Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,235,323	IS2 (+) +5 bp	coding (386‑390/720 nt)	fadR →	DNA‑binding transcriptional dual regulator FadR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1235323 =	0 (0.000)	20 (0.760)	19/490	0.4	100%	coding (386/720 nt)	fadR	DNA‑binding transcriptional dual regulator FadR
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region
*	?	NC_000913	= 1235327	0 (0.000)	15 (0.570)	13/490	0.7	100%	coding (390/720 nt)	fadR	DNA‑binding transcriptional dual regulator FadR
	?	NC_000913	= 1469240	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region

AGAAAAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCAC  >  NC_000913/1467661‑1468157                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        ataaaaaCGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTg                                                                                                                                                                                                                                                        <  1:18358/249‑1 (MQ=255)        cgcgCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGAAAAGAACCCTGGATTTGc                                                                                                                                                                                                                                                 >  1:773/1‑251 (MQ=255)          cgcTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGTCAAGAACCCTGGATTTGccc                                                                                                                                                                                                                                               <  1:188760/251‑1 (MQ=255)                       gTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATGTGAATGGAGATACGATAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTGTGCTGGAAATTGGCAGTGGCGCTACGCTAAGAATTCAGGATATTATCAGTTTGGAACGTCATGGGACAAGAACCTTGTATGTTCCCCTATATTTCCAGa                                                                                                                                                                                                                                  <  2:255674/251‑1 (MQ=255)                                                           taCCAGCGTTATTGTCGTTAGTTGTGAGTCGTCGGTATATCTGAATGGAGATACGACAAACAGCGGAGAATTCCCTCTGGGGTTTGCCGGTGTTATTCGGGTACAGTATAAAGCTTTGCTGGAAATTTGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATTGGACAAGATCCCTGGATTTGCCCCTATATTTCCAGCCATCTGTTAGCACTTAACCCATTagaactcctctg                                                                                                                                                                                               <  2:107848/250‑12 (MQ=255)                                                                       tGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTc                                                                                                                                                                                   <  1:83399/250‑1 (MQ=255)                                                                                                    tgaatgcagcctcGACAACCAACGGAGCATTCCCTCTAGAGATTGCCGGGGTTATTCGGCTAAAGGATAAAGCTTTGCTCGCCTTTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGACCATCCTGGGACAAGAACCCTGGATTTGCCCCTCTATTTCCAGTCATCTGTTATCACTTACCCCATTACCAGCCCGCTGCCGCAGATATTCCCGTGGCGCGCGATAACCCAGCGCACTATg                                                                                                                                                     >  2:111708/13‑251 (MQ=21)                                                                                                                                               tGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGtt                                                                                                           <  1:158403/250‑1 (MQ=255)                                                                                                                                                                                gttGGAAATTTGCAGTGGCGCTGTGCTAACAATGCAGGATTTTGACAGTTTTGAACTTCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGTCATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCTGTGGCGAGCGAGAACCCAGCGCACTATGCGTATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGATCTTTGCTTCCGTTTACCCTTCTGGTTTGGGCAt                                                                          <  2:151216/248‑1 (MQ=17)                                                                                                                                                                                    gAAATTGGCAGTGGCGCTCCGCTAACAATGCAGGATATTGACAGTTTTGACCATCATGGGACAAGAACACTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATAc                                                                     >  1:102726/1‑251 (MQ=255)                                                                                                                                                                                         tGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGCACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCCGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTTCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATg                                                                >  1:101952/1‑251 (MQ=255)                                                                                                                                                                                                                                     aaCATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGtt                    <  2:145418/251‑1 (MQ=38)                                                                                                                                                                                                                                     aaCATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGAGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGtt                    >  2:202633/1‑251 (MQ=38)                                                                                                                                                                                                                                                     aaCCCTTGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCTCTGCCGCTGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATTTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCggtttccgc    <  2:53537/251‑8 (MQ=255)                                                                                                                                                                                                                                                       cccTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCAc  >  1:122911/1‑251 (MQ=11)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                        AGAAAAACGCGCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTCCGCAC  >  NC_000913/1467661‑1468157

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.