| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 2,498,768 | 9486 bp→CCC | [pyrS]–fryB | [pyrS], pyrR, ypdC, fryA, ypdE, ypdF, fryC, fryB | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 2498768 | 2508253 | 9486 | 29 [0] | [0] 28 | [pyrS]–fryB | [pyrS],pyrR,ypdC,fryA,ypdE,ypdF,fryC,fryB |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 2498767 | 0 (0.000) | 28 (0.460) +CCC |
26/488 | 0.7 | 100% | coding (97/1698 nt) | pyrS | sensor histidine kinase PyrS |
| ? | NC_000913 | 2508254 = | 0 (0.000) | intergenic (‑12/+207) | fryB/glk | putative PTS enzyme IIB component FryB/glucokinase | |||||
GGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGGATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGGCTTCTACCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGC > NC_000913/2508038‑2508496 | caccatttatctatcaatccccttttaagattgtggtttttccgcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttatgcagagtcgcccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcagccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACg < 1:131651‑M2/35‑1 (MQ=255) atttatctatcaatccccttttaaaattgtggtttttcctcacaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcctccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTTTGAACGTGc > 2:110082‑M2/213‑250 (MQ=255) ggtttttcctcaaaaacgtcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttttcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATg < 2:301021‑M2/69‑1 (MQ=255) tttttcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAACAGACAAATAATGc > 1:47089‑M2/181‑250 (MQ=255) tcctcaaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCAttt > 1:41288‑M2/177‑250 (MQ=255) aaaaacggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACtggg > 1:415304‑M2/172‑250 (MQ=255) acggcctgctggtcatcagccttcaggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAt < 2:280448‑M2/84‑1 (MQ=255) ggttacctatcatagaggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGAc > 1:16554‑M2/143‑251 (MQ=255) aggtttaatccttattcagagtcacccgtgcacgaaatattcaacatgctgctgtcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTtgt < 1:496426‑M2/124‑1 (MQ=255) aatccttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAggg < 1:110082‑M2/129‑1 (MQ=255) ttattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGGCGCAAGTCGACCTTgtgaggggcaac > 1:263836‑M2/117‑246 (MQ=255) tattcagagtcacccgtgcacgaaatattccacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGa > 1:359942‑M2/116‑125 (MQ=255) tattcagagtcacccgtgcacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGacac < 1:161861‑M2/134‑1 (MQ=255) tattcagaggcacccgtgcacgaaagattcaacatgctgctggcggtcttcgatcgggcagcgtttaggcttatctgcctgttctttctcatccgtatccgccggtttcgcgcccCGTTCAGTGa < 2:359942‑M2/10‑1 (MQ=255) ccggagcctcccgtgcagtaaaacttcaacaggctgctgtcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccggctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGATCGTGCAAAACAAATCGCCGTTACCATACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGTCGCAGGTCGCCCTTGTGAGTGGACACAAAGa < 2:349834‑M2/139‑1 (MQ=255) cagagtcacccgtgcacgaaatattcaacaggctgctggcggtcttctatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgttccgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGaa < 2:331826‑M2/140‑1 (MQ=255) cacgaaatattcaacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACt > 2:365540‑M2/98‑251 (MQ=255) caacatgctgctggcggtcttcgatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTa < 1:152641‑M2/164‑1 (MQ=255) acatgctgctggcggtcttcgatcggacagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTaaaa < 1:92524‑M2/167‑1 (MQ=255) catgctgctggcgggctcagatcgggcagcgatatgtcttatcgtcctgtcctttctcagccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCAGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTtaaaaa < 2:18872‑M2/168‑2 (MQ=255) tggcggtcttcgatcgggcagcgttaatgcttatcaggctgttctttctcatccgtatccgcctgtttcgcgcacCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAACACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCAGCTGTACGGAAGTGTGAAGCAGGTCGACCTTGTGAGGGGCCACAAAGAAAAATTGAATAAAATGTATGATTTAAAAGATTATCgg > 2:451586‑M2/76‑251 (MQ=255) gatcgggcagcgttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAACAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCt > 2:208572‑M2/65‑251 (MQ=255) gttaatgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATaaa < 1:292885‑M2/198‑1 (MQ=255) atgcttatctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGCGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGCGTTACCTCCCGATATAAaagg > 2:297194‑M2/50‑250 (MQ=255) atctgcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAACAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGAtt > 1:122649‑M2/44‑251 (MQ=255) gcctgttctttctcatccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGGATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTAc > 1:187484‑M2/40‑250 (MQ=255) ccgtatccgcctgtttcgcgcccCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGt > 1:402601‑M2/24‑251 (MQ=255) cgcgcccCGGTCAGGGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGc < 2:16554‑M2/243‑1 (MQ=255) | GGGTAACTGCCACGGAGTGGATGATGATGGTCGCTTCGGCGATATCCTGCGCCGTCAGGCGATTCTGGATACCGTCCGCGCCCTGGGTTTCAATTTTCACTTCATAACCGGCTTCTACCGCCGCTTCTTCCAGCGCCTGAGCGGCCATAAAGGTGTGAGCCAGGCCCATCGGGCAGGCACATAAGGCAATCAGTTTCTTACTCATCGTTAATTCCTCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGC > NC_000913/2508038‑2508496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |