breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
MC JC 260,173 Δ13,782 bp IS5‑mediated proB[ykfC] 19 genes
RA 284,535 T→G A445A (GCT→GCG yagF → D‑xylonate dehydratase
RA 635,355 G→A R8* (CGA→TGA) 
N405N (AAC→AAT
ybdM ←
ybdN ←
ParB‑like nuclease domain‑containing protein YbdM
putative PAPS reductase/DUF3440 domain‑containing protein YbdN
RA 696,582 C→T noncoding (35/75 nt) glnV ← tRNA‑Gln
RA 811,531 G→A G384D (GGT→GAT) 
V4I (GTT→ATT) 
bioF →
bioC →
8‑amino‑7‑oxononanoate synthase
malonyl‑acyl carrier protein methyltransferase
RA 1,097,494 T→A intergenic (+665/+71) ycdU → / ← serX uncharacterized protein YcdU/tRNA‑Ser
RA 1,169,836 A→G L180P (CTG→CCG)  ldtC ← L,D‑transpeptidase LdtC
RA 1,189,980 A→G T156T (ACT→ACC phoP ← DNA‑binding transcriptional dual regulator PhoP
RA 1,301,992 A→T N271Y (AAT→TAT)  oppA → oligopeptide ABC transporter periplasmic binding protein
RA 1,306,736 T→G S325A (TCC→GCC)  oppF → murein tripeptide ABC transporter/oligopeptide ABC transporter ATP binding subunit OppF
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
RA 1,358,859 T→C Y110C (TAT→TGT)  puuP ← putrescine:H(+) symporter PuuP
RA 1,643,679 A→T L209Q (CTG→CAG)  ydfU ← protein YdfU
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,705,108 G→A G54R (GGG→AGG)  cnu → H‑NS‑ and StpA‑binding protein
MC JC 1,978,503 Δ776 bp insB5insA5 insB5, insA5
RA 2,040,433 C→A A319D (GCC→GAC)  msrP → protein‑L‑methionine sulfoxide reductase catalytic subunit MsrP
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,406,142 IS1 (+) +9 bp coding (492‑500/939 nt) lrhA ← DNA‑binding transcriptional dual regulator LrhA
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 3,388,041 T→G T50P (ACG→CCG)  aaeB ← aromatic carboxylic acid efflux pump subunit AaeB
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
RA 3,707,947 C→A intergenic (‑242/+669) dppA ← / ← proK dipeptide ABC transporter periplasmic binding protein/tRNA‑Pro
RA 4,132,697 G→A E28K (GAG→AAG)  metF → 5,10‑methylenetetrahydrofolate reductase
RA 4,223,638 T→C intergenic (‑10/‑190) iclR ← / → metH DNA‑binding transcriptional repressor IclR/cobalamin‑dependent methionine synthase
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,510,238 T→G intergenic (+445/+452) insO → / ← fecE IS911B regulator fragment/ferric citrate ABC transporter ATP binding subunit
RA 4,566,642 G→A S202L (TCG→TTG)  yjiN ← DUF445 domain‑containing protein YjiN

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 3423764–3424233 3424477–3424238 6–714 27 [26] [25] 27 [rrfD]–[rrlD] [rrfD],[rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 146734 =81 (0.890)28 (0.310) 26/598 2.3 25.7% intergenic (‑40/‑234) panD/rpnC aspartate 1‑decarboxylase proenzyme/recombination‑promoting nuclease RpnC
?NC_000913 = 1470516 NA (NA)noncoding (1221/1221 nt) IS30 repeat region
* ? NC_000913 = 14673582 (0.900)24 (0.260) 22/598 2.9 22.6% intergenic (‑41/‑233) panD/rpnC aspartate 1‑decarboxylase proenzyme/recombination‑promoting nuclease RpnC
?NC_000913 270541 = NA (NA)noncoding (1/1221 nt) IS30 repeat region
* ? NC_000913 273955 =NA (NA)69 (0.760) 45/598 0.8 66.3% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 679345 = 35 (0.390)coding (62/555 nt) ybeT Sel1 repeat‑containing protein YbeT
* ? NC_000913 = 275149NA (NA)65 (0.720) 46/598 0.7 62.5% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 679348 39 (0.430)coding (59/555 nt) ybeT Sel1 repeat‑containing protein YbeT
* ? NC_000913 = 275149NA (NA)34 (0.370) 28/598 2.1 27.4% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 691806 = 90 (0.990)intergenic (‑22/+68) ybeX/ybeY CorC‑HlyC family protein YbeX/endoribonuclease YbeY
* ? NC_000913 = 1096852128 (1.410)33 (0.360) 25/596 2.4 20.5% intergenic (+23/+713) ycdU/serX uncharacterized protein YcdU/tRNA‑Ser
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region
* ? NC_000913 1207790 =76 (0.840)50 (0.580) 38/566 1.1 38.2% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 90 (1.050)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780579 (0.870)58 (0.670) 41/566 0.9 41.3% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 90 (1.050)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 12994981 (0.010)94 (1.080) 63/574 0.2 99.0% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 = 1300693 NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 12994981 (0.010)33 (0.380) 26/574 2.2 97.1% intergenic (+253/‑69) ychE/insH21 MarC family putative inner membrane protein YchE/IS5 family transposase and trans‑activator
?NC_000913 1427599 = NA (NA)noncoding (1196/1196 nt) IS5 repeat region
* ? NC_000913 1299499 =NA (NA)107 (1.230) 67/574 0.1 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 1300694 = 0 (0.000)intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
* ? NC_000913 1300694 =0 (0.000)21 (0.240) 21/572 2.9 100% intergenic (+147/‑488) insH21/oppA IS5 family transposase and trans‑activator/oligopeptide ABC transporter periplasmic binding protein
?NC_000913 = 1428794 NA (NA)noncoding (1/1196 nt) IS5 repeat region
* ? NC_000913 = 1397238NA (NA)24 (0.260) 23/598 2.7 26.1% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 1996011 = 68 (0.750)coding (194/225 nt) yecF DUF2594 domain‑containing protein YecF
* ? NC_000913 = 1397238NA (NA)64 (0.700) 53/598 0.5 73.6% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 2176309 = 23 (0.250)coding (13/1263 nt) gatZ putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
* ? NC_000913 1399590 =68 (0.750)32 (0.350) 29/598 2.0 32.0% intergenic (‑64/+131) fnr/ogt DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
?NC_000913 2101749 = NA (NA)noncoding (1195/1195 nt) IS5 repeat region
* ? NC_000913 = 139959366 (0.730)30 (0.330) 26/596 2.3 31.2% intergenic (‑67/+128) fnr/ogt DNA‑binding transcriptional dual regulator FNR/methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 199601468 (0.750)33 (0.360) 30/596 1.9 32.7% coding (197/225 nt) yecF DUF2594 domain‑containing protein YecF
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 = 2070271NA (NA)73 (0.800) 50/598 0.6 68.2% noncoding (1/1331 nt) IS2 repeat region
?NC_000913 = 2389243 34 (0.370)coding (128/849 nt) yfbP uncharacterized protein YfbP
* ? NC_000913 = 217631223 (0.250)64 (0.700) 43/598 0.9 73.6% coding (10/1263 nt) gatZ putative tagatose‑1,6‑bisphosphate aldolase 2 chaperone
?NC_000913 = 2290113 NA (NA)noncoding (1/1195 nt) IS5 repeat region
* ? NC_000913 2288919 =NA (NA)29 (0.320) 25/598 2.5 30.9% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 2492975 = 65 (0.720)coding (280/1251 nt) frc formyl‑CoA transferase
* ? NC_000913 = 2290113NA (NA)30 (0.330) 26/596 2.3 31.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 2492978 65 (0.720)coding (277/1251 nt) frc formyl‑CoA transferase
* ? NC_000913 2389239 =33 (0.360)57 (0.630) 50/598 0.6 63.3% coding (124/849 nt) yfbP uncharacterized protein YfbP
?NC_000913 2996361 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region