Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	811,531	G→A	G384D (GGT→GAT)  V4I (GTT→ATT)	bioF → bioC →	8‑amino‑7‑oxononanoate synthase malonyl‑acyl carrier protein methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	811,531	0	G	A	100.0%	184.5 / NA	52	G384D (GGT→GAT)  V4I (GTT→ATT)	bioF bioC	8‑amino‑7‑oxononanoate synthase malonyl‑acyl carrier protein methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (20/32);  total (20/32)

GTGATGCACGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAG  >  NC_000913/811243‑811819                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                 gTGATGCACGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCNTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACaa                                                                                                                                                                                                                                                                                       >  2:756222/1‑300 (MQ=255) gTGATGCACGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACaa                                                                                                                                                                                                                                                                                       >  2:386994/1‑300 (MQ=255)         cGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGc                                                                                                                                                                                                                                                                               <  1:91407/300‑1 (MQ=255)         cGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGGCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGc                                                                                                                                                                                                                                                                               <  1:118385/300‑1 (MQ=255)                      tgtcggcACTCATTACGCGTTTTCGGGCCTGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCTTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGt                                                                                                                                                                                                                                                                  <  2:152828/297‑1 (MQ=255)                      tGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCATCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGNGTCACGGCGATTCGCCCGCCAACCGTNCCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGt                                                                                                                                                                                                                                                                  <  2:582638/300‑1 (MQ=255)                      tGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGt                                                                                                                                                                                                                                                                  <  1:53905/300‑1 (MQ=255)                                cATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCg                                                                                                                                                                                                                                                        >  1:816736/1‑300 (MQ=255)                                     cgcgTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGTTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCacac                                                                                                                                                                                                                                                   <  1:108163/300‑1 (MQ=255)                                     cgcgTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCacac                                                                                                                                                                                                                                                   <  1:90669/300‑1 (MQ=255)                                           ttCGGGCCGGAGGACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAg                                                                                                                                                                                                                                             <  2:805863/300‑1 (MQ=255)                                             cGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCa                                                                                                                                                                                                                                           <  1:144339/300‑1 (MQ=255)                                             cGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCCTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCa                                                                                                                                                                                                                                           <  2:825099/300‑1 (MQ=255)                                                      gTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGa                                                                                                                                                                                                                                  <  1:506556/300‑1 (MQ=255)                                                           ggATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTAc                                                                                                                                                                                                                             <  1:745467/300‑1 (MQ=255)                                                                        aCGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCACTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGc                                                                                                                                                                                                                <  1:845475/300‑1 (MQ=255)                                                                             tGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACg                                                                                                                                                                                                           <  1:221979/300‑1 (MQ=255)                                                                                 gATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCtt                                                                                                                                                                                                       >  1:752803/1‑300 (MQ=255)                                                                                      aTGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTgg                                                                                                                                                                                                  >  1:856207/1‑300 (MQ=255)                                                                                             gcCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCAGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCt                                                                                                                                                                                           >  2:161212/1‑300 (MQ=255)                                                                                                  ccAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCcac                                                                                                                                                                                      <  1:472788/300‑1 (MQ=255)                                                                                                            gcaagatgtgtataagagacagCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATa                                                                                                                                                                            >  1:104189/22‑300 (MQ=255)                                                                                                            attgTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACTGCGATTCGCCCGCGAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATa                                                                                                                                                                            <  2:804480/300‑1 (MQ=255)                                                                                                            attgTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATa                                                                                                                                                                            <  1:554514/300‑1 (MQ=255)                                                                                                             ttgTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATac                                                                                                                                                                           <  1:708978/300‑1 (MQ=255)                                                                                                                  ggTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCa                                                                                                                                                                      >  2:373108/1‑300 (MQ=255)                                                                                                                       tAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACGCCCACGTAc                                                                                                                                                                 <  1:777046/300‑1 (MQ=255)                                                                                                                       tAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTAc                                                                                                                                                                 <  1:776811/300‑1 (MQ=255)                                                                                                                           aGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGa                                                                                                                                                             <  2:414266/300‑1 (MQ=255)                                                                                                                                 gCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCggg                                                                                                                                                       >  1:216868/1‑300 (MQ=255)                                                                                                                                   gTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAAGTACTGGACGCGGGtt                                                                                                                                                     >  1:676843/1‑300 (MQ=255)                                                                                                                                        aaCTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGa                                                                                                                                                <  1:765821/300‑1 (MQ=255)                                                                                                                                         aCTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGAc                                                                                                                                               <  1:501672/300‑1 (MQ=255)                                                                                                                                                    aaCTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATg                                                                                                                                    <  1:764861/300‑1 (MQ=255)                                                                                                                                                            cAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGCTGAGCCGCCa                                                                                                                            >  2:25635/1‑300 (MQ=255)                                                                                                                                                             aGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTTGCTGGATGAGCCGCCAc                                                                                                                           <  1:43801/300‑1 (MQ=255)                                                                                                                                                                                         cccgccAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCAAGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACgg                                                                                               >  1:841063/1‑300 (MQ=255)                                                                                                                                                                                          ccgccAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGNATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGTTCGGGCAGCCGCACACTATTAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGc                                                                                              <  2:640711/300‑1 (MQ=255)                                                                                                                                                                                          ccgccAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGACATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGTTGACGGc                                                                                              <  2:42302/300‑1 (MQ=255)                                                                                                                                                                                                 ccGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAt                                                                                       <  1:711038/300‑1 (MQ=255)                                                                                                                                                                                                 ccGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGAGTAATAAACAAGCCATTGCATCGGCATTTGGTCGGGCAGCCGCACGCTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGCGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAt                                                                                       <  2:835868/300‑1 (MQ=255)                                                                                                                                                                                                  cGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAAGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGAtc                                                                                      <  1:353602/300‑1 (MQ=255)                                                                                                                                                                                                        cGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTcgc                                                                                >  1:436126/1‑300 (MQ=255)                                                                                                                                                                                                        cGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGAAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTGCTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTAACGCGCAGGTGACGGCCTTAGATCTCTcgc                                                                                >  2:513760/1‑300 (MQ=255)                                                                                                                                                                                                             gTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCATTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGNCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCa                                                                           >  2:641818/1‑300 (MQ=255)                                                                                                                                                                                                                                 acaccaaCCGCTGCGCACGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCAAGAGTGCTGACGCCTTACTGGCAATGCTTCCCAAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGCCCTGGCAGGATGAACCGCCACTGGCGGGAACGTCNAGCGCAGGTAACGGCCTTAGATCCCTCGCCGCCAATGCTTATTAAGGCaagaaa                                                       >  2:645007/6‑295 (MQ=255)                                                                                                                                                                                                                                     caaCCGCTGCGCATGAAATGCAAGATATCGATCGTCTACTTGAGGTGCTGCACGGCAACGATTAATAGACAAGCCATTGCAGCGGCACTTGGCCATGCAGCCGCACACTATAAGCAACCTCCAACTCTCAATCGCAAGAATACCAACGAAATACGTGCAACGCGTCAGTAGCCGAAAGACGCACATGGATAGCACACGGGGGATGGACATGGTCGAACGAGTCGCCACTGTCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAg                                                   <  2:439247/299‑1 (MQ=255)                                                                                                                                                                                                                                                        gCAGGACATCGACCGTCTGCTGTAGGTGCTGCGTGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGAAGCCGCACACTATGAGTAATATGCAGATCTACAGCGCCAGAGTGCTGGCGCCTTACGGGCAATGCTTCCACAGCGTAAAGACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATc                                <  2:127901/300‑1 (MQ=255)                                                                                                                                                                                                                                                                          gctgGAGGTGCTGCATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATCTCGAAt              >  2:728876/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                 gTGCTGCATGGAAACAATTAATAAACAAGCAATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCAAGAGTGCTGACGCCCTACTGGCAATGCTTCCCCAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGGTGAGCCGCCACTGGCGGGAACGTCACGCGAAGGTGACGGACTTAGATTTCTCGACGACAATGCTTGTTCAGGCACGCCAGAAGGATGCAGCAGACCATTATCTGGCGGGCGATATCGAATCCCTGcc       >  2:337492/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                    ctgcATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTACACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTATCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGACGGGAGATATCGAATCACTGCAGtt    >  2:130125/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                      gcATGGCAACGATTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAg  >  1:47181/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                                 |                                                                                                                                                                                                                                                                                                 GTGATGCACGGCGCGAAAAACTGGCGGCACTCATTACGCGTTTTCGTGCCGGAGTACAGGATTTGCCGTTTACGCTTGCTGATTCATGCAGCGCCATCCAGCCATTGATTGTCGGTGATAACAGCCGTGCGTTACAACTGGCAGAAAAACTGCGTCAGCAAGGCTGCTGGGTCACGGCGATTCGCCCGCCAACCGTACCCGCTGGTACTGCGCGACTGCGCTTAACGCTAACCGCTGCGCATGAAATGCAGGATATCGACCGTCTGCTGGAGGTGCTGCATGGCAACGGTTAATAAACAAGCCATTGCAGCGGCATTTGGTCGGGCAGCCGCACACTATGAGCAACATGCAGATCTACAGCGCCAGAGTGCTGACGCCTTACTGGCAATGCTTCCACAGCGTAAATACACCCACGTACTGGACGCGGGTTGTGGACCTGGCTGGATGAGCCGCCACTGGCGGGAACGTCACGCGCAGGTGACGGCCTTAGATCTCTCGCCGCCAATGCTTGTTCAGGCACGCCAGAAGGATGCCGCAGACCATTATCTGGCGGGAGATATCGAATCCCTGCCGTTAG  >  NC_000913/811243‑811819

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑