breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 119 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009133,715,3360GT69.3% 95.6 / 69.5 75P353P (CCC→CCAbisCbiotin sulfoxide reductase
*NC_000913912,9730GT64.1% 71.1 / 83.4 78P281Q (CCG→CAG) hcpprotein S‑nitrosylase
*NC_000913339,9860GA62.8% 40.2 / 40.1 43R73Q (CGA→CAA) yahHputative uncharacterized protein YahH
*NC_0009132,392,3780GT60.7% 42.9 / 108.9 89L222M (CTG→ATG) nuoMNADH:quinone oxidoreductase subunit M
*NC_000913339,9790GA60.5% 35.4 / 43.1 43V71M (GTG→ATG) yahHputative uncharacterized protein YahH
*NC_0009134,430,0530GT59.7% 66.6 / 154.0 125G64C (GGT→TGT) cycAD‑serine/alanine/glycine/:H(+)symporter
*NC_0009133,683,2430CT59.2% 90.2 / 156.6 121A69T (GCG→ACG) dctAC4 dicarboxylate/orotate:H(+) symporter
*NC_0009131,565,2450GT58.7% 43.6 / 148.6 109R163R (CGC→CGAdosPoxygen‑sensing c‑di‑GMP phosphodiesterase DosP
*NC_0009132,136,4640CA56.9% 18.0 / 144.5 102M260I (ATG→ATTwzaouter membrane polysaccharide export protein Wza
*NC_0009132,358,1260CA56.8% 21.1 / 131.0 95T240T (ACG→ACTyfaU2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
*NC_0009131,102,6000GT56.4% 58.1 / 170.2 133A199A (GCC→GCAcsgDDNA‑binding transcriptional dual regulator CsgD
*NC_0009132,410,0250GT56.0% 7.4 / 119.8 84A443D (GCT→GAT) yfbSputative transporter YfbS
*NC_0009131,004,3550CA55.9% 14.3 / 135.6 93R137S (CGC→AGC) ycbFputative fimbrial chaperone YcbF
*NC_0009131,560,0670CA55.6% 18.7 / 168.1 117G288V (GGA→GTA) ddpBputative D,D‑dipeptide ABC transporter membrane subunit DdpB
*NC_0009134,428,1990CA55.3% 17.0 / 168.7 114Q173H (CAG→CATytfBcell division protein YtfB
*NC_000913556,3790CA55.1% 7.9 / 157.0 107G59W (GGG→TGG) ybcIPF04307 family inner membrane protein YbcI
*NC_0009132,566,1040GT55.1% ‑2.3 / 104.9 69L261I (CTT→ATT) eutAethanolamine ammonia‑lyase reactivase EutA
*NC_0009132,771,8860AC54.8% 61.9 / 217.0 156F90C (TTT→TGT) yfjUputative arsenate reductase‑like protein YfjU
*NC_0009132,136,3270CA54.7% 9.3 / 137.1 95G306V (GGC→GTC) wzaouter membrane polysaccharide export protein Wza
*NC_0009132,564,5130GT54.7% 28.1 / 191.0 139intergenic (+141/‑10)yffR/yffSuncharacterized protein YffR/uncharacterized protein YffS

Marginal new junction evidence (lowest skew 10 of 13 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 4542690161 (1.520)29 (0.280) 26/580 3.1 16.8% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 131 (1.270)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 431649340 (0.380)35 (0.340) 25/588 3.3 47.5% coding (726/1137 nt) phnM RPnTP hydrolase
?NC_000913 4316545 = 38 (0.360)coding (674/1137 nt) phnM RPnTP hydrolase
* ? NC_000913 4542682 =160 (1.510)29 (0.280) 22/580 3.8 16.8% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 132 (1.280)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 1411899 =130 (1.220)16 (0.170) 14/546 5.3 10.8% coding (50/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 = 1434958 146 (1.510)intergenic (‑552/+227) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 = 265341990 (0.850)12 (0.120) 10/548 6.6 12.5% noncoding (37/100 nt) timR putative small regulatory RNA TimR
?NC_000913 2653746 = 85 (0.870)intergenic (‑291/‑109) timR/ryfA putative small regulatory RNA TimR/small regulatory RNA RyfA
* ? NC_000913 = 2947467105 (0.990)5 (0.070) 5/432 7.4 6.7% intergenic (+4/‑30) metZ/metW tRNA‑Met/tRNA‑Met
?NC_000913 2947688 = 63 (0.820)intergenic (+5/+69) metV/amiC tRNA‑Met/N‑acetylmuramoyl‑L‑alanine amidase C
* ? NC_000913 = 128690654 (0.510)10 (0.100) 7/558 7.9 15.7% coding (198/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287557 = 57 (0.580)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 281794280 (0.750)5 (0.050) 5/558 8.9 6.1% intergenic (‑82/+117) argQ/argZ tRNA‑Arg/tRNA‑Arg
?NC_000913 2818492 = 79 (0.800)noncoding (58/77 nt) argV tRNA‑Arg
* ? NC_000913 = 2153357125 (1.180)5 (0.050) 4/576 9.7 4.4% noncoding (47/144 nt)
coding (52/60 nt)
sibA
ibsA
small RNA SibA
toxic peptide IbsA
?NC_000913 2153690 = 99 (0.970)noncoding (45/136 nt)
coding (48/57 nt)
sibB
ibsB
small RNA SibB
putative toxic peptide IbsB
* ? NC_000913 = 78101647 (0.440)4 (0.040) 3/524 9.8 5.9% intergenic (+98/‑52) lysW/valZ tRNA‑Lys/tRNA‑Val
?NC_000913 781809 = 86 (0.920)intergenic (+157/‑276) lysQ/nadA tRNA‑Lys/quinolinate synthase