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breseq version 0.39.0
mutation predictions | marginal predictions | summary statistics | genome diff | command line log |
Predicted mutations | |||||
---|---|---|---|---|---|
evidence | position | mutation | annotation | gene | description |
MC JC | 257,908 | Δ776 bp | insB9–[crl] | insB9, insA9, [crl] | |
JC JC | 258,928 | IS2 (+) +5 bp | pseudogene (253‑257/331 nt) | crl → | RNA polymerase holoenzyme assembly factor Crl |
RA | 557,635 | A→T | L36Q (CTG→CAG) | folD ← | bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase |
JC JC | 785,074 | IS2 (+) +5 bp | coding (240‑244/381 nt) | ybgS ← | PF13985 family protein YbgS |
JC JC | 949,487 | IS5 (+) +4 bp | coding (79‑82/909 nt) | ycaN ← | putative DNA‑binding transcriptional regulator YcaN |
RA | 1,076,997 | C→T | R629H (CGC→CAC) | putA ← | fused DNA‑binding transcriptional repressor/proline dehydrogenase/1‑pyrroline‑5‑carboxylate dehydrogenase PutA |
RA | 1,093,264 | C→T | A130V (GCA→GTA) | dgcT → | putative diguanylate cyclase DgcT |
JC JC | 1,105,376 | IS2 (–) +5 bp | intergenic (+83/‑34) | csgC → / → ymdA | curlin chaperone/uncharacterized protein YmdA |
RA | 1,196,220 | C→T | H366H (CAC→CAT) | icd → | isocitrate dehydrogenase |
RA | 1,196,232 | C→T | T370T (ACC→ACT) | icd → | isocitrate dehydrogenase |
RA | 1,196,245 | T→C | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
RA | 1,196,247 | A→G | L375M (TTA→CTG) | icd → | isocitrate dehydrogenase |
MC JC | 1,299,499 | Δ1,199 bp | insH21 | insH21 | |
JC JC | 1,305,759 | IS2 (–) +5 bp | coding (1006‑1010/1014 nt) | oppD → | murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppD |
RA | 1,337,394 | A→G | S522G (AGC→GGC) | acnA → | aconitate hydratase 1 |
MC JC | 1,397,239 | Δ10,478 bp | IS5‑mediated | ynaJ–[dgcM] | ynaJ, uspE, fnr, ogt, abgT, abgB, abgA, abgR, mcaS, smrA, [dgcM] |
RA | 1,574,002 | C→A | E401* (GAA→TAA) | pqqL ← | periplasmic metalloprotease |
JC JC | 1,596,299 | IS2 (+) +5 bp | intergenic (‑3857/+2314) | hipB ← / ← lsrK | antitoxin/DNA‑binding transcriptional repressor HipB/autoinducer‑2 kinase |
RA | 1,652,331 | T→C | intergenic (+2346/+397) | ydfD → / ← ynfP | lysis protein/protein YnfP |
JC JC | 1,655,849 | IS2 (–) +5 bp | coding (42‑46/342 nt) | ynfB → | DUF1283 domain‑containing protein YnfB |
JC JC | 1,859,380 | IS5 (+) +4 bp | coding (847‑850/1380 nt) | ydjK ← | putative transporter YdjK |
RA | 2,032,423 | G→A | V219V (GTC→GTT) | yedJ ← | putative HD superfamily phosphohydrolase YedJ |
RA | 2,043,522 | C→A | noncoding (36/90 nt) | serU ← | tRNA‑Ser |
RA | 2,173,363 | Δ2 bp | intergenic (‑490/+918) | gatD ← / ← gatB | galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component |
JC JC | 2,317,455 | IS2 (–) +5 bp | coding (2418‑2422/2850 nt) | rcsC ← | histidine kinase RcsC |
JC JC | 2,406,780 | IS1 (–) +8 bp | intergenic (‑139/‑774) | lrhA ← / → alaA | DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA |
MC JC | 2,558,699 | Δ6,790 bp | intZ–[eutA] | intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA] | |
JC JC | 2,771,973 | IS2 (+) +5 bp | coding (178‑182/315 nt) | yfjU ← | putative arsenate reductase‑like protein YfjU |
RA | 2,773,773 | C→T | F152F (TTC→TTT) | yfjW → | uncharacterized protein YfjW |
RA | 2,867,455 | G→A | Q33* (CAG→TAG) | rpoS ← | RNA polymerase sigma factor RpoS |
JC JC | 2,993,579 | IS5 (+) +4 bp | intergenic (+109/‑56) | ygeH → / → ygeI | putative transcriptional regulator YgeH/protein YgeI |
JC JC | 3,368,586 | IS2 (+) +5 bp | coding (760‑764/1128 nt) | yhcG → | DUF1016 domain‑containing protein YhcG |
RA | 3,486,205 | C→A | T29K (ACG→AAG) | crp → | DNA‑binding transcriptional dual regulator CRP |
RA | 3,560,455 | +G | intergenic (+589/+167) | rtcR → / ← glpG | DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG |
RA | 3,948,012 | G→T | intergenic (‑45/‑74) | hdfR ← / → maoP | DNA‑binding transcriptional dual regulator HdfR/macrodomain Ori protein |
RA | 4,296,381 | +GC | intergenic (+587/+55) | gltP → / ← yjcO | glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO |
JC JC | 4,362,049 | IS1 (+) +9 bp | intergenic (‑115/+494) | cadC ← / ← pheU | DNA‑binding transcriptional activator CadC/tRNA‑Phe |
Unassigned missing coverage evidence | ||||||||||
---|---|---|---|---|---|---|---|---|---|---|
seq id | start | end | size | ←reads | reads→ | gene | description | |||
* | * | ÷ | NC_000913 | 1196277 | 1211486 | 15210 | 36 [33] | [34] 35 | [icd]–mcrA | [icd],C0293,ymfD,ymfE,lit,intE,xisE,ymfH,ymfI,ymfJ,ymfK,ymfT,ymfL,ymfM,ymfN,ymfR,beeE,ymfQ,ycfK,tfaP,tfaE,pinE,mcrA |
* | * | ÷ | NC_000913 | 1978495 | 1979270 | 776 | 115 [0] | [0] 150 | insB5–insA5 | insB5,insA5 |
* | * | ÷ | NC_000913 | 3423812–3424233 | 3424486–3424238 | 6–675 | 35 [34] | [33] 35 | [rrlD] | [rrlD] |
Unassigned new junction evidence | |||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|
seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
* | ? | NC_000913 | = 1397238 | NA (NA) | 115 (1.120) | 80/598 | 0.1 | 100% | noncoding (1195/1195 nt) | IS5 | repeat region |
? | NC_000913 | = 1978494 | 0 (0.000) | intergenic (‑297/+24) | flhD/insB5 | DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB | |||||
* | ? | NC_000913 | 1979271 = | 0 (0.000) | 151 (1.480) | 96/598 | 0.0 | 100% | intergenic (‑56/‑482) | insA5/uspC | IS1 family protein InsA/universal stress protein C |
? | NC_000913 | = 2102947 | NA (NA) | pseudogene (443/446 nt) | wbbL | interrupted rhamnosyltransferase WbbL | |||||
* | ? | NC_000913 | 3720633 = | NA (NA) | 74 (0.720) | 54/598 | 0.6 | NA | noncoding (1/1443 nt) | IS150 | repeat region |
? | NC_000913 | 3762460 = | NA (NA) | coding (278/4134 nt) | rhsA | rhs element protein RhsA | |||||
* | ? | NC_000913 | = 3722075 | NA (NA) | 105 (1.030) | 67/598 | 0.2 | 100% | noncoding (1443/1443 nt) | IS150 | repeat region |
? | NC_000913 | = 3762462 | 0 (0.000) | coding (280/4134 nt) | rhsA | rhs element protein RhsA |