Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	4,279,012	T→C	D178D (GAT→GAC)	ghxP →	guanine/hypoxanthine transporter GhxP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	4,279,012	0	T	C	97.8%	159.1 / ‑3.6	47	D178D (GAT→GAC)	ghxP	guanine/hypoxanthine transporter GhxP
Reads supporting (aligned to +/- strand):  ref base T (1/0);  new base C (23/22);  total (25/22)
Fisher's exact test for biased strand distribution p-value = 1.00e+00
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.66e-01

CAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGC  >  NC_000913/4278769‑4279253                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  cAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTcgcg                                                                                                                                                                                                                                             <  2:394911/250‑1 (MQ=255)   gCCTGGTGCTGGGGCACCATATTAGCGTGCCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGAcc                                                                                                                                                                                                                                          <  2:177190/251‑1 (MQ=255)        gTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACTGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTccc                                                                                                                                                                                                                                     <  1:91391/251‑1 (MQ=255)         tGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCg                                                                                                                                                                                                                                    >  2:67569/1‑251 (MQ=255)           cTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGt                                                                                                                                                                                                                                  >  2:209477/1‑251 (MQ=255)             ggggCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGt                                                                                                                                                                                                                                  <  1:519954/249‑1 (MQ=255)             ggggCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTg                                                                                                                                                                                                                                 <  2:440034/250‑1 (MQ=255)                gCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTa                                                                                                                                                                                                                             <  1:213419/251‑1 (MQ=255)                   aCATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGt                                                                                                                                                                                                                          <  2:8440/251‑1 (MQ=255)                     catatAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTc                                                                                                                                                                                                                         >  2:88817/5‑250 (MQ=255)                       atTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCAc                                                                                                                                                                                                                       <  2:326602/250‑1 (MQ=255)                          aGCGTACCTGTCGCGCGGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTgg                                                                                                                                                                                                                    <  2:76434/250‑1 (MQ=255)                                          tGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacg                                                                                                                                                                                                    >  1:93607‑M1/1‑240 (MQ=255)                                           gggTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCTCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgac                                                                                                                                                                                                  <  1:484704‑M1/251‑13 (MQ=255)                                             gttccgtgTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCCCGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaa                                                                                                                                                                                                <  1:441843‑M1/248‑15 (MQ=255)                                                              ggTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaa                                                                                                                                                                                       >  1:344026‑M1/1‑220 (MQ=255)                                                              ggTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaa                                                                                                                                                                                       <  2:344026‑M1/243‑24 (MQ=255)                                                                            tgtAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtg                                                                                                                                                                 >  2:233060‑M1/2‑206 (MQ=255)                                                                               aaTTTCTGCAACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAATCCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaa                                                                                                                                                               <  1:120790‑M1/250‑48 (MQ=255)                                                                                aTTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatc                                                                                                                                                             <  2:111996‑M1/251‑50 (MQ=255)                                                                                aTTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatc                                                                                                                                                             >  1:270650‑M1/1‑202 (MQ=255)                                                                                   tCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgt                                                                                                                                                           <  2:93607‑M1/250‑52 (MQ=255)                                                                                         aCGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccctacccaaaaggtagtaaatcgtttgcttt                                                                                                                                                    >  2:107561‑M1/1‑193 (MQ=255)                                                                                            ggTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgctttta                                                                                                                                                  >  1:200070‑M1/1‑190 (MQ=255)                                                                                                   gtacctggaTTTTGCGCAACTTGCCTCACGGCGTGGCGCACGGGACGGGGCTTGGTATCGGTCTGTTCCTGCTGCTCCTTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCataactaatgacaacaggaaaaaaaaccatccccaaacgggagcgcatcgttggcttttaattggggg                                                                                                                                          >  2:235146‑M1/5‑183 (MQ=255)                                                                                                   gTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactgggg                                                                                                                                           >  1:98521‑M1/1‑183 (MQ=255)                                                                                                     aGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAGCCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggat                                                                                                                                         <  2:51153‑M1/250‑70 (MQ=255)                                                                                                     aGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggatt                                                                                                                                        <  1:127588‑M1/251‑71 (MQ=255)                                                                                                     aGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggatt                                                                                                                                        >  1:268296‑M1/1‑181 (MQ=255)                                                                                                      gCTGGATTTTGCGCAACTTGCCTCACGGTGCGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGGTCATTGCCGATAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCTGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaacaaaccatac                                                                                                                                                                              >  1:514659‑M1/1‑180 (MQ=255)                                                                                                       cTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGCGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgt                                                                                                                                      >  1:533741‑M1/1‑179 (MQ=255)                                                                                                         ggATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgtt                                                                                                                                     <  1:133021‑M1/250‑74 (MQ=255)                                                                                                                        tGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGTGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcg                                                                                                                       <  2:70837‑M1/249‑88 (MQ=255)                                                                                                                             cACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTGCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgtttt                                                                                                                <  2:489495‑M1/251‑95 (MQ=255)                                                                                                                              aCGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcctttaactggggattgttcagggccggaatggccacagct                                                                                                               >  1:107678‑M1/1‑156 (MQ=255)                                                                                                                                          acggcacggGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGAATTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagact                                                                                                   >  1:557970‑M1/1‑144 (MQ=255)                                                                                                                                                       tGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggatagttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggaga                                                                                       >  2:75944‑M1/1‑131 (MQ=255)                                                                                                                                                             cGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgt                                                                                 <  2:233430‑M1/250‑126 (MQ=255)                                                                                                                                                                                                     cTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagc                                         >  1:110666‑M1/1‑85 (MQ=255)                                                                                                                                                                                                                aaaCCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaacgcccctggtataggggggggcgaaggtagcattatttccgtt                             >  2:393147‑M1/1‑74 (MQ=255)                                                                                                                                                                                                                    ccGCTTGATGTTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGGGTTTTTGTCCCTGGTAGGTCatccctaacgacaacaggaaaaaaaaccatccccaaaaggtagtgactcgttggtttttaactggggattgttcgggtggctttgcggtgttttgaatgttaagcctgtgggagggagaatctggggcaggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccct                         <  1:144350‑M1/251‑182 (MQ=255)                                                                                                                                                                                                                      gCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGgcg                       >  2:528623/1‑251 (MQ=255)                                                                                                                                                                                                                      gCTTGATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccctct                       >  1:219671‑M1/1‑68 (MQ=255)                                                                                                                                                                                                                          gATGGTCTGCCCGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggggaagatagcattattttctctccctcttctt                   >  2:457452‑M1/1‑64 (MQ=255)                                                                                                                                                                                                                                    ccGTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgataacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccctcttcttcgctgtacc          >  1:526503‑M1/1‑54 (MQ=255)                                                                                                                                                                                                                                      gTTGCGCTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcattatttcctctccctcttcttcgctgtacc          >  1:34099‑M1/1‑52 (MQ=255)                                                                                                                                                                                                                                           gcTGGGTGACTTCGCGACCTTCCCGGTGGTTATGTCACTGGTAGGTCatacctaacgacaacaggaaaaaaaaccatacccaaaaggtagtgaatcgtttgcttttaactggggattgttcaggtggcgttgcggtgttttgaatgttaagactgtgggagggagaatctgtggcaggaaccgcctctggtatagggggaggcgaagatagcgttatttcctctccctcttcttcgctgtaccagataacc  >  2:126119‑M1/1‑47 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                  CAGCCTGGTGCTGGGGCAACATATTAGCGTACCTGTCGCGCTGGGTGCCGTGTTCCTGATGGGTGTGCTGTTTACGGTAATTTCTGCCACGGGTATCCGTAGCTGGATTTTGCGCAACTTGCCTCACGGTGTGGCGCACGGCACGGGGATTGGTATCGGTCTGTTCCTGCTGCTCATTGCCGCTAACGGTGTCGGTCTGGTGATTAAAAACCCGCTTGATGGTCTGCCCGTTGCGCTGGGTGATTTCGCGACCTTCCCGGTGATTATGTCACTGGTAGGTCTGGCGGTGATCATCGGCCTGGAAAAACTGAAAGTCCCTGGTGGCATTCTGCTGACCATTATCGGTATCTCAATTGTCGGTTTGATCTTCGATCCTAACGTCCATTTCTCCGGCGTTTTCGCCATGCCTTCATTGAGCGATGAAAACGGCAATTCACTGATTGGCAGCCTGGACATTATGGGCGCGCTGAATCCTGTAGTCCTGC  >  NC_000913/4278769‑4279253

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑