Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,223,636	IS2 (–) +5 bp	intergenic (+187/+639)	ymgJ → / ← minE	uncharacterized protein YmgJ/Z‑ring positioning protein MinE

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1223636 =	4 (0.160)	24 (0.940)	20/486	0.3	85.5%	intergenic (+187/+643)	ymgJ/minE	uncharacterized protein YmgJ/Z‑ring positioning protein MinE
	?	NC_000913	= 1469240	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 1223640	4 (0.160)	22 (0.860)	20/484	0.3	84.3%	intergenic (+191/+639)	ymgJ/minE	uncharacterized protein YmgJ/Z‑ring positioning protein MinE
	?	NC_000913	1467910 =	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

CGCGTTCCACGGGGATTCCACACCGGCACCATGGCTATACCCCACCCCGAGATGTCCGCTAAACGTACTGCTGAATGAGGCTTTAATAGCTGCCTGGTATATTCCACGTCTGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1223880‑1223636 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCT  <  NC_000913/1469240‑1469005                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   CGCGTCCCCCGGGGATGCCACACCGTCACCATGGCGATACCCCACCCCTAGATGTCCGCGAAACGTACTGCTGAATGAGGCTTTAATAGCTGCCTGGTATAGTCCACGTCGGCCCGACAAATCATTGACTAAATTACCGTCATTATTCACCTTCACCCGGTTATCAGCGACAAATTCTTTGCGCACAGCCGCCGTCAGCCAGGGGTCATTGCCATACCGATCCCCAGGCTCATGTTGTAACTCAGTAGACT                                                                                                                                                                                                                                        <  2:153161/251‑1                    tccgagctACCATGGCTATTCCCCACCCCGAGATGTCCGCTAAACTGACTGCTGAATGAGGCTTTAATAGCTGCCTTGTATATTCCTCGTCTGCCCGACAAAGCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCCGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAG                                                                                                                                                                                                                      <  2:248910/242‑1                            aaCATGGCTATACCCCACCCCGAGATGTCCGCTAAACGTACTGCTGAATGAGGCTTTAATAGCTGCCTGGTATATTCCACGTCTGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTAATTTCAATACCGATCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAG                                                                                                                                                                                                                      >  1:248910/3‑242                            ACCATGGCTATACCCCACCCCGAGATGTCCGCTAAACGTACTGCTGAATGAGGCTTTAATAGCTGCCTGGTATATTCCACGTCTGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCCGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAG                                                                                                                                                                                                                      >  1:248920/1‑242                            ACCATGGCTATACCCCACACCGAGATGGCCGCTAAACGGACTGCTGAATGAGGCTTTAATAGCTGCCTGGTATATTCCACGCCTGCCCGACAAATCATTGACGAAATTACCGTCATTATTCCCCTCCACCCGGTTATCATCGACAAAGTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCTAGGCGAATGTGGTAACTCAGTAGACTGGCACCCGGAATTTCCAG                                                                                                                                                                                                                      <  2:248920/242‑1                                  GCTATGCCCCACCCCGAGATGTCCGCTAAACGTACTGCTGAATGAGGCTTTAATAGCGTCCTGGTATATTCCACGTCGGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTGCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCTCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCATTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACT                                                                                                                                                                                                       <  2:104768/251‑1                                                            TAAACGTACTGATGAATGAGGCTTTAATAGCTGCCTGGTATATTCCACGTATGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCAGCCTTAACCCAGGGTTAATTTCCCTACCGTTCCCAAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACATACAATATCACTTTAATCAGTGATAGTCTTAATcata                                                                                                                                                                              >  2:156874/1‑246                                                             AAACGTACTGCTGAATGAGGCTTTAATAGCTGCCTGGTATATTCCACGTCTGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTATCATCGTCAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAG                                                                                                                                                                             <  1:54783/250‑1                                                              AACGTACTGCTGAATGAGGCTTTAATAGCTGCCTGGTATATTCCACGTCTGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTT                                                                                                                                                                           <  1:23992/251‑1                                                                     tgaTGAATGAGGCTTTAATAGCTGCCTGGTATATTCCACGTCTGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCCTGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAaac                                                                                                                                                                    >  2:294951/4‑248                                                                                        agtTGCCTGTTATATTCCACGTCGGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTAACATCGACAAATTTGTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTATTTTTTAGACTAGTCAGTGGAGAACAGA                                                                                                                                                  <  2:117017/247‑1                                                                                                GGTATATTCCACGTCTGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATG                                                                                                                                         >  1:4661/1‑251                                                                                                                   CGACAAATCATTGACGCAATTACCGTCTTTATTCACCTTCAACCGGTTATAATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGATTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAAACTGGCCCCCTGAATCTCCAGCCAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGTGCCGGAGAAACG                                                                                                                      >  2:158092/1‑251                                                                                                                             TTGACGAAATTACAGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCAC                                                                                                                                                                                                        >  1:62611/1‑159                                                                                                                                     aaTACCGTCATTATTCACCTTCACCCGGTTATCATCGACAAAGTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCGCCAGACAACCAATATCACTTAAATAAGTTATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGTGCCGGAGAAACGCAGACGGCGTTCCACACA                                                                                                    <  2:36928/249‑1                                                                                                                                         CCGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGA                                                                                                 >  1:114460/1‑250                                                                                                                                                                GTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAG                                                                         <  2:114460/251‑1                                                                                                                                                                                                  TTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCC                                       >  1:14147/1‑251                                                                                                                                                                                                  TTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAA                                                                                                                                                                                                   <  2:56670/95‑1                                                                                                                                                                                                  TTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAA                                                                                                                                                                                                   >  1:56670/1‑95                                                                                                                                                                                                                   TCCATACCGTTCCCCAGGCGCATGTTGTAACTCAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAG                       <  2:4661/250‑1                                                                                                                                                                                                                                       CATGTTGTAACACAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCATCATGGTGTAGCAGCCAGCCAGTTATTTCTCT  <  1:158092/251‑1                                                                                                                                                                                                                                                   CAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGAT                                                                                                                                          <  2:111710/103‑1                                                                                                                                                                                                                                                   CAGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGAT                                                                                                                                          >  1:111710/1‑103                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                   CGCGTTCCACGGGGATTCCACACCGGCACCATGGCTATACCCCACCCCGAGATGTCCGCTAAACGTACTGCTGAATGAGGCTTTAATAGCTGCCTGGTATATTCCACGTCTGCCCGACAAATCATTGACGAAATTACCGTCATTATTCACCTTCACCCGGTTATCATCGACAAATTCTTTGCGCACAGCCGCCTTCAGCCAGGGTTCATTTCCATACCGTTCCCCAGGCGCATGTTGTAACTCAG‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/1223880‑1223636 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCT  <  NC_000913/1469240‑1469005

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.