| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| MC JC | NC_000913 | 2,498,768 | 9486 bp→CCC | [pyrS]–fryB | [pyrS], pyrR, ypdC, fryA, ypdE, ypdF, fryC, fryB | |
| Missing coverage evidence... | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|
| seq id | start | end | size | ←reads | reads→ | gene | description | |||
| * | * | ÷ | NC_000913 | 2498768 | 2508253 | 9486 | 29 [0] | [0] 28 | [pyrS]–fryB | [pyrS],pyrR,ypdC,fryA,ypdE,ypdF,fryC,fryB |
| New junction evidence | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | reads (cov) | reads (cov) | score | skew | freq | annotation | gene | product | ||
| * | ? | NC_000913 | = 2498767 | 0 (0.000) | 28 (0.460) +CCC |
26/488 | 0.7 | 100% | coding (97/1698 nt) | pyrS | sensor histidine kinase PyrS |
| ? | NC_000913 | 2508254 = | 0 (0.000) | intergenic (‑12/+207) | fryB/glk | putative PTS enzyme IIB component FryB/glucokinase | |||||
AACCAGAAGTGCATTATCAAGAGATGCATTGCTAATACACCATTTATCTATCAATCCCCTTTTAAAATTGTGGTTTTTCCTCAAAAACGGCCTGCTGGTCATCAGCCTTCAGGTTACCTATCATAGAGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913/2498518‑2498767‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGC > NC_000913/2508254‑2508496 ||| AACCAGAAGTGCATTATCAAGAGATGCATTGCTAATACACCATTTATCTATCAATCCCCTTTTAAAATTGTGGTTTTTCCTCAAAAACGGCCTGCTGGTCATCAGCCTTCAGGTTACCTATCATAGAGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCCTCCGTATCCGCCTGTTTCGCGC > 2:152641/1‑251 CACCATTTATCTATCAATCCCCTTTTAAGATTGTGGTTTTTCCGCAAAAACGGCCTGCTGGTCATCAGCCTTCAGGTTACCTATCATAGAGGTTTAATCCTTATGCAGAGTCGCCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCAGCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACG < 1:131651/251‑1 ATTTATCTATCAATCCCCTTTTAAAATTGTGGTTTTTCCTCACAAACGGCCTGCTGGTCATCAGCCTTCAGGTTACCTATCATAGAGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCCTCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTTTGAACGTGC > 2:110082/1‑250 GGTTTTTCCTCAAAAACGTCCTGCTGGTCATCAGCCTTCAGGTTACCTATCATAGAGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTTTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATG < 2:301021/251‑1 TTTTTCCTCAAAAACGGCCTGCTGGTCATCAGCCTTCAGGTTACCTATCATAGAGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAACAGACAAATAATGC > 1:47089/1‑250 TCCTCAAAAACGGCCTGCTGGTCATCAGCCTTCAGGTTACCTATCATAGAGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTT > 1:41288/1‑250 AAAAACGGCCTGCTGGTCATCAGCCTTCAGGTTACCTATCATAGAGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGg > 1:415304/1‑250 ACGGCCTGCTGGTCATCAGCCTTCAGGTTACCTATCATAGAGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAAT < 2:280448/251‑1 GGTTACCTATCATAGAGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGAC > 1:16554/1‑251 AGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGTCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGT < 1:496426/251‑1 AATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGG < 1:110082/250‑1 TTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGGCGCAAGTCGACCTTGTGAGGGGcaac > 1:263836/1‑246 TATTCAGAGTCACCCGTGCACGAAATATTCCACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGA > 1:359942/1‑125 TATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACAC < 1:161861/249‑1 TATTCAGAGGCACCCGTGCACGAAAGATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTTAGG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCGGTTTCGCGCCCCGTTCAGTGA < 2:359942/125‑1 ccggagcctcccgtgcagtaaaacTTCAACAGGCTGCTGTCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGGCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGATCGTGCAAAACAAATCGCCGTTACCATACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGTCGCAGGTCGCCCTTGTGAGTGGACACAAAGA < 2:349834/227‑1 CAGAGTCACCCGTGCACGAAATATTCAACAGGCTGCTGGCGGTCTTCTATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTCCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAA < 2:331826/251‑1 CACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACT > 2:365540/1‑251 CAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTA < 1:152641/250‑1 ACATGCTGCTGGCGGTCTTCGATCGGACAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAA < 1:92524/251‑1 CATGCTGCTGGCGGGCTCAGATCGGGCAGCGATA‑TGTCTTATCGTCCTGTCCTTTCTCAGCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCAGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAa < 2:18872/251‑2 TGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCAGGCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCACCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAACACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCAGCTGTACGGAAGTGTGAAGCAGGTCGACCTTGTGAGGGGCCACAAAGAAAAATTGAATAAAATGTATGATTTAAAAGATTATCGG > 2:451586/1‑251 GATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAACAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCT > 2:208572/1‑251 GTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAA < 1:292885/251‑1 ATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGCGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGCGTTACCTCCCGATATAAAAGG > 2:297194/1‑250 ATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTAACAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATT > 1:122649/1‑251 GCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGGATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTAC > 1:187484/1‑250 CCGTATCCGCCTGTTTCGCGCCCCGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGT > 1:402601/1‑251 CGCGCCCCGGTCAGGGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGC < 2:16554/250‑1 ||| AACCAGAAGTGCATTATCAAGAGATGCATTGCTAATACACCATTTATCTATCAATCCCCTTTTAAAATTGTGGTTTTTCCTCAAAAACGGCCTGCTGGTCATCAGCCTTCAGGTTACCTATCATAGAGGTTTAATCCTTATTCAGAGTCACCCGTGCACGAAATATTCAACATGCTGCTGGCGGTCTTCGATCGGGCAGCGTTAATG‑CTTATCTGCCTGTTCTTTCTCATCCGTATCCGCCTGTTTCGCG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑ > NC_000913/2498518‑2498767‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑CGTTCAGTGATAGTTGACGAGTGAAATTATGAACGTGCAAAACAAATCGCCGTTACCAGACAAATAATGCATTTACTGGAGAATTAATCCCGCTGTACGGAAGTGTGACGCAGGTCGACCTTGTGAGGGGACACAAAGAAAAATTGAATAAACTGTATGATTTAAAAGATTATCGGGAGAGTTACCTCCCGATATAAAAGGAAGGATTTACAGAATGTGACCTAAGGTCTGGCGTAAATGTGC > NC_000913/2508254‑2508496 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |
| Reads not counted as support for junction |
| read_name Not counted due to insufficient overlap past the breakpoint. |
| read_name Not counted due to not crossing MOB target site duplication. |