breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
JC JC 258,928 IS2 (+) +5 bp pseudogene (253‑257/331 nt) crl → RNA polymerase holoenzyme assembly factor Crl
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
JC JC 785,074 IS2 (+) +5 bp coding (240‑244/381 nt) ybgS ← PF13985 family protein YbgS
JC JC 949,487 IS5 (+) +4 bp coding (79‑82/909 nt) ycaN ← putative DNA‑binding transcriptional regulator YcaN
RA 1,076,997 C→T R629H (CGC→CAC)  putA ← fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA
RA 1,093,264 C→T A130V (GCA→GTA)  dgcT → putative diguanylate cyclase DgcT
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,196,220 C→T H366H (CAC→CAT icd → isocitrate dehydrogenase
RA 1,196,232 C→T T370T (ACC→ACT icd → isocitrate dehydrogenase
RA 1,196,245 T→C L375M (TTA→CTG)  icd → isocitrate dehydrogenase
RA 1,196,247 A→G L375M (TTA→CTG icd → isocitrate dehydrogenase
MC JC 1,299,499 Δ1,199 bp insH21 insH21
JC JC 1,305,759 IS2 (–) +5 bp coding (1006‑1010/1014 nt) oppD → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppD
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
MC JC 1,397,239 Δ10,478 bp IS5‑mediated ynaJ[dgcM] ynaJ, uspE, fnr, ogt, abgT, abgB, abgA, abgR, mcaS, smrA, [dgcM]
RA 1,574,002 C→A E401* (GAA→TAA)  pqqL ← periplasmic metalloprotease
JC JC 1,596,299 IS2 (+) +5 bp intergenic (‑3857/+2314) hipB ← / ← lsrK antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
JC JC 1,655,849 IS2 (–) +5 bp coding (42‑46/342 nt) ynfB → DUF1283 domain‑containing protein YnfB
JC JC 1,859,380 IS5 (+) +4 bp coding (847‑850/1380 nt) ydjK ← putative transporter YdjK
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,043,522 C→A noncoding (36/90 nt) serU ← tRNA‑Ser
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,317,455 IS2 (–) +5 bp coding (2418‑2422/2850 nt) rcsC ← histidine kinase RcsC
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
JC JC 2,771,973 IS2 (+) +5 bp coding (178‑182/315 nt) yfjU ← putative arsenate reductase‑like protein YfjU
RA 2,773,773 C→T F152F (TTC→TTT yfjW → uncharacterized protein YfjW
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
JC JC 2,993,579 IS5 (+) +4 bp intergenic (+109/‑56) ygeH → / → ygeI putative transcriptional regulator YgeH/protein YgeI
JC JC 3,368,586 IS2 (+) +5 bp coding (760‑764/1128 nt) yhcG → DUF1016 domain‑containing protein YhcG
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,948,012 G→T intergenic (‑45/‑74) hdfR ← / → maoP DNA‑binding transcriptional dual regulator HdfR/macrodomain Ori protein
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
JC JC 4,362,049 IS1 (+) +9 bp intergenic (‑115/+494) cadC ← / ← pheU DNA‑binding transcriptional activator CadC/tRNA‑Phe

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1196277 1211486 15210 36 [33] [34] 35 [icd]–mcrA [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA
* * ÷ NC_000913 1978495 1979270 776 115 [0] [0] 150 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423812–3424233 3424486–3424238 6–675 35 [34] [33] 35 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 1397238NA (NA)115 (1.120) 80/598 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)151 (1.480) 96/598 0.0 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL
* ? NC_000913 3720633 =NA (NA)74 (0.720) 54/598 0.6 NA noncoding (1/1443 nt) IS150 repeat region
?NC_000913 3762460 = NA (NA)coding (278/4134 nt) rhsA rhs element protein RhsA
* ? NC_000913 = 3722075NA (NA)105 (1.030) 67/598 0.2 100% noncoding (1443/1443 nt) IS150 repeat region
?NC_000913 = 3762462 0 (0.000)coding (280/4134 nt) rhsA rhs element protein RhsA