Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,223,636 IS2 (–) +5 bp intergenic (+187/+639) ymgJ → / ← minE uncharacterized protein YmgJ/Z‑ring positioning protein MinE

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1223636 =4 (0.160)24 (0.940) 20/486 0.3 85.5% intergenic (+187/+643) ymgJ/minE uncharacterized protein YmgJ/Z‑ring positioning protein MinE
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 12236404 (0.160)22 (0.860) 20/484 0.3 84.3% intergenic (+191/+639) ymgJ/minE uncharacterized protein YmgJ/Z‑ring positioning protein MinE
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

GCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTC  >  NC_000913/1467671‑1468152
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  
gcTTGCAGATGGGTAGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGcccc                                                                                                                                                                                                                                         <  2:97202/251‑1 (MQ=255)
       gATGGGTCGCATGTTACTTCCTTGCGCACAGGATATAGTTATTCCAGCGTTATTGGCTCTAGTTGTGAGTCGTCGGTATATCTGAATGGAGATACTCCAATCAGCGGAGAATTCCCTCTGGGTTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACTCTAACAATGCAGGATATTGACTGTTTTGAACATCATGTGACAAGAACTCTGTATTTGCCCCTATAttt                                                                                                                                                                                                                                  <  1:180670/251‑1 (MQ=255)
               gCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACAt                                                                                                                                                                                                                          >  1:291607/1‑251 (MQ=255)
                            ttGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGCCAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACtt                                                                                                                                                                                                              >  2:88445/1‑250 (MQ=255)
                                    aGGAGATAGTTATACCATCGGTATTGTCTTTAGGTGTTATTCGTCGTTATATCTGAAGTGAGATACGACAATCAGCGTAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCATGATATTGACAGGTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTa                                                                                                                                                                                                     <  2:270590/251‑1 (MQ=255)
                                       atatAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAAAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACa                                                                                                                                                                                                   >  2:232512/1‑250 (MQ=255)
                                                             gTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTccc                                                                                                                                                                            >  1:7000/1‑251 (MQ=255)
                                                                           gtcgtcGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAACTTGCAGTGGCGCTACGCTAACAATGAAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATaa                                                                                                                                                              <  2:96802/251‑1 (MQ=255)
                                                                                  gtacatCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAgcg                                                                                                                                                       <  1:65486/247‑1 (MQ=255)
                                                                                          gAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGc                                                                                                                                               <  1:160753/251‑1 (MQ=255)
                                                                                                 gatatgaaaaTCAGCGGCGAATTTCCTCTAGTGATTGTCGGGGTTATTCGGTGACAGGATAAAGTGTTGCGTGAAATTGGCAGTGGCGCTACGCTAACAATGCATGATATTGACAGTTTTGAACATCATGGGACAAGAACCTTGGATTTGCCCCGATAATGCCAGACATCTGTTATCACTTAACCCATGACAAGCCCGCTGCCGCAGATATGCCCGTGGCTAGCGATAACCCAGCGCACTATGCGGATGc                                                                                                                                         <  2:131573/242‑1 (MQ=18)
                                                                                                          aTCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTtat                                                                                                                               <  1:88445/251‑1 (MQ=255)
                                                                                                              gCGGAGAATTCCCTCTGGGGCTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATGTCCAGACATCTGTTATCACTTAACCCATTACAAGCGCGCTGCCGCAGATATTCCCGTGGCGAGCGATAGCCCAGCGCACTATGCGGATGCCATTCGTTATAATg                                                                                                                           <  1:25039/251‑1 (MQ=255)
                                                                                                                         ccTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGccc                                                                                                                >  1:121088/1‑250 (MQ=255)
                                                                                                                                 gTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACATAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAgg                                                                                                        <  1:122709/251‑1 (MQ=255)
                                                                                                                                                                     gCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATg                                                                    >  1:42179/1‑251 (MQ=255)
                                                                                                                                                                            aTTGGCAGTGGCGCTACGCTAACAATGCAGGATATTTACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGGTATCACTTAACCCATTACAAGCCCGCTGCCCCAGATATTCCCGTGGCGAGCGATAACCCAGACCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCCCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGGTACTg                                                              >  2:152205/1‑250 (MQ=18)
                                                                                                                                                                                gCAGCGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACCTCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCAGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCACGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATCCtgttgt                                                          >  2:175700/1‑250 (MQ=255)
                                                                                                                                                                                          tacgctaACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCACTTCGTTATAATGCTCGAACGACTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCttt                                               >  2:161229/1‑251 (MQ=255)
                                                                                                                                                                                              ctaACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCCCGCTTTATCg                                           >  1:45123/1‑251 (MQ=255)
                                                                                                                                                                                                       cAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGATCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACg                                   >  1:201727/1‑250 (MQ=255)
                                                                                                                                                                                                           atatTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCg                                           <  2:192711/238‑1 (MQ=255)
                                                                                                                                                                                                           atatTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCg                                           >  1:192711/1‑238 (MQ=255)
                                                                                                                                                                                                              ttGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGCTGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATAATGATTTAGTCCCGCTTTATCGTTTTCACGCAGctctc                           >  2:288338/1‑251 (MQ=255)
                                                                                                                                                                                                                                   ggaaaagtaCCCTGGATTGTCCCCTATTTTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGACGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTTTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCgg      <  2:295234/243‑1 (MQ=11)
                                                                                                                                                                                                                                      cacaaccccTGGAGTTGCTCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATGCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACg                                                   <  2:78373/197‑1 (MQ=2)
                                                                                                                                                                                                                                       aaGAACCCTTGATTTGCCCCTATATTTCCATACATCTGTGAACACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATATCCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGTTTCTTTGCTGCCGTTAACCCGTCTGGGTTGGGCATGATACTGATGTAGTCACGTTTTTTCGTGTTCACGAAGCTCTCTGCTATGCCGTTACTCCCCGGACTc  <  2:27417/251‑1 (MQ=11)
                                                                                                                                                                                                                                               |                                                                                                                                                                                                                                                  
GCTTGCAGATGGGTCGCAGGTTACTTCCTTGCGCACAGGATATAGTTATACCAGCGTTATTGTCGTTAGTGGTGAGTCGTCGGTATATCTGAATGGAGATACGACAATCAGCGGAGAATTCCCTCTGGGGTTTGCCGGGGTTATTCGGGTACAGGATAAAGCTTTGCTGGAAATTGGCAGTGGCGCTACGCTAACAATGCAGGATATTGACAGTTTTGAACATCATGGGACAAGAACCCTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTCCGGACTC  >  NC_000913/1467671‑1468152

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.