breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Marginal read alignment evidence (highest frequency 20 of 182 shown, sorted by frequency from high to low)
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009132,304,5230GC75.0% 86.0 / 40.4 64intergenic (+130/+585)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_0009132,304,5220GT73.4% 71.4 / 36.9 64intergenic (+129/+586)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_0009133,583,9690GA71.4% 20.4 / 13.8 21W98* (TGG→TAG) insB6IS1 family protein InsB
*NC_0009134,040,6230GA64.7% 48.9 / 111.3 102noncoding (107/120 nt)rrfA5S ribosomal RNA
*NC_0009134,166,8560GA60.0% ‑1.1 / 38.5 35noncoding (198/1542 nt)rrsB16S ribosomal RNA
*NC_0009132,304,5370AG54.5% ‑3.6 / 78.4 67intergenic (+144/+571)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_0009132,304,5400CT52.2% 7.3 / 93.2 69intergenic (+147/+568)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_0009132,304,5490CT47.7% 30.4 / 85.3 65intergenic (+156/+559)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_0009132,304,5670CT39.7% 60.5 / 47.9 59intergenic (+174/+541)eco/mqoserine protease inhibitor ecotin/malate:quinone oxidoreductase
*NC_0009134,296,2680TC39.6% 35.8 / 48.0 49intergenic (+474/+168)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
*NC_0009131,224,0210AG37.1% 43.8 / 11.6 36intergenic (+572/+258)ymgJ/minEuncharacterized protein YmgJ/Z‑ring positioning protein MinE
*NC_000913786,8550AC35.0% 153.6 / 16.9 81G247G (GGT→GGGgpmA2,3‑bisphosphoglycerate‑dependent phosphoglycerate mutase
*NC_0009134,604,3440CT34.6% 81.9 / 20.8 52S62F (TCT→TTT) bglJDNA‑binding transcriptional regulator BglJ
*NC_0009131,679,3040AC34.2% 135.5 / 14.3 76Y293S (TAC→TCC) ydgHDUF1471 domain‑containing protein YdgH
*NC_0009131,292,4550CA33.3% 96.1 / 18.5 60intergenic (+90/+54)galU/hnsUTP‑‑glucose‑1‑phosphate uridylyltransferase/DNA‑binding transcriptional dual regulator H‑NS
*NC_0009132,367,3620AC32.3% 82.0 / 10.2 67T98P (ACC→CCC) arnCundecaprenyl‑phosphate 4‑deoxy‑4‑formamido‑L‑arabinose transferase
*NC_0009133,404,2150TG31.6% 196.2 / 10.2 98G244G (GGT→GGGyhdHacrylyl‑CoA reductase
*NC_000913354,2880TG31.6% 321.4 / 31.8 152V528G (GTG→GGG) prpEpropionyl‑CoA synthetase
*NC_0009132,664,8960TG31.3% 198.7 / 15.3 100G169G (GGT→GGGyfhRputative peptidase
*NC_0009132,483,2680TA31.1% 89.0 / 15.2 45L24F (TTA→TTTemrKtripartite efflux pump membrane fusion protein EmrK

Marginal new junction evidence (sorted from low to high skew)
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 4542682 =50 (0.400)19 (0.160) 19/460 3.2 31.6% intergenic (+49/‑433) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 4542996 = 34 (0.290)intergenic (+363/‑119) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 454269048 (0.390)17 (0.140) 17/460 3.4 30.6% intergenic (+57/‑425) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
?NC_000913 = 4542986 31 (0.260)intergenic (+353/‑129) fimE/fimA regulator for fimA/type 1 fimbriae major subunit
* ? NC_000913 = 275149NA (NA)10 (0.080) 10/478 4.6 10.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1108013 = 86 (0.690)coding (929/1158 nt) opgC osmoregulated periplasmic glucans (OPG) biosynthesis protein C
* ? NC_000913 = 15912NA (NA)8 (0.070)
+TTTTTCAA
8/462 4.9 NA noncoding (526/1345 nt) IS186 repeat region
?NC_000913 15922 = NA (NA)noncoding (536/1345 nt) IS186 repeat region
* ? NC_000913 781068 =129 (1.040)4 (0.050) 4/326 5.2 5.2% noncoding (1/76 nt) valZ tRNA‑Val
?NC_000913 = 2520930 58 (0.690)intergenic (‑258/‑1) gltX/valU glutamate‑‑tRNA ligase/tRNA‑Val
* ? NC_000913 = 1397238NA (NA)5 (0.040) 5/478 5.8 6.2% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1673611 75 (0.610)coding (99/102 nt) mdtU protein MdtU
* ? NC_000913 = 141192471 (0.570)3 (0.030) 3/426 6.2 5.1% coding (25/936 nt) ttcA tRNA cytosine(32) 2‑sulfurtransferase TtcA
?NC_000913 1434985 = 48 (0.440)intergenic (‑579/+200) ynaM/uspF protein YnaM/universal stress protein F
* ? NC_000913 3392288 =152 (1.230)3 (0.020) 3/464 6.4 2.0% intergenic (‑260/+170) tldD/yhdP metalloprotease subunit TldD/phospholipid transport factor YhdP
?NC_000913 = 4325879 148 (1.230)intergenic (‑138/+520) yjdN/yjdM PF06983 family protein YjdN/zinc ribbon domain‑containing protein YjdM
* ? NC_000913 2575553 =119 (0.960)3 (0.020) 3/464 6.4 2.5% coding (253/336 nt) eutS putative ethanolamine catabolic microcompartment shell protein EutS
?NC_000913 = 2709110 122 (1.020)coding (295/651 nt) rseA anti‑sigma‑E factor RseA