japonet
What is S japonicus

Plasmid Resources
    PlasmidID Plasmid name Description Note Reference Distribution status
MAP SEQ 1 pKF14 ura4sj gene cloned into pGEMT-easy vector (Promega). pGEMT-ura4sj (1) Furuya and Niki 2009
Available
MAP SEQ 2 pKF15 ura4sj truncated gene cloned into pGEMT-easy vector (Promega), pKF14 derivertive. pGEMT-ura4sj-d (1) Furuya and Niki 2009
Available
MAP SEQ 3 pKF35 ade6sj gene cloned into pGEMT-easy vector (Promega). pGEMT-ade6sj (1) Furuya and Niki 2009
Available
MAP SEQ 4 pKF39 ade6sj gene deleted by ura4sp (pKF35 derivertive). pGEMT-ade6sj::ura4sp (1) Furuya and Niki 2009
Available
MAP SEQ 5 pKF40 ade6sj mutated gene (Ser427STOP(TGA)) cloned into pGEMT-easy vector (Promega). pGEMT-ade6sj-domE (1) Furuya and Niki 2009
Available
MAP SEQ 6 pSJU1 An ura4sj gene was cloned into HindIII site of pUC19 vector in which PmeI-AscI-NotI-PacI adaptor was inserted. (2)Aoki et al., 2010
Available
MAP SEQ

7

 pSJK1 A kanMX6 cassette was cloned into EcoRI site of pUC19 vector in which PmeI-AscI-NotI-PacI adaptor was inserted. (2)Aoki et al., 2010
Available
MAP SEQ 8 pSJU11 RS1 fragment was cloned into PacI site of pSJU1. RS1-containing plasmid (2)Aoki et al., 2010
Available
MAP SEQ 9 pSJK11 RS1 fragment was cloned into PacI site of pSJK1. RS1-containing plasmid (2)Aoki et al., 2010
Available
MAP SEQ 10 pSJK101 An ade6sj gene was cloned into NotI-PmeI site of pSJK11. RS1-containing plasmid (2)Aoki et al., 2010
Available

(1) Furuya, K., and Niki, H. (2009). Isolation of heterothallic haploid and auxotrophic mutants of Schizosaccharomyces japonicus. Yeast 26, 221-233.
(2) Aoki, K., Nakajima, R., Furuya, K., and Niki, H. (2010). Novel episomal vectors and a highly efficient transformation procedure for the fission yeast Schizosaccharomyces japonicus. Yeast 27, 1049-1060.
     
 

Microbial Physiology Laboratory (Niki Laboratory)
Department of Gene Function and Phenomics, National Institute of Genetics

1111-1 Yata Mishima Shizuoka Japan P.O. 411-8540