breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 121 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_00091370,3710TC55.1% 60.2 / inf 236intergenic (‑323/‑16)araB/araCribulokinase/DNA‑binding transcriptional dual regulator AraC
*NC_00091370,7400TG53.1% 49.9 / 182.6 128G118G (GGT→GGGaraCDNA‑binding transcriptional dual regulator AraC
*NC_00091371,1750AG51.3% ‑4.6 / 237.1 149R263R (CGA→CGGaraCDNA‑binding transcriptional dual regulator AraC
*NC_00091370,2890GT51.2% ‑2.5 / inf 216intergenic (‑241/‑98)araB/araCribulokinase/DNA‑binding transcriptional dual regulator AraC
*NC_00091371,2140TC51.1% 7.2 / 224.5 139F276F (TTT→TTCaraCDNA‑binding transcriptional dual regulator AraC
*NC_000913581,3220CT49.6% 12.1 / 231.5 139A163A (GCC→GCTnohDputative DNA‑packaging protein NohD
*NC_00091370,4340CT49.1% 3.9 / inf 229N16N (AAC→AATaraCDNA‑binding transcriptional dual regulator AraC
*NC_000913581,3350CA48.3% 25.0 / 216.3 147L168M (CTG→ATG) ‡nohDputative DNA‑packaging protein NohD
*NC_000913581,3370GA48.2% 6.3 / 236.0 143L168L (CTG→CTA) ‡nohDputative DNA‑packaging protein NohD
*NC_00091371,0850TC47.5% 29.6 / 218.5 143S233S (AGT→AGCaraCDNA‑binding transcriptional dual regulator AraC
*NC_00091371,0790CT47.2% 12.1 / 224.3 143R231R (CGC→CGTaraCDNA‑binding transcriptional dual regulator AraC
*NC_00091371,0820TC46.9% 42.0 / 213.9 145I232I (ATT→ATCaraCDNA‑binding transcriptional dual regulator AraC
*NC_00091371,1090TC46.5% 43.2 / 215.8 144T241T (ACT→ACCaraCDNA‑binding transcriptional dual regulator AraC
*NC_00091370,5810CT46.2% 6.7 / 251.2 156V65V (GTC→GTTaraCDNA‑binding transcriptional dual regulator AraC
*NC_000913581,2910GA44.3% 49.9 / 140.9 124R153Q (CGG→CAG) ‡nohDputative DNA‑packaging protein NohD
*NC_000913581,2890AG44.2% 64.8 / 141.7 121K152K (AAA→AAGnohDputative DNA‑packaging protein NohD
*NC_000913581,2750GA43.5% 26.2 / 176.9 124V148I (GTT→ATT) nohDputative DNA‑packaging protein NohD
*NC_000913581,2900CA43.5% 50.0 / 162.0 124R153R (CGG→AGG) ‡nohDputative DNA‑packaging protein NohD
*NC_000913581,2950TC43.5% 53.7 / 167.7 124D154D (GAT→GACnohDputative DNA‑packaging protein NohD
*NC_000913581,3010CT43.5% 46.4 / 165.8 124I156I (ATC→ATTnohDputative DNA‑packaging protein NohD

Marginal new junction evidence (lowest skew 10 of 25 shown)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 = 364814695 (1.060)17 (0.240) 17/472 2.6 16.4% intergenic (+2/‑148) agrA/agrB small RNA AgrA/small regulatory RNA AgrB
?NC_000913 3648378 = 97 (1.350)intergenic (+3/‑150) agrB/arsR small regulatory RNA AgrB/DNA‑binding transcriptional repressor ArsR
* ? NC_000913 = 69674192 (1.030)12 (0.180) 8/432 4.5 16.6% intergenic (‑1/+15) metU/glnW tRNA‑Met/tRNA‑Gln
?NC_000913 697135 = 53 (0.810)intergenic (‑2/+378) metT/asnB tRNA‑Met/asparagine synthetase B
* ? NC_000913 1207800 =29 (0.320)13 (0.150)
+A
12/586 4.7 31.0% coding (300/630 nt) ycfK protein StfP
?NC_000913 1209610 = 29 (0.320)intergenic (‑491/‑75) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 409435078 (0.870)9 (0.120) 9/506 4.9 12.7% intergenic (‑78/+373) rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
?NC_000913 4094719 = 57 (0.740)intergenic (‑447/+4) rhaD/rhaA rhamnulose‑1‑phosphate aldolase/L‑rhamnose isomerase
* ? NC_000913 = 346904083 (0.930)10 (0.120) 9/548 5.3 11.1% coding (814/2694 nt) chiA endochitinase
?NC_000913 3469344 = 83 (1.000)coding (510/2694 nt) chiA endochitinase
* ? NC_000913 = 2357730118 (1.320)10 (0.130) 8/508 5.3 9.0% coding (827/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357926 = 100 (1.300)intergenic (+123/+116) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 = 128690650 (0.560)11 (0.130) 9/548 5.3 18.7% coding (198/276 nt) ychS putative uncharacterized protein YchS
?NC_000913 1287557 = 49 (0.590)noncoding (66/85 nt) tyrT tRNA‑Tyr
* ? NC_000913 = 2357664128 (1.430)8 (0.090) 8/566 5.8 6.6% coding (761/900 nt) rpnE inactive recombination‑promoting nuclease‑like protein RpnE
?NC_000913 2357848 = 102 (1.190)intergenic (+45/+194) rpnE/yfaU inactive recombination‑promoting nuclease‑like protein RpnE/2‑keto‑3‑deoxy‑L‑rhamnonate aldolase
* ? NC_000913 781577 =46 (0.510)9 (0.100)
+GAAG
8/580 5.9 12.6% noncoding (1/76 nt) lysQ tRNA‑Lys
?NC_000913 = 2521006 81 (0.910)noncoding (76/76 nt) valU tRNA‑Val
* ? NC_000913 = 4542690148 (1.660)7 (0.080) 7/570 6.2 5.2% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 110 (1.270)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit