (MQ=17)                                                                                gCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGATCTTCTTTAAATATGGCGGTGAggggggg                                                                                                                                                                                                                    >  1:684388/1‑300 (MQ=17)                                                                                gCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTATGCAATATACGAAACCAAAGCCCGTACTTTCGTATGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCCACTGGCTGTCGCCAGTTGTCGCACCCCGGTCTGGGCATCTCATCCCCCCG‑GTGTGCTCCATATACTAAAAAAAAGCCCGTACTTTCGTACGAGTTCTTCTTTAAATCTGGCGGTGAggggggg                                                                                                                                                                                                                    >  1:682777/1‑300 (MQ=11)                                                                                  ttgcGCCAGTTGTCGAACCCCGTTCGTGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGGGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAt                                                                                                                                                                                                                  <  1:503081/297‑1 (MQ=12)                                                                                             gTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCGTCTCATCCCCCAG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGAttgactcgcctc                                                                                                                                                                                                       >  1:507959/1‑290 (MQ=2)                                                                                             gTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑Cg                                                                                                                                                                                                      >  1:161777/1‑300 (MQ=37)                                                                                             gTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTATTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑Cg                                                                                                                                                                                                      >  1:857074/1‑300 (MQ=37)                                                                                                                 tctcATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTATTNCCATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTANacacac                                                                                                                                                                                  >  2:693654/1‑300 (MQ=255)                                                                                                                    cATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCTCTTCGCTCGCCCCACTAGCCGCCCTCTCACTGCGTTCCCGTTCTGT‑CCCCCTGGCTGTCACCAGTTGTATAACCCCGGTCGGGACATCTCATCCACCAA‑GTGTGTGCAATATCCGAAAAAAAGGCACGTACTTTCGGACGAGCTCTCATTTCCATCTGAAGGTGAGGAGGGGCTTCGAACCCC‑CGATATGTTGCCGtatctaccctct                                                                                                                                                                               >  2:427015/1‑291 (MQ=2)                                                                                                                        cccccGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTCAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAg                                                                                                                                                                           >  1:287539/1‑300 (MQ=255)                                                                                                                        cccccGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAg                                                                                                                                                                           >  1:24257/1‑300 (MQ=255)                                                                                                                                 gtgtgCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGCACCCC‑CGATACGTTGCAGTATACACACTCTCCAGGCGTGCTc                                                                                                                                                                   >  1:560552/1‑300 (MQ=255)                                                                                                                                   gtgCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCCGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTCTACACACTTTCCAGGCGTGCTCCt                                                                                                                                                                 >  2:323059/1‑300 (MQ=255)                                                                                                                                        atatACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGc                                                                                                                                                            >  1:550394/1‑300 (MQ=255)                                                                                                                                         tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑TCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGAAGCCTGCTCACTTCGTTCACGGTC‑GTGCCGACTGGTTGTCGTCGGTTGTTGAACCCCGTTCGGGGCATCTCATCCCCTAG‑GGGGGGGAAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGcc                                                                                                                                                           <  2:390893/300‑1 (MQ=17)                                                                                                                                         tataCGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAAGATG‑‑GCGGTTATGTGGGGATTGACTCGCTTCGCTCGCCCTGCGTGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGcc                                                                                                                                                           <  2:3667/300‑1 (MQ=255)                                                                                                                                                           ttACTTTCGTGCGAGCTCTTCTTTAAATCTG‑‑GCGGGGAGGGGGGGAGTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCaa                                                                                                                                         <  1:411992/299‑1 (MQ=255)                                                                                                                                                                              tcttTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCTGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCAACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGc                                                                                                                      >  1:776165/1‑300 (MQ=255)                                                                                                                                                                              tcttTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGTAGTATACACACTTTCAAGGCGTGCTCCTTCAGCCACTCGGACACCCCCACAAATTGTCGTTCCTGTCTTGc                                                                                                                      >  1:169012/1‑300 (MQ=255)                                                                                                                                                                              tcttTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑AGATACGTTGCCGTATACACACTTTCAAGGCGTGTTCCTTCAGCCACTCGGACACCTAAACAAATTGTCGTTCCTGTCTTGc                                                                                                                      >  2:414865/1‑300 (MQ=255)                                                                                                                                                                               cttTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATANGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt                                                                                                                     >  1:611609/1‑300 (MQ=255)                                                                                                                                                                               cttTAAATACT‑‑GCGGTGAGGTGGGTATTGACTCGCTTCGCTCGCCCTGCGGGCACCCCGCTCACTGCATTCACGGGCTGT‑CCAACCGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCGTCTCACCCCCCCN‑GTATGTACAATATACGAAAAAAAAGCCCGTACTTGCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCt                                                                                                                     <  2:198785/300‑1 (MQ=18)                                                                                                                                                                                   aaaTATG‑‑GCGGTGAGGGGGTGATTGACTCGCTTCGCTCGCCCTGCGTGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGaa                                                                                                                 <  2:316321/300‑1 (MQ=255)                                                                                                                                                                                     atatG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACg                                                                                                               >  1:137456/1‑300 (MQ=255)                                                                                                                                                                                       atactgCGGTGAGTGTGGGATTGACTCGCTTCGCGCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGCCTGTCGCCAGTTGTCGAACCCCGTTCTGGGCATCTCATCCCCCCG‑GGGTGTGCAATAGACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTGGAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACg                                                                                                               <  1:134487/295‑1 (MQ=21)                                                                                                                                                                                                 gAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCAGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAAtt                                                                                                     >  1:779753/1‑300 (MQ=255)                                                                                                                                                                                                   ggggggggTTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTTACTGTGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCANTATACGAAAAAAAAGCCCGTACTTTCNTCCGCGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCTTGCTCCTTCAGCCACCCGGACACCTCACCCAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTa                                                                                                   >  2:587471/1‑300 (MQ=21)                                                                                                                                                                                                   gggggggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGACAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCATTCAGCCACTCGGACACCTCAACAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTa                                                                                                   >  2:157921/1‑300 (MQ=255)                                                                                                                                                                                                   gggggggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTa                                                                                                   >  1:107845/1‑300 (MQ=255)                                                                                                                                                                                                   gggggggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTAGTTCCTGTCTTGCTGGAACGGGCGCTAATTTa                                                                                                   >  1:101173/1‑300 (MQ=255)                                                                                                                                                                                                        ggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGTAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCAACAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaa                                                                                              >  1:408047/1‑300 (MQ=255)                                                                                                                                                                                                        ggATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGaa                                                                                              >  1:717855/1‑300 (MQ=255)                                                                                                                                                                                                             gACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCAt                                                                                         >  1:802913/1‑300 (MQ=255)                                                                                                                                                                                                             gACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTAATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATAGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCAACAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCAt                                                                                         >  1:796496/1‑300 (MQ=255)                                                                                                                                                                                                                 ggcttggagatgtgtataagagacatgCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCG‑GTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAcc                                                                                     <  1:775324/274‑1 (MQ=21)                                                                                                                                                                                                                  gcttcgctCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCt                                                                                    >  1:515762/1‑300 (MQ=255)                                                                                                                                                                                                                             ccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTATGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAACTGAGGTcaacaa                                                                         >  2:386400/1‑300 (MQ=255)                                                                                                                                                                                                                             ccTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGACCCCC‑CGATACGTTGCCGTATCCACACTTTCCAGGCGTGCTCATTCAGCCACTCGGACACCTCACCAACGTATCGTTCCTGTCTTGCTGGAACAGGCGCTAATTTCGTGAAATCCTGACCTGAAGTcaacaa                                                                         >  2:353528/1‑300 (MQ=18)                                                                                                                                                                                                                                          cGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGACGTATACGCACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAAAAAACTTTTTGaaaaa                                                            >  2:246717/1‑300 (MQ=255)                                                                                                                                                                                                                                           gCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGACCCCCGGTCGGGGCTTCTCATCCCCCCG‑GTGTGTGCAATACACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCAGTATACACACTTTCCAGGCGTGCTCCTTCCGCCACTCGGACACCTCACCAACTTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAccccgtctcttctacacctctagcaagg                                                           >  2:761180/1‑274 (MQ=21)                                                                                                                                                                                                                                            cTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGNGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAANGGGTGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAAt                                                          >  2:792168/1‑300 (MQ=255)                                                                                                                                                                                                                                                   cGTTCACGGTCTGT‑CCAACTGGTTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCATTCAGCCACTCGGACACCTCACCAACTTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATcgcgcat                                                   >  2:139176/1‑298 (MQ=255)                                                                                                                                                                                                                                                    gTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGCACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGTTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAGCTTTTTGAAAAAATCGCGCGtt                                                  >  2:185026/1‑300 (MQ=255)                                                                                                                                                                                                                                                     ttCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGttt                                                 >  2:442693/1‑300 (MQ=255)                                                                                                                                                                                                                                                         cGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTAATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTATTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCAGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCCCCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGAACTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTc                                             >  2:227785/1‑300 (MQ=255)                                                                                                                                                                                                                                                          ggTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCGGTGTGTGCGCAATATACGAAAAAAGAGCATGTATTTTCGTACGAGCTCTTCTTTACATATGGCGGCGAGGGGGGGATTC‑ACCCCCACGATACGTTACCGTATACACCTTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAAGTGTCGTTCATGTCTTGATGGAAAGGGCGCGAGTTTAGAGAACCCACAACATGAGGTCAACAACCTTTTTGAAAACATCGCGTGTttcttc                                             >  2:346237/1‑300 (MQ=16)                                                                                                                                                                                                                                                             cTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAAc                                         >  1:826732/1‑300 (MQ=255)                                                                                                                                                                                                                                                                         gCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAAtg                              >  1:438972/1‑300 (MQ=255)                                                                                                                                                                                                                                                                           tGTCGCCAGTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GGGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAAtgtg                            <  2:550657/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                   gTTGTCGAACCCCGGTCGGGGCATCTCATCCCCCAG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTTCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAAAAAACTTTTTGCAAAAATCGCGCGCTTATTCAAACTTCAATCAATGTGTGGGTTta                    >  2:113003/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gTCGAACCCCGGTCGGGGCTTCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTTGCCCCCC‑CGATCCGTTGCAGTATACACACTTTCCAGGCGTTCTCCTTCCGCCACTCGGACACCTCCCCACATTGTCATTCCTGTCTTGCTGGACCGGGCGCTAATTTAGGGCAATCATAACCCGAGGTAAACAAACTTTTTGAAAAAATCTCGCGTTTATTCAAACTTCAATGAAGGTGTGGctttccta                 >  1:818196/1‑296 (MQ=21)                                                                                                                                                                                                                                                                                      gTCGAACCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGCTTCGAACCCC‑CGATACGTTGCCGTCTACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACATCTCCCCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTACTTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGATTTtacga                 >  2:461879/1‑297 (MQ=37)                                                                                                                                                                                                                                                                                           aCCCCGGTCGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGa            >  1:488150/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                               tggTCGGGGCGTCTCATCCCCTCG‑GTGTGGGCAAGATAAGAAAAAAAAGCCCGTACTTTCGTACGAGCTCGTCTTTAAATATGGCGGTGGGGGGGGGAGTCGAACCCC‑TGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAAGTGTCGTTCCTGTCTGGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTGTAATAAGCGAAATc        <  2:306308/299‑1 (MQ=37)                                                                                                                                                                                                                                                                                                   cGGGGCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCt    <  1:389531/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                                     gggCATCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCttt  >  1:818547/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                                                                          ATGGCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGTCCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCGGT‑GTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATG‑‑GCGGTGAGGGGGGGATTGACTCGCTTCGCTCGCCCTGCGGGCAGCCCGCTCACTGCGTTCACGGTCTGT‑CCAACTGGCTGTCGCCAGTTGTCGAACCCCGGTCGGGGCTTCTCATCCCCCCG‑GTGTGTGCAATATACGAAAAAAAAGCCCGTACTTTCGTACGAGCTCTTCTTTAAATATGGCGGTGAGGGGGGGATTCGAACCCC‑CGATACGTTGCCGTATACACACTTTCCAGGCGTGCTCCTTCAGCCACTCGGACACCTCACCAAATTGTCGTTCCTGTCTTGCTGGAACGGGCGCTAATTTAGGGAAATCATGACCTGAGGTCAACAAACTTTTTGAAAAAATCGCGCGTTTATTCAAACTTCAATCAATGTGTGGTTTTAATAAGCGAAATCTGCTTT  >  NC_000913/1097202‑1097789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑