Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,682,261	Δ34 bp	coding (1154‑1187/1287 nt)	dctA ←	C4 dicarboxylate/orotate:H(+) symporter

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3682261	3682294	34	29 [0]	[0] 29	dctA	C4 dicarboxylate/orotate:H(+) symporter

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3682260	0 (0.000)	29 (1.350)	26/486	0.1	100%	coding (1188/1287 nt)	dctA	C4 dicarboxylate/orotate:H(+) symporter
	?	NC_000913	3682295 =	0 (0.000)					coding (1153/1287 nt)	dctA	C4 dicarboxylate/orotate:H(+) symporter

AGAAGTGAAATAAACCTGAACTCACCCAGGTGGTAAGTTCAGGCACAGGGGTCAATTATGCGCAAACACCCGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATG  >  NC_000913/3682053‑3682534                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                gtgttagaccgcaagaacatgaaaatgacacggaagaagtgaaataaacctgaaatcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACg                                                                                                                                                                                                                                           >  2:169398‑M2/243‑249 (MQ=255)     tagaccgcaagaacatgaaaatgtcacggaagaagtgaaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTc                                                                                                                                                                                                                                     >  1:81790‑M2/239‑251 (MQ=255)                  catgaaaatgtcacggaagaagtgaaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCgg                                                                                                                                                                                                                        <  2:62835‑M2/26‑1 (MQ=255)                  catgaaaatgtcacggaagaagtgaaataaacctgaactcacccaggcggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggcgtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtcacgttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCgg                                                                                                                                                                                                                        >  2:120873‑M2/226‑251 (MQ=255)                             cacggaagaagtgaaataaacctgaactcacccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCg                                                                                                                                                                                                               >  1:66544‑M2/215‑249 (MQ=255)                                                  ctgaactcacccaggtggtaagtgcaggcacaggggtcaattatgcgcacacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtgctccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAACGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTAc                                                                                                                                                                                        >  2:90980‑M2/194‑251 (MQ=255)                                                            ccaggtggtaagttcaggcacaggggtcaattatgcgcaaacacccgaactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAANTgg                                                                                                                                                                              >  2:201717‑M2/184‑251 (MQ=255)                                                                     aagttcaggcacaggggtcaattatgctcaaacccccgcactcggggaagggagtgcgggcataagtgatgagatttagtggatacttcgtgcgttttgccatccgtcgcacgattattcagcacatcgtccattttgttgtggtccagtgctttcactcacttagcaacgacaGCGCACGAGCTTCTGTCGTAAAGCGTTCGGTACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCTACCGCa                                                                                                                                                                     <  1:26595‑M2/77‑1 (MQ=255)                                                                          caggcacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggctcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCagagag                                                                                                                                                                <  1:168622‑M2/82‑1 (MQ=255)                                                                              cacaggggtcaattatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTc                                                                                                                                                            >  1:151156‑M2/166‑251 (MQ=255)                                                                                     gtcaattatgcgcaaacgcccgcactcggggaagggagtgcgtgcataagtgatgagattaagaggattattcgttcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGATCGTcgccgccc                                                                                                                                                     <  2:160428‑M2/93‑2 (MQ=255)                                                                                      tcacttatgcgcaaacacccgcactcggggaagggagtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgtacgattattcagcacatcgtccagttttttgtggtccagttctttaacccacttagcaccgacaGCGCACGAGCTTCTGACATAAAGCGGCCGATACCGAGGATCAGCGCCAGACCCGCTACCGTCAAATGGCCCACCGCAGAGAGCGTCgccgcc                                                                                                                                                      >  2:65750‑M2/158‑249 (MQ=255)                                                                                                            actcggggaagggcgtgcgggcataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtcgagttttttgtggtacagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACAGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCTCTACCCGtt                                                                                                                              >  2:96204‑M2/136‑251 (MQ=255)                                                                                                                                  ataagtgatgagattaagaggataattcgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTaga                                                                                                          >  1:103701‑M2/114‑249 (MQ=255)                                                                                                                                                         aattcgttcttttggccatccggcgcacgagtattcagcacatcgtccagttttttttggtccagttctttcacccccttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAg                                                                                  <  2:177468‑M2/160‑1 (MQ=255)                                                                                                                                                             cgtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTg                                                                              >  1:49904‑M2/87‑250 (MQ=255)                                                                                                                                                              gtgcgttttgccatccggcgcacgattattcagcacatcgtccagttttttgtgatccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTCCCGTCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGAt                                                                            <  2:39982‑M2/166‑1 (MQ=255)                                                                                                                                                                       gccatccggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGAACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGAc                                                                   <  1:36283‑M2/175‑1 (MQ=255)                                                                                                                                                                             cggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATc                                                             >  2:7309‑M2/71‑251 (MQ=255)                                                                                                                                                                              ggcgcacgattattcagcacatcgtccagttttttgtggtccagttctttcacccacgtagcaacgccaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATcc                                                            <  1:90980‑M2/182‑1 (MQ=255)                                                                                                                                                                               gcgcacgattattcagcccatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGTCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCa                                                           <  1:120873‑M2/183‑1 (MQ=255)                                                                                                                                                                                         attcagcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTACCAACGTGCTTTGCGGGACGATatccatcttcctgtt                                                 >  2:46809‑M2/59‑244 (MQ=255)                                                                                                                                                                                         attcagcacatcgtccagttttttgtggcccagttctttcacccaattaacaacgacaGCGTACGATCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGCGCGTCGCCGCCAGCACAATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGACGAAAGCAGCAACACACTTATCAGCGTTATTTGGTGGCcgatatccatatagatgtt                                                 >  1:27130‑M2/59‑242 (MQ=255)                                                                                                                                                                                             agcacatcgtccagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTg                                             <  1:201609‑M2/197‑1 (MQ=255)                                                                                                                                                                                                        cagttttttgtggtccagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGATCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCACCACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGAt                                  <  1:427‑M2/208‑1 (MQ=255)                                                                                                                                                                                                                       cagttctttcacccacttagcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGCTTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGTCCTGGTCGATAAACACCGCCGCCAt                   >  2:42587‑M2/29‑251 (MQ=255)                                                                                                                                                                                                                                    cccttagcaacgacgGCCCACGAGCTTCTGACATAAAGCGGTCGATCCCGAGGATCAGCGCGAGACCCGCTTCCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCATCACGATAAAGCCTCTACCCGTTCCCCCTGCCGCCCCTTTAAAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATGTCCATCGGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATc      <  1:96204‑M2/236‑1 (MQ=255)                                                                                                                                                                                                                                      ataagaaacgacaGCTCACGAGCTTCTGACAGAAAGCGGTCGATACCGGGGATCAGCGCCAACCCCGCTCCCGCCAAATGCCCCACCGCAGAGGGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTGAGAAGAAAGCAGCAACACGATTAACAGCGTGATTGGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGa    <  2:103701‑M2/238‑1 (MQ=255)                                                                                                                                                                                                                                         agcaacgacaGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGAtg  <  1:193966‑M2/240‑1 (MQ=255)                                                                                                                                                                                                                                                   |                                                                                                                                                                                                                                                AGAAGTGAAATAAACCTGAACTCACCCAGGTGGTAAGTTCAGGCACAGGGGTCAATTATGCGCAAACACCCGCACTCGGGGAAGGGAGTGCGGGCATAAGTGATGAGATTAAGAGGATAATTCGTGCGTTTTGCCATCCGGCGCACGATTATTCAGCACATCGTCCAGTTTTTTGTGGTCCAGTTCTTTCACCCACTTAGCAACGACAATGGTCGCTACGCCGTTACCGACCAGGTTAGTCAGCGCACGAGCTTCTGACATAAAGCGGTCGATACCGAGGATCAGCGCCAGACCCGCTACCGGCAAATGGCCCACCGCAGAGAGCGTCGCCGCCAGCACGATAAAGCCACTACCCGTTACCCCTGCCGCCCCTTTAGAAGAAAGCAGCAACACGATTAACAGCGTGATTTGGTGGACGATATCCATCTGACTGTTAGTGGCCTGGGCGATAAACACCGCCGCCATTGTCAGGTATATCGATG  >  NC_000913/3682053‑3682534

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑