breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 38 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_000913339,9860GA66.7% 35.9 / 31.9 36R73Q (CGA→CAA) yahHputative uncharacterized protein YahH
*NC_000913339,9790GA61.1% 27.1 / 30.1 36V71M (GTG→ATG) yahHputative uncharacterized protein YahH
*NC_0009133,054,8940GT40.0% 78.8 / 29.5 75P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009133,054,8860GT38.7% 93.9 / 30.2 76Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_0009134,136,4670AC37.0% 64.1 / 14.9 48E120D (GAA→GACyijEcystine exporter
*NC_0009133,911,5330CA35.2% 193.9 / 17.1 125intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009132,477,2650AC34.9% 133.7 / 27.2 83L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_00091358,0650CA34.0% 157.1 / 22.0 100P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009132,990,4800CA33.8% 218.0 / 32.0 161intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_0009134,296,1910AC33.3% 17.8 / 18.1 27intergenic (+397/+245)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_000913339,9920C.31.4% 71.7 / 46.0 35coding (224/321 nt)yahHputative uncharacterized protein YahH
*NC_000913834,6740CA31.3% 146.7 / 17.0 83L535L (CTC→CTAdinGATP‑dependent DNA helicase DinG
*NC_000913847,9550GA30.6% 151.3 / 43.0 109A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009133,226,2240GT29.8% 226.4 / 11.2 121intergenic (+53/‑10)ebgC/ygjIDUF386 domain‑containing evolved beta‑D‑galactosidase subunit beta/putative transporter YgjI
*NC_00091312,1520GT29.5% 154.2 / 13.4 88intergenic (‑366/‑11)yaaI/dnaKDUF2541 domain‑containing protein YaaI/chaperone protein DnaK
*NC_0009134,427,8220CA29.3% 311.0 / 12.2 133intergenic (‑400/+257)yjfZ/ytfBDUF2686 domain‑containing protein YjfZ/cell division protein YtfB
*NC_0009133,841,6630GA28.8% 91.7 / 11.8 52A26V (GCG→GTG) nepIpurine ribonucleoside exporter
*NC_0009132,365,8400TG28.5% 193.8 / 18.5 130intergenic (‑220/‑70)ais/arnBputative lipopolysaccharide core heptose(II)‑phosphate phosphatase/UDP‑4‑amino‑4‑deoxy‑L‑arabinose aminotransferase
*NC_0009132,477,2670TC28.2% 163.5 / 12.9 85A121A (GCA→GCGdsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009131,922,8150CA28.0% 186.0 / 20.7 107G52V (GGA→GTA) pphAphosphoprotein phosphatase 1

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 2357730120 (1.280)10 (0.120) 10/518 5.3 8.9% coding (827/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357926 = 100 (1.230)intergenic (+123/+116) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 265341975 (0.800)10 (0.120) 9/548 5.9 13.3% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 62 (0.720)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 781577 =56 (0.600)8 (0.090)
+GAAG
6/590 7.6 10.9% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 77 (0.820)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 69674142 (0.450)4 (0.060) 3/442 7.7 9.4% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 46 (0.660)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 = 6965060 (0.000)5 (0.060)
+45 bp
4/508 7.8 14.6% intergenic (‑2/+36) glnX/glnV tRNA‑Gln/tRNA‑Gln
?NC_000913 697057 = 69 (0.740)noncoding (77/77 nt) metT tRNA‑Met
* ? NC_000913 = 429587073 (0.780)3 (0.040) 3/520 8.6 4.8% intergenic (+76/+566) gltP/yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
?NC_000913 4296434 = 56 (0.690)intergenic (+640/+2) gltP/yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
* ? NC_000913 = 137661559 (0.630)3 (0.030) 3/572 9.1 5.2% coding (582/789 nt) ycjR 3‑dehydro‑D‑guloside 4‑epimerase
?NC_000913 1376985 = 53 (0.590)coding (154/1056 nt) ycjS D‑glucoside 3‑dehydrogenase