Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,252,952	IS2 (–) +5 bp	coding (42‑46/357 nt)	yhaI →	putative inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	34 (0.930)	32/488	0.2	97.1%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	3252952 =	1 (0.030)					coding (42/357 nt)	yhaI	putative inner membrane protein
*	?	NC_000913	= 3187426	NA (NA)	23 (0.630)	22/488	0.6	95.8%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	= 3252956	1 (0.030)					coding (46/357 nt)	yhaI	putative inner membrane protein

GGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAGG  >  NC_000913/3187183‑3187671                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      ggAGTCCGGCGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCAAGTTatc                                                                                                                                                                                                                                                 >  1:352287/1‑250 (MQ=14)        ggAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTgg                                                                                                                                                                                                                                         >  1:352098/1‑251 (MQ=35)                   gAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAg                                                                                                                                                                                                                              <  1:55548/251‑1 (MQ=40)                       aGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCtt                                                                                                                                                                                                                           <  1:167621/250‑1 (MQ=255)                            agagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTTATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTg                                                                                                                                                                                                                                          <  2:327666/230‑1 (MQ=25)                            agagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTg                                                                                                                                                                                                                                          >  1:327666/1‑230 (MQ=35)                                      gAAAACGATAAAGCGTGACTACAGCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAAGGGCATCCGCATAGTGCGCTGGGTTAGCGCGCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTcaa                                                                                                                                                                                                           <  1:229381/251‑1 (MQ=255)                                         aaCGATAAAGCGTGACTACATCAGTATCATGCCCACACCAGACGGGTTAACGGCAGCAAAGAAACTTGCAGAGGCGTTCGAGCATTATAACGAATGGAATCCGAATAGTGCGCTGGGTTATCGATCGCCACGGGAATATCTGCGGCAGCGGGCTTGAAATGGGTTAAGTGCTCACAAATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCTTCAGCTTATGCCGTTGAATTtaaca                                                                                                                                                                                                         >  2:22201/1‑250 (MQ=14)                                           cGATAAAGCGTGACGTCATCAGTATCATGCCCAAACCAGACTGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATTGCATCCGCATAGTGCGCTGGTTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACaaaa                                                                                                                                                                                                      <  2:294806/251‑2 (MQ=255)                                             aTAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGAt                                                                                                                                                                                                    <  1:126414/251‑1 (MQ=255)                                                          acatcattttcctgcctaagccAGACGGGTTAACGGCAGCAAAGAACCTTGAAGAGGCGTTTGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGGTAGCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGcc                                                                                                                                                                                        <  2:15607/230‑1 (MQ=18)                                                           cATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGa                                                                                                                                                                                      <  1:203154/251‑1 (MQ=255)                                                             acAGTTTCATGGCCAAAACAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCTCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCGTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAg                                                                                                                                                                                    <  1:172846/250‑1 (MQ=255)                                                              cAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGa                                                                                                                                                                                      >  1:398232/1‑248 (MQ=255)                                                                gTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATc                                                                                                                                                                                 <  1:110334/251‑1 (MQ=255)                                                                 aaTCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATAGCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGGTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCg                                                                                                                                                                                <  1:436056/250‑1 (MQ=255)                                                                     aggCCCAAACCAGGCGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGATCATTATAACGAATGGCAGCCGCATAGTGCGCTGGATTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTGTTAATGTGTTAATTGATAACAGATTTCTGGAAATATAGGGGCAAATCCATTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGaa                                                                                                                                                                                     <  2:237678/240‑1 (MQ=18)                                                                     aTGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGaa                                                                                                                                                                                     >  1:237678/1‑242 (MQ=255)                                                                        cccAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAAc                                                                                                                                                                         <  2:416573/251‑1 (MQ=255)                                                                                aGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCt                                                                                                                                                                 >  1:286411/1‑251 (MQ=255)                                                                                          aCGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAAttt                                                                                                                                                       <  1:309994/251‑1 (MQ=255)                                                                                            gccagcaAAGAACCTTGCAGAGGCGTTCGAGCCTTATAACGAAGGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGACTATCTGCGTCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGTAGATATAGTGGCAAATCCAGATATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAAAAAAGATTTAATCGCAGCCGCAGATGGTGAAAACGTTAACCTTTACCAATTTg                                                                                                                                                      >  2:155685/3‑250 (MQ=18)                                                                                                            gCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGACGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCAAAtt                                                                                                                                      >  2:420237/1‑250 (MQ=255)                                                                                                                                  aCGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGACGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTaa                                                                                                               <  1:420237/251‑1 (MQ=255)                                                                                                                                          catcctcagagggctgtGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATACAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAAATCAACAAAGATTTAATCGAAGCCGAAGATCGTTAAAACGTTAACCTTTCCCAACTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTg                                                                                                       <  2:286411/235‑1 (MQ=37)                                                                                                                                                 aTAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAAGAGATGTCTGGAAATATAGGTGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATtaata                                                                                                <  1:42528/251‑1 (MQ=255)                                                                                                                                                                                           aGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGCCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGCAACTGATGGACAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCCATGGGTATTa                                                       >  1:197601/1‑250 (MQ=255)                                                                                                                                                                                             cGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGaa                                                                                                                                                                                     >  1:419938/1‑122 (MQ=255)                                                                                                                                                                                             cGGGCTTGTAATGGGGTAAGTGATAACAGATGTCTGGAAATATATGGGCAAATCCAGTTATCATCATGGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTGAATCGAATCCGaa                                                                                                                                                                                     <  2:419938/122‑1 (MQ=255)                                                                                                                                                                                               ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAAtt                                                                                                                                      <  1:145483/167‑1 (MQ=255)                                                                                                                                                                                               agCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACTTTAACCTTTCCCAATTTGAAACTGATGGCCAAtt                                                                                                                                      >  2:145483/2‑167 (MQ=255)                                                                                                                                                                                                     aaaTGGATTAAGTGATAACAGAAGTCGGGAAACATAGGCGCAAATCCAGTTATCCTCATTGGTCCAGCGTCAGCTGATGCCGTTGCATTCACTAAAGACTTATTCGAAGCCGAAGATCGTGAAATCGTTAACCTTTCCCACTTTGAAACTGATGGACAATTACCAGCTGGCCAAGATTCACTACGAACTCAGATTCTTAATCAGAGGACGCTAATCCATCTTGACCTCCAATGGGTATTAATTGATAAcc                                             >  2:200695/2‑250 (MQ=255)                                                                                                                                                                                                       aTGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCACAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGCAGCCAATCAACCTTGACCTCCAATGGGTATTAATTGATAAACaa                                           >  1:440629/1‑250 (MQ=255)                                                                                                                                                                                                           gTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGCATTAATTGATAACCAAACTGc                                      >  1:373618/1‑251 (MQ=255)                                                                                                                                                                                                                            gTCTGGAAATATAGGGGTAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCTAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCTGCAAATATTCACTAAGCTCTCTGATTAATAATAAGAGGACGCCAATCCACCTTTACCTCCAATGGGTATTAATTGATAGCCAAACTGCAGTTTGCGTGTCACCa                      <  1:86234/250‑1 (MQ=255)                                                                                                                                                                                                                                  aaaTATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAAt               >  1:242624/1‑251 (MQ=255)                                                                                                                                                                                                                                      ataGGGGCAAATCCAGTTATTATCATTGGTTGTGCGGCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTTATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAAc           <  2:242624/251‑1 (MQ=255)                                                                                                                                                                                                                                      ataGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTaa            <  1:437829/250‑1 (MQ=255)                                                                                                                                                                                                                                          gggCAAAATCAGTTATCAACATTGGTTGGGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGGTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACatta       <  2:373618/251‑1 (MQ=255)                                                                                                                                                                                                                                           ggCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGCAACTGATGGCCAATTACTCGTCGGCAAATATTCACTAAGCACTCTGCTTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGTGTATTAATTGATAACCAAACTGCAGATTGCGTGACACCAGCGCAATTAAAattat      >  2:240792/1‑251 (MQ=255)                                                                                                                                                                                                                                            gCAAATCTAGTTATCATTATTGGTTGGGCGTCAGCTTATGCCGTGGAATTCAACAAAGATTTAATCGAAGCCGAAGAGCGTGAAATCGTTAACCTGGCCCAATTGTAAGCTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGTCAATCCACCTTGACCTCCAATGGGTATTAATGGATAACCAAACTGCAGTTTGCTTGACACCAGTGCAATTAATattatt     <  2:197601/251‑1 (MQ=255)                                                                                                                                                                                                                                               aaTCCCGTTATCATCATTGGTTCTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGACGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGCTGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGTTTAATAATAAGAGGACGCCAATCCACCTTGCCCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTACCATTATTAgg  >  2:213572/1‑251 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                                      GGAGTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATCCAGTTATCATCATTGGTTGTGCGTCAGCTTATGCCGTTGAATTCAACAAAGATTTAATCGAAGCCGAAGATCGTGAAAACGTTAACCTTTCCCAATTTGAAACTGATGGCCAATTACCCGTCGGCAAATATTCACTAAGCACTCTGATTAATAATAAGAGGACGCCAATCCACCTTGACCTCCAATGGGTATTAATTGATAACCAAACTGCAGTTTGCGTGACACCAGAGCAATTAACATTATTAGG  >  NC_000913/3187183‑3187671

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.