Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NC_000913	499,060	T→G	D305A (GAC→GCC)	aes ←	acetylesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NC_000913	499,060	0	T	G	100.0%	140.0 / NA	39	D305A (GAC→GCC)	aes	acetylesterase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (21/18);  total (21/18)

AAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGACGGCTGTCATCCAGCAGCGGATCGAACTCCGCCCCGGCAATAAAACAGGGCGGAACTTCGCGAGTGAGATCATTATTAAACAGACAGTAATACGGCGACTCGCGGTCCGCGTCGTTGCTTAAATATGCCTCTTCGTAC  >  NC_000913/498823‑499305                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      aaaGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCCCGCCGGAACATATCGAAATGAAGGCTAATCTTGTTGGCGCGTAACGCTAAAGCTGAGCGGGAAAGAAATGAGCGCCGGCGCGAAGAGCCTcggcggggggttttat                                                                                                                                                                                                                                          >  1:467789/1‑241 (MQ=255)  aaGGCGTAGGTCGTATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTtcatc                                                                                                                                                                                                                                         <  2:393961/251‑1 (MQ=255)        tAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGGATCGCTAAAGCTGAGCGGGAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGt                                                                                                                                                                                                                                   >  1:186343/1‑251 (MQ=255)         aGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGt                                                                                                                                                                                                                                   <  1:455562/250‑1 (MQ=255)             cATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtag                                                                                                                                                                                                                               <  1:413182‑M1/250‑4 (MQ=255)                                   gCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGttt                                                                                                                                                                                                                                              <  2:124693/213‑1 (MQ=255)                                       tggtgcggaTTGTCTGGAGACGCTGGAAGAGATTGCCGGGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATACTCCGGCGCGTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTTAGCGGTAAAGAGCTGAGCGCCGTCCCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctg                                                                                                                                                                                                     <  1:32027‑M1/247‑30 (MQ=255)                                        gctgcGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgat                                                                                                                                                                                                   <  1:376454‑M1/251‑32 (MQ=255)                                                  gTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgctt                                                                                                                                                                                          <  1:525670‑M1/250‑41 (MQ=255)                                                                                aaaaCCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGGTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcggcttatcaggcctacaaccggtgcc                                                                                                                                                           >  1:15290‑M1/1‑180 (MQ=255)                                                                                               tCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaatt                                                                                                                                              <  1:28865‑M1/249‑85 (MQ=255)                                                                                                              ggAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgat                                                                                                                             <  1:30797‑M1/251‑102 (MQ=255)                                                                                                                    aaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatg                                                                                                                       <  2:467789‑M1/251‑108 (MQ=255)                                                                                                                    aaTATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatg                                                                                                                       <  2:161492‑M1/251‑108 (MQ=255)                                                                                                                          aaTATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgac                                                                                                                 <  1:251138‑M1/251‑114 (MQ=255)                                                                                                                                  ccGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatctt                                                                                                          >  2:104396‑M1/1‑130 (MQ=255)                                                                                                                                   cGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAAACTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttat                                                                                                        <  2:302286‑M1/251‑123 (MQ=255)                                                                                                                                    ggCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatc                                                                                                       >  1:500066‑M1/1‑128 (MQ=255)                                                                                                                                      cgcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatc                                                                                                       >  1:239044‑M1/1‑126 (MQ=255)                                                                                                                                       gcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcagg                                                                                                    >  2:93023‑M1/1‑125 (MQ=255)                                                                                                                                       gcTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGT‑AAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGGTTTCATCATCCGTGgaggcggaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgccttatgcgatgcttgacgcatctttttcggc                                                                                                   >  1:329960‑M1/1‑124 (MQ=255)                                                                                                                                             tGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctac                                                                                               >  1:298980‑M1/1‑119 (MQ=255)                                                                                                                                              gCCACGCCGGAACATCTCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAACGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGAATTCATCATCCGTGtcgtcgtagacattagacatttgtgcctgatgcgccgcttgacgcgtcttatcaggcctacaacccatgccgcatcacgcaattggtggacaatgcctgatgcgatgcttgacgcatcttatcaggcctaca                                                                                              >  2:392789‑M1/1‑118 (MQ=255)                                                                                                                                                       gAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccg                                                                                    >  1:250562‑M1/1‑109 (MQ=255)                                                                                                                                                             atCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcg                                                                               <  2:357854‑M1/250‑148 (MQ=255)                                                                                                                                                                     gatgTCTACTCTTGTGGCCGCGTATCGCGAAAGCTGAGCGGTAAAGAACTTAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTGTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtctgatcaggcctacaaccggtgccgcatccgtcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggccgacaatgggtaccggatcggtaggccgn                                                                      <  2:250338‑M1/251‑157 (MQ=255)                                                                                                                                                                     gatgGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgctgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccgg                                                                      >  1:560370‑M1/1‑95 (MQ=255)                                                                                                                                                                        ggCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggat                                                                    >  1:231264‑M1/1‑92 (MQ=255)                                                                                                                                                                          cTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggata                                                                   >  1:551046‑M1/1‑90 (MQ=255)                                                                                                                                                                           tAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataagg                                                                >  2:512890‑M1/1‑89 (MQ=255)                                                                                                                                                                                     gCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacacccggtcccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgcc                                                      >  2:385339‑M1/1‑79 (MQ=255)                                                                                                                                                                                            aTCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccg                                               <  1:175560‑M1/251‑180 (MQ=255)                                                                                                                                                                                            aTCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatcc                                                <  1:468992‑M1/250‑179 (MQ=255)                                                                                                                                                                                                 tAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggca                                            >  2:49147‑M1/1‑67 (MQ=255)                                                                                                                                                                                                               aaaGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagt                            <  2:239044‑M1/251‑199 (MQ=255)                                                                                                                                                                                                                 aGAACTGAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaacggatcggtaggccggataaggcgtttacgccgcatccggcaagactaaagcaccagtt                           >  2:73866‑M1/1‑51 (MQ=255)                                                                                                                                                                                                                       gAGCGCCGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccga                     >  2:38552‑M1/1‑45 (MQ=255)                                                                                                                                                                                                                             cGTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaacttact              >  2:408274‑M1/1‑39 (MQ=255)                                                                                                                                                                                                                              gTCGCGAAGAGCCTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggcgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaacttactc             >  2:44597‑M1/1‑38 (MQ=255)                                                                                                                                                                                                                                         ccTCGGCGGCGGTTTTCATCATCCGTGtagtcgtaggcattagacatttgtgcctgatgcgacgcttgacgcgtcttatcaggcctacaaccggtgccgcatccggcaattggtgcacaatgcctgatgcgatgcttgacgcatcttatcaggcctacaatgggtaccggatcggtaggccggataaggcgtttacgccgcatccggcaagaatagagcaccagttaaccgaacttactctgcgcccaaat  >  1:376366‑M1/1‑27 (MQ=255)                                                                                                                                                                                                                                              |                                                                                                                                                                                                                                                      AAAGGCGTAGGTCATATTCAGGTGATGTGCCCGGGCTTTGCTGCGGATTGTCTGGAGACGCTGGAAGAGATTGCCGAGCAAAACCGTGAGGTCTTCCTCGGTGCCGGCGGGAAAAAATATGAATATATTCCGGCGCTTAATGCCACGCCGGAACATATCGAAATGATGGCTAATCTTGTTGCCGCGTATCGCTAAAGCTGAGCGGTAAAGAACTGAGCGCCGTCGCGAAGAGCCTCGTCGGCGGTTTTCATCATCCGTGAATAATGCAAAAAGGCGTGCAGCGTGCCTGGGTAGAGTTTGAACTCACAGGGCTGCTGATGCGCCGCTAACGTCTGGTAAAGCAGACGGCTGTCATCCAGCAGCGGATCGAACTCCGCCCCGGCAATAAAACAGGGCGGAACTTCGCGAGTGAGATCATTATTAAACAGACAGTAATACGGCGACTCGCGGTCCGCGTCGTTGCTTAAATATGCCTCTTCGTAC  >  NC_000913/498823‑499305

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑