Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	3,077,568	Δ1,832 bp		[cmtA]–cmtB	[cmtA], cmtB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	3077568	3079399	1832	38 [0]	[0] 38	[cmtA]–cmtB	[cmtA],cmtB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 3077567	0 (0.000)	38 (0.620)	35/490	0.3	100%	coding (1293/1389 nt)	cmtA	mannitol‑specific PTS enzyme IICB component CmtA
	?	NC_000913	3079400 =	0 (0.000)					intergenic (‑70/+244)	cmtB/tktA	mannitol‑specific PTS enzyme IIA component CmtB/transketolase 1

CGCTGTCAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACACGTTTCACGCGCCCTTCCAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGCCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGCAAACAAAGGCGATACGCTTAACCCTGCTTAACGAGAATGCACCCTCTTGTTTCATCGCCTTAACCGCATTGGCTGACTGAGCA  >  NC_000913/3077345‑3077813                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       cGCTGTCAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagccattgagcagggaaaccggtgttt                                                                                                                                                                                                                            >  1:270669‑M1/1‑223 (MQ=255)    tGTCAGAAATTACACTCCCCAGTAATTCATTATCGGTAATTTCTGCCAGTTCAAATACGCGCAGACGGAAATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGGTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaacaggtgttttg                                                                                                                                                                                                                          >  2:153902‑M1/1‑220 (MQ=255)               acacTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaat                                                                                                                                                                                                              >  1:262863‑M1/1‑209 (MQ=255)                cacTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATTGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaatt                                                                                                                                                                                                             >  2:103401‑M1/1‑208 (MQ=255)                            aTTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttggaaacagtgaattattaatttctt                                                                                                                                                                                                  >  1:387549‑M1/1‑196 (MQ=255)                            aTTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgcaaacagtgaattattaatttctt                                                                                                                                                                                                  >  1:366606‑M1/1‑196 (MQ=255)                               cATTATCGGTATGTTCTGGCAGTTCAAATGCGCGCAGACGGACATCCCTCTTGCCATCAATAGCAGTAACTTTGGTTTTCATTTTTGCCTCTGTAATCAGGGGTTATGTTCGGTGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCTATATGAGTGGTTTATCCGTCACAaatcaagtgagcatggaaacctgtgttttgcaaacagtgaattattaatttcttata                                                                                                                                                                                               <  2:137148‑M1/250‑58 (MQ=255)                                     cGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacatt                                                                                                                                                                                        <  1:238203‑M1/251‑65 (MQ=255)                                         aaTTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgag                                                                                                                                                                                     <  1:61725‑M1/250‑68 (MQ=255)                                              cTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTACTCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttat                                                                                                                                                                                 >  2:123721‑M1/1‑178 (MQ=255)                                                   aGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGCGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatat                                                                                                                                                                           >  1:139521‑M1/1‑173 (MQ=255)                                                        aaaTACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaatcagtgaattattaatttcttatataacattgagttatagatataacaac                                                                                                                                                                     <  1:273264‑M1/251‑84 (MQ=255)                                                            aCGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCATCTTTGGTTTTCAGTTTTACCTCTGTAATCAGTGTTTATGTTCGGCTGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATAGCAGGGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttctgatataacattgagttatagatataacagcaaat                                                                                                                                                                 <  2:371989‑M1/251‑88 (MQ=255)                                                                      ggACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgc                                                                                                                                                       >  2:31268‑M1/1‑154 (MQ=255)                                                                          aTCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgggcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatat                                                                                                                                                   >  2:83396‑M1/1‑150 (MQ=255)                                                                          aTCCCGCTTGCCATAAATAGCAGCAACTTTGGGTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGGTTTGGATCGCCAATATAGTTTTTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcatggaaaccggtggtttgaaaaaggtgaattattaatttcttatttaacattgagttatagatataaaaacaaatgtcaatacgcata                                                                                                                                                    >  2:411116‑M1/1‑150 (MQ=255)                                                                              cGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttaaataacattgagttatagatataacaacaaatgtcaatacgcatatcgtg                                                                                                                                               <  1:263496‑M1/251‑106 (MQ=255)                                                                                            agcagcAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacat                                                                                                                                  >  1:86846‑M1/1‑132 (MQ=255)                                                                                                          ttttCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataaaaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattt                                                                                                                   >  2:434185‑M1/1‑118 (MQ=255)                                                                                                           tttCATTTTTTACTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattg                                                                                                                   <  2:260780‑M1/250‑134 (MQ=255)                                                                                                           tttCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgc                                                                                                                  >  1:97547‑M1/1‑117 (MQ=255)                                                                                                                                        aTGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattactttcttatataacattgagttatagatataacaacaaatgtcaatacgaatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgcc                                                                                     >  2:119337‑M1/1‑88 (MQ=255)                                                                                                                                             cggcggTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataatttatatttattgcatataaatatttgtgttctacaacacgccttatc                                                                                <  2:403066‑M1/251‑169 (MQ=255)                                                                                                                                                   ttAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgata                                                                                                                          >  1:86162‑M1/1‑77 (MQ=255)                                                                                                                                                   ttAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgata                                                                                                                          <  2:86162‑M1/203‑127 (MQ=255)                                                                                                                                                               ataaaGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaac                                                              <  2:9305‑M1/251‑187 (MQ=255)                                                                                                                                                                  aaGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacgg                                                            >  2:84596‑M1/1‑62 (MQ=255)                                                                                                                                                                        cGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatat                                                      <  1:93535‑M1/250‑195 (MQ=255)                                                                                                                                                                                  aTCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcacggtaata                                            >  1:2224‑M1/1‑46 (MQ=255)                                                                                                                                                                                        aaTATAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaagg                                     <  1:411116‑M1/251‑212 (MQ=255)                                                                                                                                                                                         atatAGTTATTAATCAATATCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggt                                    >  2:32641‑M1/1‑39 (MQ=255)                                                                                                                                                                                                           atCAGTGGTTTAGCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgacctttt                   <  2:51784‑M1/250‑230 (MQ=255)                                                                                                                                                                                                            tCAGTGGTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgaccttttt                  >  2:361641‑M1/1‑20 (MQ=255)                                                                                                                                                                                                                  gTTTATCCGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgacctttttacccga            >  1:312177‑M1/1‑14 (MQ=255)                                                                                                                                                                                                                    ataTCCGTCACAaagaaattgcgcagggaaaccggtgttttgaaaacagtgaattcttaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgcagcgacctttttacccgaaat         >  2:71732‑M1/2‑12 (MQ=255)                                                                                                                                                                                                                        ccGTCACAaagcaattgagcagggaaaccggtgttttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgacctttttacccgaaatgct      >  2:301053‑M1/1‑8 (MQ=255)                                                                                                                                                                                                                        ccGTCACAaagcaattgagcagggaaaccgggttcttgaaatcagtgaattagtaatttcttatataacattgatttatagatataacaacaaatgtcaatgctcaactcgtgatgcatattacaaaattgatagttattgcatataaaaatttgtgaactacaacacgccttatctattgctggtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaatcgacctgtttaccagaaatgct      <  2:294268‑M1/250‑243 (MQ=255)                                                                                                                                                                                                                            cacaaagcaattgagcagggaaaccggtgatttgaaaacagtgaattattaatttcttatataacattgagttatagatataacaacaaatgtcaatacgcatatcgtgatgcatattacataattgatatttattgcatataaatatttgtgatctacaacacgccttatctattgcttgtccgcaaacggacatatcaaggtaataaaaaaggtcgccgaagcgacctttttacccgaaatgcaaatt  >  2:386347‑M1/1‑4 (MQ=255)                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                       CGCTGTCAGAAATTACACTCACCAGTAATTCATTATCGGTAATTTCTGGCAGTTCAAATACGCGCAGACGGACATCCCGCTTGCCATAAATAGCAGCAACTTTGGTTTTCATTTTTACCTCTGTAATCAGTGTTTATGTTCGGCGGTTAATTGATTAAATAAAGTGTCGAGTTTTGGATCGCCAATATAGTTATTAATCAATATCAGTGGTTTATCCGTCACACGTTTCACGCGCCCTTCCAGACTGGCATGAGTAACGACGATATCCGCATCCGCAGGCACGTTTTCTATGGCGTAATGTTTTACTTCAATTGCCAGCCCCGCTTTTTCCAGGCGTTTACGGAAGGTGGTCGCGCCCATCGCACTGGAGCCCATCCCCGCATCGCAAACAAAGGCGATACGCTTAACCCTGCTTAACGAGAATGCACCCTCTTGTTTCATCGCCTTAACCGCATTGGCTGACTGAGCA  >  NC_000913/3077345‑3077813

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑