Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,077,568	Δ1,832 bp		[cmtA]–cmtB	[cmtA], cmtB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3077568	3079399	1832	38 [0]	[0] 38	[cmtA]–cmtB	[cmtA],cmtB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3077567	0 (0.000)	38 (0.620)	35/490	0.3	100%	coding (1293/1389 nt)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
	?	NC_000913	3079400 =	0 (0.000)					intergenic (‑70/+244)	cmtB/tktA	mannitol‑specific PTS enzyme IIA component CmtB/transketolase 1

TAATGGTGGAATCTTTAATTGCCTGAATGTAATTCTCGCTGATATAGTTTTTATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATT  >  NC_000913/3079177‑3079645                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       cgctgtcagaaattacactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCCATTGAGCAGGGAAACCGGTGtt‑t                                                                                                                                                                                                                            >  1:270669‑M2/224‑251 (MQ=255)    tgtcagaaattacactccccagtaattcattatcggtaatttctgccagttcaaatacgcgcagacggaaatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcgggtaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACAGGTGTT‑TTg                                                                                                                                                                                                                          >  2:153902‑M2/221‑250 (MQ=255)               acactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAat                                                                                                                                                                                                              >  1:262863‑M2/210‑251 (MQ=255)                cactcaccagtaattcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggattgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAatt                                                                                                                                                                                                             >  2:103401‑M2/209‑251 (MQ=255)                            attcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGGAAACAGTGAATTATTAATTTCtt                                                                                                                                                                                                  >  1:387549‑M2/197‑250 (MQ=255)                            attcattatcggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGCAAACAGTGAATTATTAATTTCtt                                                                                                                                                                                                  >  1:366606‑M2/197‑250 (MQ=255)                               cattatcggtatgttctggcagttcaaatgcgcgcagacggacatccctcttgccatcaatagcagtaactttggttttcatttttgcctctgtaatcaggggttatgttcggtggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatctatatgagtggtttatccgtcacaAATCAAGTGAGCATGGAAACCTGTGTT‑TTGCAAACAGTGAATTATTAATTTCTtata                                                                                                                                                                                               <  2:137148‑M2/57‑1 (MQ=255)                                     cggtaatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACAtt                                                                                                                                                                                        <  1:238203‑M2/64‑1 (MQ=255)                                         aatttctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAg                                                                                                                                                                                     <  1:61725‑M2/67‑1 (MQ=255)                                              ctggcagttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattactcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTtat                                                                                                                                                                                 >  2:123721‑M2/179‑249 (MQ=255)                                                   agttcaaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagcgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGatat                                                                                                                                                                           >  1:139521‑M2/174‑250 (MQ=255)                                                        aaatacgcgcagacggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAATCAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATaacaac                                                                                                                                                                     <  1:273264‑M2/83‑1 (MQ=255)                                                            acgcgcagacggacatcccgcttgccataaatagcagcatctttggttttcagttttacctctgtaatcagtgtttatgttcggctgttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatagcaggggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTGATATAACATTGAGTTATAGATATAACAGCAAAt                                                                                                                                                                 <  2:371989‑M2/87‑1 (MQ=255)                                                                      ggacatcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGc                                                                                                                                                       >  2:31268‑M2/155‑251 (MQ=255)                                                                          atcccgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGGGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCatat                                                                                                                                                   >  2:83396‑M2/151‑251 (MQ=255)                                                                          atcccgcttgccataaatagcagcaactttgggtttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgaggtttggatcgccaatatagtttttaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCATGGAAACCGGTGGT‑TTGAAAAAGGTGAATTATTAATTTCTTATTTAACATTGAGTTATAGATATAAAAACAAATGTCAATACGCata                                                                                                                                                    >  2:411116‑M2/151‑250 (MQ=255)                                                                              cgcttgccataaatagcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTAAATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTg                                                                                                                                               <  1:263496‑M2/105‑1 (MQ=255)                                                                                            agcagcaactttggttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACAt                                                                                                                                  >  1:86846‑M2/133‑250 (MQ=255)                                                                                                          ttttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAAAAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGatatttattt                                                                                                                   >  2:434185‑M2/119‑250 (MQ=255)                                                                                                           tttcattttttactctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTg                                                                                                                   <  2:260780‑M2/133‑1 (MQ=255)                                                                                                           tttcatttttacctctgtaatcagtgtttatgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGc                                                                                                                  >  1:97547‑M2/118‑251 (MQ=255)                                                                                                                                        atgttcggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTACTTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGAATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGcc                                                                                     >  2:119337‑M2/89‑251 (MQ=255)                                                                                                                                             cggcggttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTTATATTTATTGCATATAAATATTTGTGTTCTACAACACGCCTTATc                                                                                <  2:403066‑M2/168‑1 (MQ=255)                                                                                                                                                   ttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGata                                                                                                                          >  1:86162‑M2/78‑203 (MQ=255)                                                                                                                                                   ttaattgattaaataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGata                                                                                                                          <  2:86162‑M2/126‑1 (MQ=255)                                                                                                                                                               ataaagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAAc                                                              <  2:9305‑M2/186‑1 (MQ=255)                                                                                                                                                                  aagtgtcgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACgg                                                            >  2:84596‑M2/63‑250 (MQ=255)                                                                                                                                                                        cgagttttggatcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACatat                                                      <  1:93535‑M2/194‑1 (MQ=255)                                                                                                                                                                                  atcgccaatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCACGGtaata                                            >  1:2224‑M2/47‑250 (MQ=255)                                                                                                                                                                                        aatatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAgg                                     <  1:411116‑M2/211‑1 (MQ=255)                                                                                                                                                                                         atatagttattaatcaatatcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGt                                    >  2:32641‑M2/40‑251 (MQ=255)                                                                                                                                                                                                           atcagtggtttagccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCtttt                   <  2:51784‑M2/229‑1 (MQ=255)                                                                                                                                                                                                            tcagtggtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCttttt                  >  2:361641‑M2/21‑250 (MQ=255)                                                                                                                                                                                                                  gtttatccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGa            >  1:312177‑M2/15‑250 (MQ=255)                                                                                                                                                                                                                    atatccgtcacaAAGAAATTGCGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTCTTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGCAGCGACCTTTTTACCCGAAAt         >  2:71732‑M2/13‑251 (MQ=255)                                                                                                                                                                                                                        ccgtcacaAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCt      >  2:301053‑M2/9‑250 (MQ=255)                                                                                                                                                                                                                        ccgtcacaAAGCAATTGAGCAGGGAAACCGG‑GTTCTTGAAATCAGTGAATTAGTAATTTCTTATATAACATTGATTTATAGATATAACAACAAATGTCAATGCTCAACTCGTGATGCATATTACAAAATTGATAGTTATTGCATATAAAAATTTGTGAACTACAACACGCCTTATCTATTGCTGGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAATCGACCTGTTTACCAGAAATGCt      <  2:294268‑M2/242‑1 (MQ=255)                                                                                                                                                                                                                            cacaAAGCAATTGAGCAGGGAAACCGGTGAT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCAAAtt  >  2:386347‑M2/5‑250 (MQ=255)                                                                                                                                                                                                                                |                                                                                                                                                                                                                                                       TAATGGTGGAATCTTTAATTGCCTGAATGTAATTCTCGCTGATATAGTTTTTATCCAGCAATGATACCATCGAGAAATCGATAGCTTCCTGCCAATCTTTTGCTGAATGTATAACTGAGATTGATGATTCTGGAAAATAATCACTAAGCCGCATAAATTATCCTTATTTTATTTTTAGGGTACCGTCCGGCGGTAAAAATAAAATACTCGTGCGGTTTAATGAAAGCAATTGAGCAGGGAAACCGGTGTT‑TTGAAAACAGTGAATTATTAATTTCTTATATAACATTGAGTTATAGATATAACAACAAATGTCAATACGCATATCGTGATGCATATTACATAATTGATATTTATTGCATATAAATATTTGTGATCTACAACACGCCTTATCTATTGCTTGTCCGCAAACGGACATATCAAGGTAATAAAAAAGGTCGCCGAAGCGACCTTTTTACCCGAAATGCTAATT  >  NC_000913/3079177‑3079645

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑