breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 39 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009131,905,0930GC50.9% 0.5 / 305.8 166G98A (GGT→GCT) yobDDUF986 domain‑containing inner membrane protein YobD
*NC_0009131,922,8150CA34.2% 167.0 / 33.5 114G52V (GGA→GTA) pphAphosphoprotein phosphatase 1
*NC_0009132,477,2650AC34.0% 160.7 / 41.0 103L122* (TTA→TGA) dsdCDNA‑binding transcriptional dual regulator DsdC
*NC_0009132,767,4450CA32.4% 190.4 / 23.5 102intergenic (+90/‑265)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_00091358,0650CA31.2% 180.0 / 29.5 125P234P (CCC→CCAdjlAco‑chaperone protein DjlA
*NC_0009133,911,5330CA31.2% 266.3 / 13.8 138intergenic (‑8/+306)pstS/glmSphosphate ABC transporter periplasmic binding protein/L‑glutamine‑‑D‑fructose‑6‑phosphate aminotransferase
*NC_0009132,990,4800CA30.3% 274.2 / 29.6 165intergenic (‑120/‑788)yqeK/ygeGprotein YqeK/TPR repeat‑containing putative chaperone YgeG
*NC_0009134,360,9650CA29.9% 328.2 / 30.9 157E324* (GAG→TAG) cadCDNA‑binding transcriptional activator CadC
*NC_0009133,054,8940GT29.7% 157.0 / 26.0 101P184Q (CCA→CAA) ygfBPF03695 family protein YgfB
*NC_0009132,767,4490CA29.5% 242.1 / 14.3 112intergenic (+94/‑261)rnlB/yfjPantitoxin RnlB/putative GTP‑binding protein YfjP
*NC_0009134,136,4670AC29.0% 124.1 / 14.0 71E120D (GAA→GACyijEcystine exporter
*NC_0009134,427,8220CA29.0% 328.1 / 18.7 169intergenic (‑400/+257)yjfZ/ytfBDUF2686 domain‑containing protein YjfZ/cell division protein YtfB
*NC_0009131,334,9660AC28.9% 264.3 / 23.2 166intergenic (+137/‑182)cysB/ymiADNA‑binding transcriptional dual regulator CysB/uncharacterized protein YmiA
*NC_0009131,351,4630CA28.8% 104.5 / 15.2 73E100* (GAG→TAG) ycjDDUF559 domain‑containing protein YcjD
*NC_0009133,054,8860GT28.7% 192.5 / 24.5 101Q187K (CAA→AAA) ygfBPF03695 family protein YgfB
*NC_000913847,9550GA28.0% 195.7 / 35.1 125A17V (GCG→GTG) glnHL‑glutamine ABC transporter periplasmic binding protein
*NC_0009133,401,3450GT28.0% 178.0 / 17.7 118intergenic (‑258/+47)mreB/csrDdynamic cytoskeletal protein MreB/regulator of CsrB and CsrC decay
*NC_0009132,955,9880CA27.9% 257.6 / 13.7 136E6* (GAG→TAG) recBexodeoxyribonuclease V subunit RecB
*NC_00091385,6510GT27.5% 200.2 / 10.9 120E8* (GAG→TAG) ilvIacetolactate synthase/acetohydroxybutanoate synthase, catalytic subunit
*NC_000913141,8490CA27.0% 236.6 / 10.2 115P140Q (CCG→CAG) hpthypoxanthine phosphoribosyltransferase

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 128732962 (0.610)9 (0.120) 8/424 5.5 19.2% intergenic (‑1/+209) tyrV/tyrT tRNA‑Tyr/tRNA‑Tyr
?NC_000913 1287624 = 32 (0.440)intergenic (‑2/+158) tyrT/purU tRNA‑Tyr/formyltetrahydrofolate deformylase
* ? NC_000913 = 4606166100 (0.980)6 (0.080) 6/420 6.3 8.9% intergenic (‑1/+34) leuV/leuP tRNA‑Leu/tRNA‑Leu
?NC_000913 4606403 = 52 (0.720)intergenic (‑2/+266) leuQ/rsmC tRNA‑Leu/16S rRNA m(2)G1207 methyltransferase
* ? NC_000913 = 281827549 (0.480)4 (0.050) 3/440 8.3 6.7% intergenic (‑1/+198) argY/argV tRNA‑Arg/tRNA‑Arg
?NC_000913 2818551 = 76 (1.010)intergenic (‑2/+2) argV/serV tRNA‑Arg/tRNA‑Ser
* ? NC_000913 = 128708443 (0.420)4 (0.040) 4/558 9.0 8.8% noncoding (153/171 nt) rttR small RNA RttR
?NC_000913 1287557 = 43 (0.450)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 69645475 (0.730)6 (0.060) 4/576 9.2 8.0% noncoding (51/75 nt) glnX tRNA‑Gln
?NC_000913 696890 = 65 (0.660)noncoding (50/75 nt) glnU tRNA‑Gln
* ? NC_000913 = 2357719109 (1.070)4 (0.040)
+G
4/596 9.4 3.5% coding (816/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357916 = 115 (1.120)intergenic (+113/+126) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 3270072 =61 (0.600)3 (0.030)
+25 bp
3/548 9.5 4.1% intergenic (+470/+144) yhaC/rnpB uncharacterized protein YhaC/RNase P catalytic RNA component
?NC_000913 4137788 = 94 (0.920)intergenic (‑131/+144) yijF/gldA DUF1287 domain‑containing protein YijF/L‑1,2‑propanediol dehydrogenase/glycerol dehydrogenase
* ? NC_000913 = 292024895 (0.930)3 (0.030) 3/578 9.8 3.1% coding (499/1341 nt) gudX glucarate dehydratase‑related protein
?NC_000913 2920614 = 98 (0.990)coding (133/1341 nt) gudX glucarate dehydratase‑related protein