Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 4,536,236 IS5 (+) +4 bp intergenic (‑2183/‑358) topAI ← / → ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2288919 =NA (NA)11 (1.110) 8/320 0.1 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 4536236 = 0 (0.000)intergenic (‑2183/‑361) topAI/ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB
* ? NC_000913 = 2290113NA (NA)4 (0.400) 4/322 0.5 79.6% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 4536239 1 (0.100)intergenic (‑2186/‑358) topAI/ythB toxin of the TopAI‑YjhQ toxin‑antitoxin system, TopA inhibitor/protein YthB

TCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCT‑ATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCG  >  NC_000913/2288791‑2289106
                                                                                                                                |                                                                                                                                                                                            
tctcCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCCTAAAACAGCAGGATCTCCTTTTCCCAGACCACCACATCAACTCTCTCACCATACTCAACCGTTAATGACCATGCTCTTGGTTCAAATAAGGAAGGCGCGAATAAGCGGGGAAATTCTTTTCGGCTTACTCCGTCATTTTCTTTCTTCATTTTTGCGACGATTTTTTTTCCCGTTAATTCCTTGAATCAGCCTCTTTTAG‑CCGTTTCTTCGcc                                                                   >  1:95916/1‑251 (MQ=255)
                                 gTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAg                                                                                           >  1:6590/1‑195 (MQ=255)
                                                   atnnNNNTTTNCAGTACAACACCATCAACTCTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTGCGCGGCTGACTCATTCATTTCATGTCTTCATGTTTGac                                                                                                                            <  2:136103/144‑2 (MQ=25)
                                                                        ccATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCT‑ATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCg  >  1:60511/1‑244 (MQ=255)
                                                                                     cACCATACTCGACAGTTGATGCCAATNCTATGGNNGANTATCAGGAAGGTGCGAATANGCNNNGNNNTNCTTCTNGGNNGACTCAGTCNTTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCANCCT‑ATTTAGNNCGTTTCTTCGCCATTTAAGGCNTTANNCNNANtttnc                                          >  2:48540/1‑189 (MQ=17)
                                                                                                                                |                                                                                                                                                                                            
TCTCCGCCAGGATTGATTTTCGTATTATTGGCGGTCCCATAAACCAGCTGGATATCTTTTTCCAGGACAACACCATCAACTGTCTCACCATACTCAACAGTTGATGCCAATGCTATGGGTGAATATAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCT‑ATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCG  >  NC_000913/2288791‑2289106

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.