Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,235,323	IS2 (+) +5 bp	coding (386‑390/720 nt)	fadR →	DNA‑binding transcriptional dual regulator FadR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1235323 =	0 (0.000)	20 (0.760)	19/490	0.4	100%	coding (386/720 nt)	fadR	DNA‑binding transcriptional dual regulator FadR
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region
*	?	NC_000913	= 1235327	0 (0.000)	15 (0.570)	13/490	0.7	100%	coding (390/720 nt)	fadR	DNA‑binding transcriptional dual regulator FadR
	?	NC_000913	= 1469240	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

CTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAA  >  NC_000913/1468997‑1469463                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                cgtaggCCAGAGAAATATCTGGCTGGCTGGTACACCATGTTGCCGGGCAACTAGGGAGACCTTCAGCTCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTGCTTGTGTTGTACGCCTATTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGCCGAGTTGATAAACGAGTAGTAAGATTTTCACTGAGTTTAGTCATATTGGTGGGCAGGAGATTGAGGAGGCCAGTCTAAGCCCCa                                                                                                                                                                                                                          <  2:239914/246‑1 (MQ=2)                  aCTGGCTGGCTGCTGCACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGa                                                                                                                                                                                                         <  1:133099/251‑1 (MQ=39)                      gctggcggCTACACCATGGTGCCGGTCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACAGCAATCATCTGTTCTCCAATGACTAGTCGAAAAACGAGTATTAAGACTATCACTTATTTAAGTGATATGGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGTACTTACTTATGCTGATTcc                                                                                                                                                                                                     <  2:100405/251‑1 (MQ=14)                        tggctgCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCgg                                                                                                                                                                                                   >  2:134714/1‑251 (MQ=255)                                          gCCGGGCGACGAGGGAGACCGTCAACCCCGGTTTAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTGCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGTCTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACACCAAAACTTACTTATGCTGAGTCCGGTTTGAAAATTGTtaata                                                                                                                                                                                  <  2:71875/250‑1 (MQ=14)                                                                             aaGCTCTGCTGAACTATTGCGATCTTTTCCTGTGTGGGACGCTGTCTGCTTTTCTCCGGCCCTAAGACAACAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTAGCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCATTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAAGTGTTAATAAAGGTACGTGTAGATCCAATTGGTCAACGCAACAGt                                                                                                                                              <  2:188536/251‑1 (MQ=18)                                                                              aGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGGACGCCGTCTTCGTTTCTCCGGCCCTAAGACAACAATCATCTGTTCTCCAATTACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGGGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTTCTTATGCTGATTCCGGTGCGAAAATTGTTAATATAGGTACTTGTAGATTCAATTGGTCAACGCAACAGtt                                                                                                                                             <  2:210389/251‑1 (MQ=18)                                                                                    gctgAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTAtg                                                                                                                                          <  2:29563/248‑1 (MQ=255)                                                                                                            gtgtgGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGttt                                                                                                                 <  1:134714/249‑1 (MQ=255)                                                                                                                              gTTTCTCAGGCCATAAGACAACACTCATCTGTTCTCCAATCCCTAGTCTAAAAACTAATATTACGACTATCACTTCTTTAAGTGATATTGGTTGTCTGGAGATTCAGCGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAATATTGTTAATAAAGGTACTTGTCGATTCAATTGGTCAACGCAACAGTTATGTGACAACATGGGGTTGCGGAGGTTTTTTGTGTGAGACGAACAttt                                                                                             >  2:15938/1‑251 (MQ=25)                                                                                                                                                                              tAAAAACTAGTATTAAGACTATCCCTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTAAGGAAGAACGGAAc                                              >  2:195937/1‑250 (MQ=255)                                                                                                                                                                                                               gTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAg                                                                             <  2:78051/186‑1 (MQ=255)                                                                                                                                                                                                               gTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCACAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGCTTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAg                                                                             >  1:78051/1‑186 (MQ=255)                                                                                                                                                                                                                    atTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAAc        <  1:248921/250‑1 (MQ=255)                                                                                                                                                                                                                          tGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGaa  <  1:145709/250‑1 (MQ=255)                                                                                                                                                                                                                                                    |                                                                                                                                                                                                                                CTTACGCCAGAGAAATAACTGGCTGGCTGCTACACCATGTTGCCGGGCAACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAA  >  NC_000913/1468997‑1469463

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.