Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	2,237,684	IS5 (+) +4 bp	coding (67‑70/1011 nt)	mglC ←	D‑galactose/methyl‑galactoside ABC transporter membrane subunit

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2237684 =	0 (0.000)	27 (1.540)	22/292	0.0	100%	coding (70/1011 nt)	mglC	D‑galactose/methyl‑galactoside ABC transporter membrane subunit
	?	NC_000913	2288919 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region
*	?	NC_000913	= 2237687	0 (0.000)	11 (0.630)	9/292	0.5	100%	coding (67/1011 nt)	mglC	D‑galactose/methyl‑galactoside ABC transporter membrane subunit
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region

TATTATTATCTCCTCTGAAATGCCTGAGTTGTTAGGGATAACAGACCGTATTCTGGTCATGAGCAATGGTCTCGTTTCCGGAATTGTCGATACAAAAACAACAACGCAAAACGAAATTCTGCGTCTTGCGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2237913‑2237684 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCC  >  NC_000913/2288919‑2289137                                                                                                                                                                                                                                                                                                                                                                                                                                                                    TATTATTATTTCCGCTGAAATGACTGAGTTGTGAGGGATAAAAGAACGTATTCTGGTCATGAGCAATGGTCTCGGTGCCGGAATTGTCGATACAAAAACAACAACGCAAAACGAAATTCTGCGTCGTGCGGCTTTGCACCGTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTGACTTACCTGAAAGAGGGCGGTATTTACGTCGTGCGTTTAGGGAAGGTGCGAATAAGCGGG                                                                                                                                                                                                          <  1:370328/250‑1                                   GGGATAACAGACCGTATTCTGGTCATGAGCAATGGTCTCGTTTCCGGAATTTTCGATACAAAAACAACAACGCAAAACGAAAGTCTGCGTCTTGCGTCTTTGCACCTGTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTGTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGG                                                                                                                                                                                                          <  1:311562/216‑1                                    GGATAACAGACCGTATTCTGGTCATGAGCAATGGTCTCGTTTCCGGAATTGTCGATCCAAAAACAACAACGCAAAACGAAATTCTGCGTCTTGCGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGATGGCGGTATTTACGTCGTTCTTTTAGGGAAGG                                                                                                                                                                                                                        >  1:409286/1‑201                                    GGATAACAGACCGTATTCTGGTCATGAGCAATGGTATCGTTTCCGGAATTGTCGATACACAAACAACAACACAAAACGAAATTCTGCGTCTTGCGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCT                                                                                                                                                                     >  1:186477/1‑251                                                            TGAGCAATGGTCTCGTTTCCGGAATTGTCGATACAAAAGCAACAACGCAAACCGAAATTCTGCGTCTTGAGTCTTTGCACCTTTACGATAAGGGGCTTCACATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGACGGCGCGAATAAGCGGCG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTC                                                                                                                                             >  1:191513/1‑251                                                                                  AATTGTCGATACAAAAACAACAACGCAAAACGAAATTCTGCGTCTTGCGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATTTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCC                                                                                                                       <  1:184070/251‑1                                                                                           TACAAAAACAACAAAGCAAAACGAAATTCTGCGTATTGCGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCAGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑ACATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAC                                                                                                              >  1:119883/1‑251                                                                                            ACAAAAACAACAACGCAAAACGAAATTCTGCGTCTTGCGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCAGTTCTTCATGTTTGAGCCGATTGTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAC                                                                                                              <  1:383130/250‑1                                                                                            ACAAAAACAACAACGCAAAACGAAATTCTGCGTCTTGCGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGA‑AAATTCTGCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTGTTTCTCCCGTAAATGCCTTGAATCATCCTATTTAGACC                                                                                                             <  1:74148/251‑1                                                                                             CAAAAACAACAACGCAAAACGAAATTCTGCGTCTTGCGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCn                                                                                                                                                        <  1:272037/207‑2                                                                                                   CAACAACGCAAAACGAAATTCTGCGTCTTGCGTCTTTGCACCGTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCTGTATTTACGTCGTTCTTTGAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTT                                                                                                      <  1:217747/251‑1                                                                                                                             CTTGCGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTAATTAACTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGCTTTTTTCTCCCGTAAATGCCTTG                                                                                                                               >  1:186853/1‑201                                                                                                                                 CGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGCAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTT                                                                         >  1:325507/1‑250                                                                                                                                        GCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGC                                                                                                                        >  1:125147/1‑196                                                                                                                                                      agtGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACG                                                   <  1:6228/248‑1                                                                                                                                                      AGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTGTCCTGAAAGAGGGCGGGATTTACGTCGTTCTGTTAGTGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCATCCTATTTAGACCGTTGCTTCTCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACG                                                   <  1:218578/251‑1                                                                                                                                                                CATGAGTGCGTGAAATAAGAAAAGGTGTCTTACTTACCTGAAAGAGGGCGGTGTTTACGTCGTTCTTGTAATGAAGGTGCTAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGC                                                                                                                                                         <  1:62977/139‑1                                                                                                                                                                     GTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTGTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGC                                    <  1:285666/251‑1                                                                                                                                                                         GTTAAATAAGAAAANTNTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGANGGTGCGAATANGCGNNG‑AAANTNTNCTCGGCTGACTCAGTCATTTCATTTCTTCNTGTTTGAGCCGATTTTTTCTCCCNNAANNNCCNNGAATC                                                                                                                           <  2:330651/160‑1                                                                                                                                                                         GTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGACGGTATTTAAGTTGTTATTTTAGGGAAGGTGAGAATAAGCGGGG‑AACTTCTTATCGGCTGACTAAGTAATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATC                                                                                                                           >  1:330651/1‑160                                                                                                                                                                                       GTTTTCTTNCNNNCNNGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAANAAGCGGGG‑AACTTCTNCTNGGCTNACTNAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTC                                                                                                        >  2:307345/1‑165                                                                                                                                                                                       GTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATGTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTC                                                                                                        <  1:307345/165‑1                                                                                                                                                                                       GTTTTCGTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGT‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTTATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGCTTCTTCGCCATTTAAGGCTGTATCCCCAGTTATTAGTGAGATCGCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTG                       <  1:258531/246‑1                                                                                                                                                                                              TACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTGAGGGAAAGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCTATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTACACCGCTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGATATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTG              <  1:407136/248‑1                                                                                                                                                                                                    CCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTT                                                                                                                                                >  1:156427/1‑112                                                                                                                                                                                                     CTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTn                                                                                                                                                            <  1:359296/99‑2                                                                                                                                                                                                       GAAAGAGGGCGGTATTTACGGCGTTCTTTTAGGGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGAATCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCC  >  1:96146/1‑251                                                                                                                                                                                                                                                                                                                                                                                                                                                                    TATTATTATCTCCTCTGAAATGCCTGAGTTGTTAGGGATAACAGACCGTATTCTGGTCATGAGCAATGGTCTCGTTTCCGGAATTGTCGATACAAAAACAACAACGCAAAACGAAATTCTGCGTCTTGCGTCTTTGCACCTTTAAGATTAGGGGCTTCCCATGAGTGCGTTAAATAAGAAAAGTTTTCTTACTTACCTGAAAGAGGGCGGTATTTACGTCGTTCTTTTAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2237913‑2237684 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GGAAGGTGCGAATAAGCGGGG‑AAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCC  >  NC_000913/2288919‑2289137

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.