Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,995,620	IS5 (–) +4 bp	intergenic (‑261/‑32)	uvrY ← / → yecU	DNA‑binding transcriptional activator UvrY/protein YecU

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 1397238	NA (NA)	11 (0.650)	9/294	0.5	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	= 1995623	0 (0.000)					intergenic (‑264/‑32)	uvrY/yecU	DNA‑binding transcriptional activator UvrY/protein YecU
*	?	NC_000913	1995620 =	0 (0.000)	9 (0.530)	9/294	0.5	100%	intergenic (‑261/‑35)	uvrY/yecU	DNA‑binding transcriptional activator UvrY/protein YecU
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

CTGAAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATGGCGAAACTGAAGTCAGCGAAAGGGAAGAAATTTCTCTTTGG  >  NC_000913/1397000‑1397414                                                                                                                                                                                                                                               |                                                                                                                                                                                 cTGAAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAATTGATACGTCAGTGGGCGCGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGCATTTCCCCGCTTATTCGCACCTTCCCTCACTAATCa                                                                                                                                                                       >  1:364339/1‑250 (MQ=255)                       cGATGTTATTCACTCTTGCCTACCTGTTTCGGGCGGACCAAATTATGCGTCTGTGGTAGAGATCTCACTAAAAACTTGGGATAACGCCGTAAATTGCGAAGAAACGGTCTAAATAGGCGGATTCGAAGCATTCACGGGAGAAAAAATCGGCGCAAATATGAAGAAATTTAATGACTTAGTCAGCCGAGAAGAATAACCCCGCGTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTTTAAAGCCAGCt                                                                                                                                                <  1:65905/251‑1 (MQ=255)                                 cACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAgaggtgag                                                                                                                                      <  1:339913/251‑1 (MQ=255)                                 cACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAgaggtgag                                                                                                                                      <  1:345939/251‑1 (MQ=255)                                           aaCCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCAACTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCag                                                                                                                              >  1:183586/1‑249 (MQ=255)                                                        cGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCGTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGc                                                                                                                           <  1:62143/239‑1 (MQ=255)                                                                  gATACGTCAGTGGGAGAGATCTCACGAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTGCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTGTCAGAGCAGACAACGGTGAAATGTCATgg                                                                                                     <  1:350203/251‑1 (MQ=255)                                                                     aCGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTGAAATGGCGAAGAAACGGTCTAAAGAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGGAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCGCCGTCCCTAACGAAGCAGCGCGTGGATTGTAAATCCAGCTAAGGGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTa                                                                                                   <  1:174011/250‑1 (MQ=17)                                                                                agagaTCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTTGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACCGTGAAATGTCATGGTATTGTTACGTTTa                                                                                       <  1:147730/251‑1 (MQ=255)                                                                                        cACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCCTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACa                                                                                >  1:431243/1‑250 (MQ=255)                                                                                                                   aTGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTAcatc                                                    <  1:154490/251‑1 (MQ=255)                                                                                                                      gCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAACTGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCctaacta                                                                                                                                                                           >  1:338785/1‑129 (MQ=14)                                                                                                                      gCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGATCAAACATGAAGCAATGAAATGACTGAGTCAGCCGAGAAGCATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTg                                                                                                                >  1:75048/1‑188 (MQ=255)                                                                                                                              aCGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATGGc                                         <  1:91162/251‑1 (MQ=255)                                                                                                                                                       naTTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGtt                                                                                              >  1:269258/2‑173 (MQ=255)                                                                                                                                                                       aaTCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATGGCGAAACTGAAGTCAGCGAAAGGGAAGAAATTTCTCTTTgg  <  1:385199/249‑1 (MQ=255)                                                                                                                                                                         ncGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTAATTCGCACCTTCCCTAACTAATCAATGCGTTGATCGTAAATCCAGCTAAGCGGTGAGGTTTTCAGAGAAGAc                                                                                                                       >  1:50504/2‑130 (MQ=21)                                                                                                                                                                           ggCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGCGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCACTGCGTTGCTTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACa                                                                                >  1:69204/1‑167 (MQ=255)                                                                                                                                                                                    aTNNNNNAATNAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGtt                                                                                              <  2:269258/144‑1 (MQ=255)                                                                                                                                                                                                        aGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCTTTAACTAATCAATGCGTTGATTGTa                                                                                                                                                         >  1:273626/1‑65 (MQ=18)                                                                                                                                                                                                                gagaAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAgn                                                                                                                                                 <  1:45812/65‑2 (MQ=255)                                                                                                                                                                                                                                    tnCGNACCTTNCCTANCTAATCAATGCGTTGNTTGTAAATCCAGCTAAGAGNNGNGGTTTNCagag                                                                                                                            <  2:183586/66‑1 (MQ=255)                                                                                                                                                                                                                                               |                                                                                                                                                                                 CTGAAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAACTAATCAATGCGTTGATTGTAAATCCAGCTAAGAGGTGAGGTTTTCAGAGCAGACAACGGTGAAATGTCATGGTATTGTTACGTTTAGGTAACAAGAAATTTGTCTGCACAAGGATTACATCATGATTATGGCGAAACTGAAGTCAGCGAAAGGGAAGAAATTTCTCTTTGG  >  NC_000913/1397000‑1397414

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.