Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,136,467	0	A	C	37.0%	64.1 / 14.9	48	E120D (GAA→GAC)	yijE	cystine exporter
Reads supporting (aligned to +/- strand):  ref base A (13/16);  new base C (17/0);  total (32/16)
Fisher's exact test for biased strand distribution p-value = 7.75e-05
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
Rejected as consensus: Frequency below/above cutoff threshold.

GGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGCAGCCCACCGTGTTCTGGGC  >  NC_000913/4136184‑4136763                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                         ggATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGACGTGGGc                                                                                                                                                                                                                                                                                          >  2:152541/1‑300 (MQ=255)      ttCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAatat                                                                                                                                                                                                                                                                                     <  1:650346/300‑1 (MQ=255)                     aCCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGAtt                                                                                                                                                                                                                                                                      <  1:454486/300‑1 (MQ=255)                         gTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCg                                                                                                                                                                                                                                                                  >  1:724281/1‑300 (MQ=255)                                    gCCTTCGACTTTACCGCCTTACGCTGCATTTNCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACNAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTa                                                                                                                                                                                                                                                       >  2:531503/1‑300 (MQ=255)                                     ccTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGCCGTGGGAAATGTTTAGCGATTCTGATTGCCGCTTTCGGCTTAt                                                                                                                                                                                                                                                      >  2:65567/1‑300 (MQ=255)                                                   gCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAg                                                                                                                                                                                                                                        >  1:136058/1‑300 (MQ=255)                                                   gCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCAGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTAGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAg                                                                                                                                                                                                                                        >  1:285086/1‑300 (MQ=255)                                                        aCGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGNGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGAAGTGGGCAATATTTAGAGATTCTGATTGCAGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTg                                                                                                                                                                                                                                   >  2:163307/1‑300 (MQ=255)                                                                                           tCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGc                                                                                                                                                                                                <  1:708988/300‑1 (MQ=255)                                                                                                                      gcCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTggg                                                                                                                                                                     >  1:122406/1‑300 (MQ=255)                                                                                                                                          acCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTCTTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCt                                                                                                                                                 >  1:296530/1‑300 (MQ=255)                                                                                                                                           ccTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCAGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTa                                                                                                                                                >  2:699823/1‑300 (MQ=255)                                                                                                                                                           gTTACAAACCTTCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTGGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCg                                                                                                                                <  1:455194/300‑1 (MQ=255)                                                                                                                                                           gTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCg                                                                                                                                >  2:560866/1‑300 (MQ=255)                                                                                                                                                           gTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGACGTGGGCAAGAGTTCGCGGTTCTGATTGCCGATTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAGAGTGTCGTGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGTGATTGTTGCTAAACGTCTGTATGCCCg                                                                                                                                >  2:8894/1‑300 (MQ=255)                                                                                                                                                                aaaCCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATc                                                                                                                           <  1:584301/300‑1 (MQ=255)                                                                                                                                                                                  gtCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGt                                                                                                         >  1:589452/1‑300 (MQ=255)                                                                                                                                                                                         gcAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCTCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAAc                                                                                                  <  1:583534/300‑1 (MQ=255)                                                                                                                                                                                         gcAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAAc                                                                                                  <  1:706566/300‑1 (MQ=255)                                                                                                                                                                                             tGGGCGTTGGTCAGCGTAGGTGCGTGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATcc                                                                                              <  1:598414/300‑1 (MQ=255)                                                                                                                                                                                                gCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGNGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGACGTGGGCAATATTTAGCGATTCNGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTgg                                                                                           >  2:520534/1‑300 (MQ=255)                                                                                                                                                                                                     ggTCAGCGGAGGTGCGGGGAAGGTGGCGATCNTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGACGTAGGCAATATTTCGCGATTCTGATTNCCGCTTTCGGCTTATTTTTGGTGTTGGAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTAAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCACGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGAt                                                                                      >  2:513724/1‑300 (MQ=255)                                                                                                                                                                                                          gCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAGCGCCTGAGAAGTAAGCAATATTTAGAGATTCTGATTGCCGCTTTCGAATTATGTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCAATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGCTGCTAAACGTCTGTATGCCCGTCATCCGCGCGCGGATTTATTGTCATTAACATCCTGGCAGATGCTGt                                                                                 >  2:324848/1‑300 (MQ=255)                                                                                                                                                                                                            ggAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGTGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTAt                                                                               <  1:99508/300‑1 (MQ=255)                                                                                                                                                                                                             gAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGCCGTGGGCAGTATTTAGCGATTCTGATTGCAGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATg                                                                              >  2:435947/1‑300 (MQ=255)                                                                                                                                                                                                              aGGTGCGTGGGAGGTGGCGATCCTGAGCTATACCAGGCCGTGCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATgc                                                                             <  1:346772/300‑1 (MQ=255)                                                                                                                                                                                                                             ggCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGTTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAg                                                              <  1:340135/300‑1 (MQ=255)                                                                                                                                                                                                                             ggCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAg                                                              <  1:112842/300‑1 (MQ=255)                                                                                                                                                                                                                                 aTCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGATCGCCTTCTCATTAAACAAGGTTTAGAGATTTTGATTGCCGATTTCGGCTTATTTTTGGTGTTGCAGCAGTGGCAATTCGATTTCTATTAGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTACATGTATGTATGCACGTCATCAGCGAGTGGATTTATTGTCGTTCACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGtgtg                                                          >  2:276216/1‑300 (MQ=255)                                                                                                                                                                                                                                 aTCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTTCTCCTTAGGCAATATTTAGAGATTCTGATTGCCGCTTTCGGGTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTATTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGTGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGtgtg                                                          >  2:354476/1‑300 (MQ=255)                                                                                                                                                                                                                                 aTCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTTCTCCGTAGGCAATATTTCGAGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTATTCGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGTGTGGATTTATTGTCGTTAACATCCTGGCAGGTGCTGTATGTGGAGATGGTGATGAGtgtg                                                          >  2:354600/1‑300 (MQ=255)                                                                                                                                                                                                                                 aTCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGtgtg                                                          >  1:626863/1‑300 (MQ=255)                                                                                                                                                                                                                                 aTCCTGAGCTATACCATGCCGTTCTGGGTGGNGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGACGTGGGCAATATTTCGAGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCNGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGtgtg                                                          >  2:519451/1‑300 (MQ=255)                                                                                                                                                                                                                                     tGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCGCGGTGAACGCCTGCGACGTGTGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCg                                                      <  1:376338/300‑1 (MQ=255)                                                                                                                                                                                                                                      gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGGCCGCCTGCTCATTAAGCAATATTTAGAGATTCTGATTGCCGCTTTGGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTAGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTAAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGACTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTAGTGCCGAGTGTGGTCGc                                                     >  2:310088/1‑300 (MQ=255)                                                                                                                                                                                                                                      gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGGCCGCCTGCTAAGTGGGAAATATGTAGGGGTTATGGTTGCAGATTTCGGCTTAGTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTATTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGTGTGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                     >  2:8086/1‑300 (MQ=255)                                                                                                                                                                                                                                      gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGGACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                     >  1:694371/1‑300 (MQ=255)                                                                                                                                                                                                                                      gAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGc                                                     >  1:409260/1‑300 (MQ=255)                                                                                                                                                                                                                                            taCCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACt                                               <  1:728996/300‑1 (MQ=255)                                                                                                                                                                                                                                              ccggtcngctCTGGTTGGTGATTTTCGCCGCATTGGTTCTCGGTGAACGCCTGCNACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTTGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTgg                                             <  2:614940/291‑1 (MQ=255)                                                                                                                                                                                                                                                     gTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGGCCGCCTTCTCCTTGAGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTATTAGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCACGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTCTGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCa                                      >  2:217252/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTGCGCCGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCAGTGGCAACTCGATTTCTCTTAGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCa                                      >  2:443358/1‑300 (MQ=255)                                                                                                                                                                                                                                                     gTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCa                                      >  1:655340/1‑300 (MQ=255)                                                                                                                                                                                                                                                          gggTGGTGATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTTCTCCTTGGACAATATTTCGCGATTCTGATTGCCGCTTTTGGTTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTAGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGTGGCGCTGGTGGTGAGTGTGGTCGTTTTACTGATGCCGCACCGTg                                 >  2:373581/1‑300 (MQ=255)                                                                                                                                                                                                                                                                 gATTTTCGCCGCGTTGTTTCTCGGTGACCGCCTTCTCCTTAGGCAATATTTAGAGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGa                          >  2:195651/1‑300 (MQ=255)                                                                                                                                                                                                                                                                   ttttCGCCGCGTTGTTTCTCGGTGACCGCCTTCTCCTTAAACAATATTTAGCGATTGTGATTGCGGCTTTCGGTTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTAGATGAAAAGTGCCATGCTGGCAATCCTCTACGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATCGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACt                        >  2:91385/1‑300 (MQ=255)                                                                                                                                                                                                                                                                                         tGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGCAGCCCACCGTGTTCTGGgc  <  1:614002/300‑1 (MQ=255)                                                                                                                                                                                                                                                                                            |                                                                                                                                                                                                                                                                                                         GGATTTTCATGAAGCAAGTCACCAGTTACATCGGTGCCTTCGACTTTACCGCCTTACGCTGCATTTTCGGCGCTCTCGTTTTATTCATCGTCCTTTTATTACGTGGTCGCGGAATGCGCCCGACACCGTTTAAATACACCTTAGCCATTGCCCTGTTACAAACCTGCGGGATGGTTGGTCTGGCGCAGTGGGCGTTGGTCAGCGGAGGTGCGGGGAAGGTGGCGATCCTGAGCTATACCATGCCGTTCTGGGTGGTGATTTTCGCCGCGTTGTTTCTCGGTGAACGCCTGCGACGTGGGCAATATTTCGCGATTCTGATTGCCGCTTTCGGCTTATTTTTGGTGTTGCAGCCGTGGCAACTCGATTTCTCTTCGATGAAAAGTGCCATGCTGGCAATCCTCTCCGGCGTCAGTTGGGGGGCGAGCGCGATTGTTGCTAAACGTCTGTATGCCCGTCATCCGCGCGTGGATTTATTGTCGTTAACATCCTGGCAGATGCTGTATGCGGCGCTGGTGATGAGTGTGGTCGCTTTACTGGTGCCGCAACGTGAAATTGACTGGCAGCCCACCGTGTTCTGGGC  >  NC_000913/4136184‑4136763

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑