Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,105,699	IS1 (+) +9 bp	coding (286‑294/312 nt)	ymdA →	uncharacterized protein YmdA

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	290634 =	NA (NA)	18 (1.160)	14/308	0.1	100%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	1105699 =	0 (0.000)					coding (286/312 nt)	ymdA	uncharacterized protein YmdA
*	?	NC_000913	= 291401	NA (NA)	12 (0.770)	8/308	0.4	100%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 1105707	0 (0.000)					coding (294/312 nt)	ymdA	uncharacterized protein YmdA

CGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/290808‑290634 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCG  >  NC_000913/1105699‑1105938                                                                                                                                                                                                                                                                                                                                                                                                                                 CGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCCCCTGGCAAGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAA                                                                                                                                                                       >  1:54271/1‑250     ATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAAn                                                                                                                                                                          <  1:218411/243‑2                          CGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACA                                                                                                                                                                                                            >  1:98736/1‑188                                             TGAATCTGNGNNNGNNCCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGNGGAGCAGCATGACAANGTNATCGNGCANTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGCTGTAATGCACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGn                                                                                                                                                                                  >  2:246522/1‑194                                             TGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAAGCGGTGGAGCAGCATGACAAAGGCATCGTGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGGCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGC                                                                                                                                                                                  <  1:246522/195‑1                                                             CCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATT                                                                                                          >  1:181040/1‑251                                                                          GGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAGGTTTCTGAACAACGCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGTAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGG                                                                                             <  1:112181/251‑1                                                                                  GTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGC                                                                                                                                                                                  <  1:57002/158‑1                                                                                     GCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAAACCTATCAATAAGTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACC                                                                                  >  1:166948/1‑251                                                                                         TCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTAGCAATAAGTTGGAGGCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTAAGCAGAATAAAGCTATTTAACCAGAAAGCGTGCTCTACTCTGAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTG                                                                                                   <  1:327179/230‑1                                                                                                        nTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTG                                                                >  1:103809/2‑250                                                                                                                     ACAAAGTCATCGGGCATTCTCTGAACATAAAACACTCTCAATAAGGTGGATTCATTACCGCTGTAATGAACATCGAATCTAACTCAGTTTCTGACCCACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTG                                                                                                                                                               >  1:298307/1‑142                                                                                                                                GGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTNTACNNNNNAG                                                                                                                                                  >  2:219285/1‑144                                                                                                                                GGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTAn                                                                                   <  1:219285/207‑2                                                                                                                                                                 GTTGGAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACGCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGGGTATTCATGTTGTGCA                                                                                               <  1:124540/162‑1                                                                                                                                                                     GAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGTAGGCGTTATGAAAACGCGTATTCATGTTGTTCAGGGTTATATTACCAAACTGGCCGTTGATG                                                                  <  1:294862/187‑1                                                                                                                                                                     GAGTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCG  >  1:4066/1‑251                                                                                                                                                                       GTCATTACCGCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACAATTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGT                                                                                           >  1:139170/1‑160                                                                                                                                                                                                                                                                                                                                                                                                                                 CGTAATCAGCAAGCGATATACGCAGCGAATTGAGCGGCATAACCTGAATCTGAGGCAGCACCTGGCACGGCTGGGACGGAAGTCGCTGTCGTTCTCAAAATCGGTGGAGCAGCATGACAAAGTCATCGGGCATTATCTGAACATAAAACACTATCAATAAGTTGGAGTCATTACC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/290808‑290634 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑GCTGTAATGAACATCGAATATAACTAAGTTTCTGAACAACTCACGTTGCTGAGCAGAAAAATGCGATTTAACCAAAAAGCCTGCTGTACACTTAAGAAACAAGAAGGTGTAAGGAGGCGTTATGAAAACGCGTATTCATGTTGTGCAGGGTGATATTACCAAACTGGCCGTTGATGTGATTGTGAATGCGGCTAATCCGTCATTAATGGGAGGCGGCGGCGTCGATGGGGCCATTCATCG  >  NC_000913/1105699‑1105938

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.