Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,252,952	IS2 (–) +5 bp	coding (42‑46/357 nt)	yhaI →	putative inner membrane protein

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3186096 =	NA (NA)	34 (0.930)	32/488	0.2	97.1%	noncoding (1/1331 nt)	IS2	repeat region
	?	NC_000913	3252952 =	1 (0.030)					coding (42/357 nt)	yhaI	putative inner membrane protein
*	?	NC_000913	= 3187426	NA (NA)	23 (0.630)	22/488	0.6	95.8%	noncoding (1331/1331 nt)	IS2	repeat region
	?	NC_000913	= 3252956	1 (0.030)					coding (46/357 nt)	yhaI	putative inner membrane protein

CAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGC  >  NC_000913/3185851‑3186340                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       caaTACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACt                                                                                                                                                                                                                                                   >  1:425097/1‑251 (MQ=255)    tACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAGTTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGc                                                                                                                                                                                                                                                >  1:361353/1‑251 (MQ=255)          cAGCTATCAGGCACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTg                                                                                                                                                                                                                                          >  2:278115/1‑251 (MQ=255)           ggCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCAAAACAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGACCACATCATTAACGGATTACTGATAAGTGGCTCAGGTGTATCAAAAATTAAAATTAACCCCACTAAGCGAATTGATTAAAACTCTTTATTGTTCATTATCCGTTATTAGACTGGCCCCCtt                                                                                                                                                                                                                                          >  2:89950/2‑249 (MQ=255)             atATCATGAACTTAAATTCAAAGCGCGTATGGAATCCCCACGCAAAGATGCCACCCCGGTCAACGTTTACGCTCAGGTTGACTACAAGATTGCTTAAGAGTAAATGCATTTGGCCGGGGTCGCCCGGCCACATCATAAACGTATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTACCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAAt                                                                                                                                                                                                                                       <  2:80817/250‑1 (MQ=255)                    gAACTGAATTTCAAATCGCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGAGCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑Ga                                                                                                                                                                                                                               <  1:253417/251‑1 (MQ=255)                                aaaGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATc                                                                                                                                                                                                                    >  1:37089/1‑250 (MQ=255)                                      cGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATAGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAATTTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTAATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACAAATATCACTTaa                                                                                                                                                                                                              >  2:368355/1‑250 (MQ=255)                                        tATGGAATCCCCAAGCAAAGATGCAACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAACTATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAAt                                                                                                                                                                                                            >  2:231954/1‑250 (MQ=255)                                                       cAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAAt                                                                                                                                                                                            >  1:146761/1‑251 (MQ=255)                                                            aTGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAg                                                                                                                                                                                       <  1:192298/251‑1 (MQ=255)                                                                      gggCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAAGATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACt                                                                                                                                                                             <  1:43117/251‑1 (MQ=255)                                                                          aaCGATTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATAATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATCAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTAGTGTTCATTATACGTTATTAGACTGGCCCCCTGAATCTCCATAACAAC‑AATATCACTTAAATAAGTGATAATCTAAATAATAGTTTTTAGACTA‑GTc                                                                                                                                                                        >  2:121235/1‑251 (MQ=255)                                                                                       aGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAAc                                                                                                                                                              >  2:438458/1‑248 (MQ=255)                                                                                           tgaatACAAGCTTGATTACGAGTAATGGTAATTGGGCGGGATCGAGAGGCAACATCATTAAAGGATTAATGATAAGTGGATGAGATGTAGAAACAATTCAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATAATCAGTTATTAGACAGGCCCCGTGACTGTCCA‑GACAAACAATGTCACTTAAATACGAGATAGTCATAATAATAGTATTTAGACTAAGCCAT‑GGTGAACAgatgat                                                                                                                                                       >  1:368953/5‑251 (MQ=255)                                                                                                   aGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCt                                                                                                                                               <  2:37089/251‑1 (MQ=255)                                                                                                                        aTTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGAc                                                                                                                          >  1:206129/1‑251 (MQ=255)                                                                                                                            ggCCGTGTTCGCCAGGACACATCATTAACGGCTTAGGTATAAGTGGATCAGATGTATAAAATATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTAATTAGACTGGCCCCCTAAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGACTTAATACCAGTATTTTGACTA‑GTCATTGGAGAACAGATTATTGATATCTTAGGGACGGAGAAACTCAGaaggac                                                                                                                      >  2:220485/1‑246 (MQ=21)                                                                                                                                gggTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTAcc                                                                                                                  >  1:177667/1‑251 (MQ=255)                                                                                                                                               catcatTAACGGATTAGTGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑TACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGCTGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACTCAGGaac                                                                                                        >  2:68275/1‑245 (MQ=255)                                                                                                                                                                  ttAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATCAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGCCGTCTGACCACACAGGAAAAGATCGCAATTGTTCAGCagatct                                                                                >  2:38088/2‑248 (MQ=255)                                                                                                                                                                      gTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAAGTGTTCAGCAGAGCTTTGa                                                                            <  2:206129/251‑1 (MQ=255)                                                                                                                                                                             aGATGTGTAAAAAATTAAAATTAACCCCAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCAGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCCCTTAAATAAGTGATAGTCTTAATCCTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGACCTTTGAACCgggg                                                                     >  2:176998/1‑251 (MQ=255)                                                                                                                                                                                                    aCCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTAACCGTTATAAGACAGTCCCCCTGAATCTCCA‑GACAACCAATATCACTTAATTAAGTGATCGTCTTAATACCAGTTTTTCGACTA‑GTCATTGTAGAACAGATGATTAATGACTTAGGGCCGGAGACCAGCAGCCGGCGTACCACACAGAACATAATCGCAATTGATCCGCAGAGCTTTGAACCGGAGATAACTGACTCCCTCGTTGCCCg                                              >  2:282851/1‑251 (MQ=12)                                                                                                                                                                                                      cacaATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGc                                                                                                 <  2:120087/198‑1 (MQ=255)                                                                                                                                                                                                      cacaATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGc                                                                                                 >  1:120087/1‑198 (MQ=255)                                                                                                                                                                                                            aaGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTAGTTGCCCGGCAACATg                                      <  1:438458/251‑1 (MQ=255)                                                                                                                                                                                                                   ttGATTAAAAATATTTATAGTTCATTATCGGTTAGTAGACTGACCCCCTGAATTTCAA‑GACAAACAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GAAATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGACAATATCGCAATTGATAAGCAGAGCTTTGAACCGGAGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGCagc                               >  2:77486/1‑251 (MQ=14)                                                                                                                                                                                                                         aaaaaTATTGATTGTTCATTATCAGTTATTAGACTGGCCCATTGAATCACCA‑GAAAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTGAGTCTA‑GTCAGTGGAGAACAGATGAGTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTCCCACACAGGAAAAGATCGCAATTGTTCAGCAGAGTTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTCGcagccag                         <  2:361353/251‑1 (MQ=14)                                                                                                                                                                                                                                           ttATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAACGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCg       >  2:58663/1‑251 (MQ=18)                                                                                                                                                                                                                                                cGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGCGAACAGATGATTGATGTCTTAGGGCCGGCGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGc  >  1:251108/1‑251 (MQ=14)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                       CAATACCAACAGCTATCAGGAACTGAATTTCAAAGCTCGTATGGAATCCCCAAGCAAAGATGCCACCCCGGGCAACGTTTACGCTCAGGCTGACTACAAGATTGCTTACGAGTAATCGCATTTGGCCGGGTTCGCCCGGCCACATCATTAACGGATTAATGATAAGTGGATCAGATGTATAAAAAATTAAAATTAACCACAATAAGCGAATTGATTAAAAATATTTATTGTTCATTATCCGTTATTAGACTGGCCCCCTGAATCTCCA‑GACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTA‑GTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGC  >  NC_000913/3185851‑3186340

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.