Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 1,223,636 IS2 (–) +5 bp intergenic (+187/+639) ymgJ → / ← minE uncharacterized protein YmgJ/Z‑ring positioning protein MinE

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1223636 =4 (0.160)24 (0.940) 20/486 0.3 85.5% intergenic (+187/+643) ymgJ/minE uncharacterized protein YmgJ/Z‑ring positioning protein MinE
?NC_000913 = 1469240 NA (NA)noncoding (1/1331 nt) IS2 repeat region
* ? NC_000913 = 12236404 (0.160)22 (0.860) 20/484 0.3 84.3% intergenic (+191/+639) ymgJ/minE uncharacterized protein YmgJ/Z‑ring positioning protein MinE
?NC_000913 1467910 = NA (NA)noncoding (1331/1331 nt) IS2 repeat region

AACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACT  >  NC_000913/1469045‑1469489
                                                                                                                                                                                                   |                                                                                                                                                                                                                                                         
aaCGAGGGAGACCGGCATCCCCGGTTCAAAGCTCTGCTTAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACt                                                                                                                                                                                                    <  1:251420/251‑1 (MQ=255)
 aCGAGGGAAGCCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGAACCTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACtt                                                                                                                                                                                                   <  1:5041/251‑1 (MQ=255)
   gAGGGAGACCGTCATCCCCGGTTCAAAGCCCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGt                                                                                                                                                                                                 <  1:65088/251‑1 (MQ=255)
     gggAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAg                                                                                                                                                                                               <  1:215194/251‑1 (MQ=255)
        agaCCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGAGAGGTACGCCGTCTGAGTTTCTCCGGCCCTAAGACATCAATCATCAGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTACGTGATATTGGTTGTATGGAGATTCAGGGGGCAAGTCTAAACCCCAGAACTTACTTATGCTGATTCGGGTGAGAAAATTGTTAATAAAGGTACTTGTAGAtt                                                                                                                                                                                            >  1:26749/1‑251 (MQ=17)
         ggccgccctCCCCGGTTCAAAGCACTGCTGATCAATTGCGAGTGTTGCATGTGTGGTATGACGACTGCCTTTCTCCGGCCCTAATCCATCAAGCATCTGGTCTCCAATGACTAGTCTAAAAACTAGGAGTAAGACTATCACTTATTTAAGTGAGATTGGTTGTCTGGAGATTCAGGGTGACAGTCTAAACTCTAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAATGTACTTGTATATTc                                                                                                                                                                                           <  2:79551/243‑1 (MQ=255)
         gaCCGGCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACTCCGGCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTc                                                                                                                                                                                           <  2:127863/251‑1 (MQ=255)
          aCCGTCATCCACGGTTCCAAGCTCTGCTGAAAACTTGCGATCTTTTCCTGTGCGGTACGCCGTCTGCGTTTCTCCGGCCCTCAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCATTATAAACACCAGAACTTAATTATGCTGATTCCGGTGCGAAATTTGTTAATAAAGGAACTTTTAGATTc                                                                                                                                                                                           >  2:170109/1‑250 (MQ=17)
             gTCATCACCGGTTCACAGCTCTGCTGAACTATTTCGATCTTTTCCTGTGTTGTACGCCGTCTGCGTTTCTCCGGCCCTATGACATCAATCATCTGTTCTACAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCTAGAACTTTCTTATGCTGATTCCGGTGCTAAAATTGTTAATAAAGGTACTTGTAGATTCAAtt                                                                                                                                                                                       <  2:66390/251‑1 (MQ=17)
                                                gATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTg                                                                                                                                                    >  2:63714/1‑251 (MQ=255)
                                                    ttttCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTAtgt                                                                                                                                                                   <  2:80955/232‑1 (MQ=255)
                                                    ttttCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTAtgt                                                                                                                                                                   >  1:80955/1‑232 (MQ=255)
                                                       tCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTACTAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGACAACATGGGGTTGCGGAGGt                                                                                                                                             >  1:134748/1‑251 (MQ=255)
                                                                    gtcggttGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACGAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATgaga                                                                                                                                <  2:263305/245‑1 (MQ=255)
                                                                                                                       cTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGCGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAAc                                                                             >  1:141980/1‑251 (MQ=255)
                                                                                                                                  aCTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCtt                                                                  <  1:27733/251‑1 (MQ=255)
                                                                                                                                                  tCACTTATTTAAGAGCTATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCAAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGact                                                  >  1:230530/1‑249 (MQ=255)
                                                                                                                                                          ttAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTgg                                           <  1:170109/250‑1 (MQ=255)
                                                                                                                                                                        ttGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGACAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGa                             >  2:290716/1‑250 (MQ=255)
                                                                                                                                                                                    tCAGGGGGCCAGTCTTAACCCCGGAACTTCCTGATGCTTATGCCGGTTCGAAAAATGTTAATAAAGGAACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGATACGAACATTTACAGCAGAGGAAAAAGCCACTGGTTTTGAACTATGGAAGAACGGAACAGTCTTCAGTGAAATAGCGAATATCCAGTGTCCAAAACCCGGAACGATCTTCACt                 <  2:239043/250‑1 (MQ=14)
                                                                                                                                                                                                  tAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACt  >  1:18629/1‑251 (MQ=255)
                                                                                                                                                                                                   aaaCCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATAc   <  1:63714/249‑1 (MQ=255)
                                                                                                                                                                                                   |                                                                                                                                                                                                                                                         
AACGAGGGAGACCGTCATCCCCGGTTCAAAGCTCTGCTGAACAATTGCGATCTTTTCCTGTGTGGTACGCCGTCTGCGTTTCTCCGGCCCTAAGACATCAATCATCTGTTCTCCAATGACTAGTCTAAAAACTAGTATTAAGACTATCACTTATTTAAGTGATATTGGTTGTCTGGAGATTCAGGGGGCCAGTCTAAACCCCAGAACTTACTTATGCTGATTCCGGTGCGAAAATTGTTAATAAAGGTACTTGTAGATTCAATTGGTCAACGCAACAGTTATGTGAAAACATGGGGTTGCGGAGGTTTTTTGAATGAGACGAACATTTACAGCAGAGGAAAAAGCCTCTGTTTTTGAACTATGGAAGAACGGAACAGGCTTCAGTGAAATAGCGAATATCCTGGGTTCAAAACCCGGAACGATCTTCACTATGTTAAGGGATACT  >  NC_000913/1469045‑1469489

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.