Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	293,207	IS2 (–) +5 bp	intergenic (‑259/+9)	yagK ← / ← yagL	uncharacterized protein YagK/resolvase‑like catalytic domain‑containing protein YagL

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	293207 =	1 (0.020)	44 (0.730)	41/480	0.4	97.7%	intergenic (‑259/+13)	yagK/yagL	uncharacterized protein YagK/resolvase‑like catalytic domain‑containing protein YagL
	?	NC_000913	381260 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 293211	2 (0.030)	57 (0.950)	52/480	0.2	96.5%	intergenic (‑263/+9)	yagK/yagL	uncharacterized protein YagK/resolvase‑like catalytic domain‑containing protein YagL
	?	NC_000913	= 382590	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

CGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGT  >  NC_000913/381017‑381500                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 cGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTg                                                                                                                                                                                                                                            <  1:394183/250‑1 (MQ=255)               atcaTTCCCTCTGGAGTCCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTCGTACGCAATTGAGCGCATAAATATGCCTGGTTTGCAACTACCGAAACGGCAATAAAACATTGCGGACCAATCGTTGCATCCGCTTCAATAACCGAATTTTTAATCACGGTGGAAAAACCAATTTTTACGACATTGCTGATTATTATCTCTGGACGAA‑ACACAGCATAATTACTAGCCCGGCCCCCTGCATCTcc                                                                                                                                                                                                                             >  2:314281/4‑251 (MQ=255)                 aatacactctGGAGTTAGAACAGACTCGGGCTGTTACTCCAGACGATGATTACTGGTAAGTACTTGTGCGCCTAAAGATTCCTGGGTTGCAACTCCCGAGTCGGCAATAAAACATTGCGGACCAATAGTTGCGTCCGCTTCAATAACCGCAGTTTTAAGCTCGGTGGCAAAACCAATTTTTACGCCAGTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAAGTTCGAGACTGGCCCCCTGAAtctctag                                                                                                                                                                                                                           <  2:539997/245‑4 (MQ=255)                     cccTCTGGAGTTCGAACAGACACGGGCTGGTCATACAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTGCTAGACTGGCCCCCTGAATCTCCGGACaa                                                                                                                                                                                                                       <  1:550530/251‑1 (MQ=255)                              gTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCa                                                                                                                                                                                                              <  1:23127/251‑1 (MQ=255)                                  ggaCAGACACGGGCTGTTCATCCAGACAATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACAGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGCCGAATAAAC‑GCATAATTACTAGACTGGCCCCCAGCATATCCAGACAACCCatatacctt                                                                                                                                                                                                           >  2:341109/3‑245 (MQ=255)                                        acacGGGCTGTTCAACAAGAAGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTTGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTGTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAg                                                                                                                                                                                                    <  2:467552/251‑1 (MQ=255)                                                tGTTCATTCAGACGATGATTACTAGTACGTTCTGCTGCGCCTAAATATGCCTGGTTTGCAACGACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGt                                                                                                                                                                                             <  2:569253/250‑1 (MQ=255)                                                 gTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCt                                                                                                                                                                                           <  1:645669/251‑1 (MQ=255)                                                           acgataactACTGGTACGTAATTGCGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCACTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGCTTATTGTGCCAGGACGAATAAAC‑ACATACTTACTAGCCTGGCCCCCTGAATCACCAGACAACCAATATCCCTTAAATAAGGTATAGTCTTAATCCTAGt                                                                                                                                                                                 >  2:637484/9‑251 (MQ=255)                                                              atgataacttggacggccttatacgcCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACAAATCGTTGCTTCCGCTTCAATAACCGCATTTTTACTCACGGTGTCCAAACCATTTTTTACGCCATTGCTGATTATGGTGCACGTACGAATAAAC‑CCATAATTACTAGACTGGCACCCTGAATCTCCAGACAACCAATATCACTTAAATAATGGAACGTCTTAATACTAGtttt                                                                                                                                                                              >  2:378430/24‑251 (MQ=255)                                                                 atTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATCGTCTTAATACTAGgtttt                                                                                                                                                                             >  1:366631/1‑249 (MQ=255)                                                                              acTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTAAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACAAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAtggg                                                                                                                                                               >  2:671089/1‑247 (MQ=255)                                                                                  gtgCGCCTAAATATGCCTGGTTTTCAACAACCGAGTCGGCAATAAAACATTGCGGAACAATCGTTGCTTCCGCTTCAATTACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAAc                                                                                                                                                          <  2:498386/251‑1 (MQ=255)                                                                                    gcgcCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAAc                                                                                                                                                          >  1:614233/1‑249 (MQ=255)                                                                                                 ccTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCt                                                                                                                                           >  1:343911/1‑251 (MQ=255)                                                                                                        tGCAACTACCGAGTCGGCCATAAAACCTTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATATTTAATCTCGGTGGCAAAACAAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GAATACTTACTCGACTGGCCCCCTGAATCTCCAGACAACCACTCTCACTTAAATACGTGATAGTATTAATACTAGttt                                                                                                                                                                               >  2:299189/1‑208 (MQ=255)                                                                                                        tGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTTGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGttt                                                                                                                                                                               <  1:299189/208‑1 (MQ=255)                                                                                                         gCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGcc                                                                                                                                    <  1:531491/250‑1 (MQ=255)                                                                                                               aCCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGaa                                                                                                                              >  1:115738/1‑250 (MQ=255)                                                                                                                       tgCAATAAAACATTGCAGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCATGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAACTAAGTGATAGACTTAATACTAGTTTTTAGCCTAGTCATTGGAGAACAGATGATTGATGTCATAGGGCTGGAGaacgacagacg                                                                                                                     >  2:133360/2‑242 (MQ=25)                                                                                                                       ggCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGCTTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTTATGTCTTAGGGCCGGAGAACCGCatgcg                                                                                                                     >  2:255509/1‑247 (MQ=255)                                                                                                                              aaaCATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGCGAACAGATGATTGATGTCTTAGGGCCGGTGAAACGCAGACGGCGTAc                                                                                                               >  2:243253/1‑250 (MQ=255)                                                                                                                              aaaCATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTAcc                                                                                                              >  1:104663/1‑251 (MQ=255)                                                                                                                                 cATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCg                                                                                                                                   >  1:654047/1‑227 (MQ=255)                                                                                                                                 cATTGCGGACCAAACGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTGTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCg                                                                                                                                   <  2:654047/227‑1 (MQ=255)                                                                                                                                                     ttCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGt                                                                                       >  1:538157/1‑251 (MQ=255)                                                                                                                                                                      cATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAAc                                                                      <  1:243253/251‑1 (MQ=255)                                                                                                                                                                                  cGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATg                                                              <  1:65088/247‑1 (MQ=255)                                                                                                                                                                                  cGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGACAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACCGGAAAAGATGGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGaagg                                                          >  2:572978/1‑248 (MQ=255)                                                                                                                                                                                      ggCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAACGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGtctc                                                      >  2:225386/1‑251 (MQ=255)                                                                                                                                                                                          aaaCAAATTTTAACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATATTTACTAGCCTGGCCCCCTGAATCTCCAGACAACAAAAATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGTGAACAGATGTTTGATGTCTTAGGACCGGAGAAACGCAGCCGTCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTAACCCGGGGAGGACGGtcgtcctc                                                  >  2:83874/1‑245 (MQ=17)                                                                                                                                                                                                aTTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGcc                                            >  1:56283/1‑251 (MQ=255)                                                                                                                                                                                                aTTTTTACGCCAGTGCTGCATATGGTGCCAGGACGAATAAAC‑GCATAATTACAAGACTGGCCCCCTGAATCGCCAGACAACCAATATCACTTAAAGAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCCATTGCTCAGCTGAGCGTTGAACCGGGGATGACGGGCTCCCTCGTTGcc                                            <  2:434073/251‑1 (MQ=14)                                                                                                                                                                                                       cGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTgcac                                                                      >  2:279306/1‑215 (MQ=255)                                                                                                                                                                                                       cGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAAc                                                                      <  1:279306/218‑1 (MQ=255)                                                                                                                                                                                                         ccATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACAt                                   >  1:129027/1‑251 (MQ=255)                                                                                                                                                                                                                            cAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGt                <  1:479047/251‑1 (MQ=39)                                                                                                                                                                                                                                aCGAATAAAC‑GCATAATTACTAGCCTGGCCCCCTGAATCTCCAGACAAACAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGATCATCAGAGCTTTGAACCGGGGATGACGGACTCCCACGTTGCCCGACAACCTGGCGTAGCATCCAGCCAGTTAtt            >  2:130279/1‑251 (MQ=11)                                                                                                                                                                                                                                    aTAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTtctc        >  1:597778/1‑251 (MQ=35)                                                                                                                                                                                                                                            cATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGt  >  1:339919/1‑250 (MQ=32)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 CGCATCCGGTAGCGATAATTCCCTCTGGAGTTCGAACAGACACGGGCTGTTCATCCAGACGATGATTACTGGTACGTACTTGTGCGCCTAAATATGCCTGGTTTGCAACTACCGAGTCGGCAATAAAACATTGCGGACCAATCGTTGCTTCCGCTTCAATAACCGCATTTTTAATCTCGGTGGCAAAACCAATTTTTACGCCATTGCTGATTATTGTGCCAGGACGAATAAAC‑GCATAATTACTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGT  >  NC_000913/381017‑381500

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.