Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,995,620	IS5 (–) +4 bp	intergenic (‑261/‑32)	uvrY ← / → yecU	DNA‑binding transcriptional activator UvrY/protein YecU

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1397238	NA (NA)	11 (0.650)	9/294	0.5	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	= 1995623	0 (0.000)					intergenic (‑264/‑32)	uvrY/yecU	DNA‑binding transcriptional activator UvrY/protein YecU
*	?	NC_000913	1995620 =	0 (0.000)	9 (0.530)	9/294	0.5	100%	intergenic (‑261/‑35)	uvrY/yecU	DNA‑binding transcriptional activator UvrY/protein YecU
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

CATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCAGTTTTTAAAAACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTT  >  NC_000913/2289866‑2290319                                                                                                                                                                                                                                                        |                                                                                                                                                                                                               cATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGAGACAAGAAAATCTCTTTTCTGGTCTGTCTGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATa                                                                                                                                                                                                             <  1:416954/251‑1 (MQ=14)                  aaGGGCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTTCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGGCTGACGGCTCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGGCTCATGATGAACCCTGTTCTATGGCTCCGGATGACAAACAGGATCTCATAGCGGTGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAg                                                                                                                                                                                             <  1:241004/250‑1 (MQ=11)                               aTTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCgg                                                                                                                                                                               <  1:71521/251‑1 (MQ=255)                                                      gCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAag                                                                                                                                                                     >  1:24602/1‑238 (MQ=255)                                                         ngATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAAcc                                                                                                                                                         >  1:86477/2‑247 (MQ=255)                                                                           tttGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTg                                                                                                                                                                                                       <  1:18862/183‑1 (MQ=35)                                                                                cATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCt                                                                                                                               >  1:27059/1‑250 (MQ=255)                                                                                    gCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGACGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTACAGATGACACACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGa                                                                                                                           >  1:393977/1‑250 (MQ=255)                                                                                                              aaaaTCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCATGGACTTGGTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGAGATTGCGGAACGACAAAGAGAAAAAGAACTAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAg                                                                                                        <  1:379355/243‑1 (MQ=255)                                                                                                                                        cTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGaaa                                                                                                                         >  1:8744/1‑200 (MQ=255)                                                                                                                                                                                    tgatgaACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGAATTGTTCGGCCCTCCCCTAAAGCTGTAGccac                                                                                                                                                                                                >  1:189154/1‑85 (MQ=11)                                                                                                                                                                                             cTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCTCCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCttt                                                                                                                                     <  1:380568/135‑1 (MQ=255)                                                                                                                                                                                                 tNNATGGCTNNAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAag                                                                                                                                                                     <  2:24602/99‑1 (MQ=255)                                                                                                                                                                                                          ccAGATGACACANATGATCTCATATCAGGGACTTGTCCGTACCCNCNATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAANAGAACCATATNGATNNATTGNGNTNTCATCCTATGAAATTAATTGCTGTTAAAANCATTGGGTACAGAAAATACCCATNNCTNNNTCNNCGGAg                                                                 <  2:324810/190‑1 (MQ=255)                                                                                                                                                                                                          ccAGATGACAAACCTGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAaga                                                                                                                                                                    >  1:133446/1‑91 (MQ=255)                                                                                                                                                                                                          ccAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAAAAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAg                                                                 >  1:324810/1‑190 (MQ=255)                                                                                                                                                                                                            ccggtgacctgatttcttttggtCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCAGTTTTTAAAAACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGttttt  <  1:355781/229‑1 (MQ=255)                                                                                                                                                                                                                            ctcATATCAGGGACTTGTTCGCTCCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGNACCNGATTNATNCATTGAGCTTTCATCNTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCNNTNNNTNCATACCCg                                                                    <  2:304464/169‑1 (MQ=255)                                                                                                                                                                                                                            ctcATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGCGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTCAAAGCATTGGGTACCGAAAATACCCATAGCTCCATACCCg                                                                    >  1:304464/1‑169 (MQ=255)                                                                                                                                                                                                                              cATATCAGGGACTTGTTCGCACCTTNCATAACGCTNNAGNCACCANANNNGNNNTTNCGGAACGACAAAGAGAAACNGANNCAGATTNATGCATTGAGCTTTCATCCTATGaaa                                                                                                                         <  2:101496/114‑1 (MQ=255)                                                                                                                                                                                                                              cATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGaaa                                                                                                                         >  1:101496/1‑114 (MQ=255)                                                                                                                                                                                                                                                gCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTCAGGAACGACAAAGAGCAACAGAAAAAGATTGCTGCATTGAGCTTTCATCCTATGAAATTCATTGCTGt                                                                                                              >  1:166320/1‑107 (MQ=255)                                                                                                                                                                                                                                                     ttCCATAACGCTGTAGCCACCAGAACAGATATTGCGGCACGCCAAAGAGCCACCGAAACAGATTGATGCATTGAGCTTTCATCCTAt                                                                                                                             >  1:165406/1‑87 (MQ=255)                                                                                                                                                                                                                                                       ccATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGAAAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCAGTTTTTAAAAAc                                                  >  1:279255/1‑160 (MQ=255)                                                                                                                                                                                                                                                        |                                                                                                                                                                                                               CATGGTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCATAACGCTGTAGCCACCAGAACAGATATTGCGGAACGACAAAGAGAAACAGAACCAGATTGATGCATTGAGCTTTCATCCTATGAAATTAATTGCTGTTAAAAGCATTGGGTACAGAAAATACCCATAGCTCCATACCCGGAGTCAGTTTTTAAAAACTGTTTAAAGAAATGCACAAGTATTGTGATTGATTTTTTAGTTGTTTTT  >  NC_000913/2289866‑2290319

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.