Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,938,972	IS2 (–) +5 bp	coding (746‑750/993 nt)	rbsR →	DNA‑binding transcriptional dual regulator RbsR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3938972 =	1 (0.030)	26 (0.670)	25/476	0.5	96.2%	coding (746/993 nt)	rbsR	DNA‑binding transcriptional dual regulator RbsR
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 3938976	2 (0.050)	24 (0.620)	20/482	0.9	92.2%	coding (750/993 nt)	rbsR	DNA‑binding transcriptional dual regulator RbsR
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAA  >  NC_000913/4499271‑4499760                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          gTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACAGCAGTATCATGCCCAAACCCTACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTAGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGTGCAAAT‑CCACCTTGTGCTg                                                                                                                                                                                                                                                 <  2:417444/251‑1 (MQ=255)   ccGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGat                                                                                                                                                                                                                                               >  1:291894/1‑251 (MQ=34)                gAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGaga                                                                                                                                                                                                                                  <  2:291894/251‑3 (MQ=39)                 aaTAGCAGAGAGCTTCGGGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGatat                                                                                                                                                                                                                                 <  2:78878/251‑1 (MQ=255)                       agggagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGATCCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGat                                                                                                                                                                                                                                               <  2:336033/228‑1 (MQ=21)                       agggagCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGat                                                                                                                                                                                                                                               >  1:336033/4‑231 (MQ=21)                                cGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACt                                                                                                                                                                                                                  >  2:388319/1‑251 (MQ=255)                                    aaaaCGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACttttt                                                                                                                                                                                                              >  1:319433/1‑250 (MQ=255)                                             aaGCGAGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGAAAAGAACCTTGCAGAGGCGTTCGAGCAATATAACGAATGGCATCCGCATAGTGCGCTGGGATATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAATACCAC‑TTGTGCTGATATGAATACGGATATTTTATTAAGAATACTTTTCAGTACAAGc                                                                                                                                                                                                     >  1:220528/1‑251 (MQ=14)                                                 gTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACt                                                                                                                                                                                                 <  1:225525/251‑1 (MQ=255)                                                    aCTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCACAT‑CAACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGt                                                                                                                                                                                               >  1:81320/1‑250 (MQ=255)                                                       aCATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTGGCAGCGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTAt                                                                                                                                                                                             <  2:365486/249‑1 (MQ=255)                                                                  aTGCCCAACCCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTACGTGATCACAGATGTCTGGAAATATCGGGGCAAAT‑CCAACTTGTGCTGATATGAATACGGATATTTTATACAGAATACtt                                                                                                                                                                                                                 >  2:399862/1‑218 (MQ=255)                                                                  aTGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCTGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtt                                                                                                                                                                                                                 <  1:399862/218‑1 (MQ=255)                                                                            cAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCTGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTAc                                                                                                                                                                      <  2:157219/251‑1 (MQ=255)                                                                                     tAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtt                                                                                                                                                                                                                 >  1:255748/1‑199 (MQ=255)                                                                                     tAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCAGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACtt                                                                                                                                                                                                                 <  2:255748/199‑1 (MQ=255)                                                                                                         gCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAgg                                                                                                                                         <  1:21665/251‑1 (MQ=255)                                                                                                           agagGCGTTCGAGCATTAAAAGGAATGGCATCCGCATTGTGCGCTGGGTTATATCCCGCCACGGGAATATCTGCGGCAGCGGGATTGTAATGGGTTAAGTGATAACAGAGGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTGTCAGTACAAGCGACTGTATTACAGTCTGCCATATAGGATTACCTGTGATCAGGAAGAATAAGGGGCTGAGGtt                                                                                                                                       <  2:209413/251‑1 (MQ=255)                                                                                                            gagGCGTTCGAGCATGTTAAAGAATTGCATCCCCATAGTGCGCTGGTTTCGCGCTCGCCACGGGAATATCTTCGGCAGCGGGCTTGTAATGGGGTAAGGTATAACAGATGGCTGGAAATATAGGTGCAAAT‑TCACCTTGTGCTGATATGAATACGGATAGTTTATACATAATACTTTTCAGTACAAGCGAATGTATTACAGTCTGCTATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAgggtt                                                                                                                                      <  2:318657/251‑4 (MQ=255)                                                                                                             agGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGATGGGTTATCGCTCGCCACGGGACAATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGGGCAGATATGAATACGGATATTTTATACAGAATAATTTTCAGTACAAGCGACTGTATTACAGTCTGCAATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTc                                                                                                                                     >  1:141479/1‑251 (MQ=25)                                                                                                                cGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCaaa                                                                                                                                  <  1:388319/251‑1 (MQ=255)                                                                                                                    cGAGCATTATAACGAATGGCATCCTCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTc                                                                                                                                     <  1:16753/244‑1 (MQ=255)                                                                                                                    cGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGGGGCTGAGGTTTc                                                                                                                                     >  2:16753/1‑244 (MQ=255)                                                                                                                                                  tGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGACTAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCaaaa                                                                                                 >  1:221040/1‑250 (MQ=255)                                                                                                                                                        gggTTATCGCTCGCCACGGGAATATCTTCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTGGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTTCAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCAt                                                                                            <  2:141479/249‑1 (MQ=255)                                                                                                                                                            catcgctgtccACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGGTAAGTGATAACAGATGTCTGGAAATATAGGGGCCAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCTAAAACCATAACAgg                                                                                      <  2:130214/242‑1 (MQ=255)                                                                                                                                                                      caagggAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGGGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGAGCAGTAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCt                                                                            <  2:221040/248‑1 (MQ=255)                                                                                                                                                                            aaTATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGCATACGGATATTTTATCCAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCAATATATGATTACCTGTGATCTGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCt                                                                      >  1:374280/1‑251 (MQ=255)                                                                                                                                                                                            ggCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGt                                                                        >  1:197030/1‑233 (MQ=255)                                                                                                                                                                                            ggCTTGGAATGGGTGAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGt                                                                        <  2:197030/233‑1 (MQ=255)                                                                                                                                                                                                tGTAATGGGTTCAACGATAACAGATGTCTGGAACTATAGGGGCAAAT‑CCACCTTGTGATGATATGAATACGGATATTTTATACAGAATACATTTCAGTACAAGAGACTGTATTACAGTCTGACATATATGATTACCTGTGATCCGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCAGGTATATTCCTCTATCCCTCAAAAACAATAACAGGTTAGTTATCTTCGTCTGTCAGCGGCTTGAATgtggg                                                  >  2:374569/1‑247 (MQ=255)                                                                                                                                                                                                                 tAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACaa                                  >  2:365302/1‑250 (MQ=255)                                                                                                                                                                                                                  aaCAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATTCTTCGCAGTACAAGCGACTGTATTACAGTCTGCCAGATATGATTTCCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTTTCTTCCGGTATATTCCTCTCTCCCTCAAAAACCATAACATGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTCTCAGTACAAAt                                <  2:413161/251‑1 (MQ=255)                                                                                                                                                                                                                   aCAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATa                               <  1:374356/251‑1 (MQ=255)                                                                                                                                                                                                                    cAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGAATATTTTCCGTACAAATAc                              >  1:138140/1‑251 (MQ=255)                                                                                                                                                                                                                       aTGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCgg                           >  1:348082/1‑251 (MQ=255)                                                                                                                                                                                                                        tGTCTGGAAATATAGGGGAAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGt                          >  1:121980/1‑251 (MQ=255)                                                                                                                                                                                                                           cTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCAt                       >  2:299051/1‑251 (MQ=255)                                                                                                                                                                                                                               aaaTATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCAc                   >  2:83856/1‑251 (MQ=255)                                                                                                                                                                                                                                 atatAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACTGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTCAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTg                 <  2:19423/251‑1 (MQ=255)                                                                                                                                                                                                                                                  aCCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTAACTGTGATCAGGAAGAATAAGAGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCATCAAAAACCATAAAAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTAAGTACAAATACCGGTCATCCACTGAAAACTACCTGCGac  >  1:358631/1‑249 (MQ=255)                                                                                                                                                                                                                                                  |                                                                                                                                                                                                                                                          GTCCGGAGAGTAACGGAATAGCAGAGAGCTTCGTGAAAACGATAAAGCGTGACTACATCAGTATCATGCCCAAACCAGACGGGTTAACGGCAGCAAAGAACCTTGCAGAGGCGTTCGAGCATTATAACGAATGGCATCCGCATAGTGCGCTGGGTTATCGCTCGCCACGGGAATATCTGCGGCAGCGGGCTTGTAATGGGTTAAGTGATAACAGATGTCTGGAAATATAGGGGCAAAT‑CCACCTTGTGCTGATATGAATACGGATATTTTATACAGAATACTTTTCAGTACAAGCGACTGTATTACAGTCTGCCATATATGATTACCTGTGATCAGGAAGAATAAGTGGCTGAGGTTTCAAACTGTCTGCCGGTATATTCCTCTCTCCCTTAAAAACCATAACAGGTTAGTTATCTTCGTCTGTCAGCGGATTGAATGGCGGTATATTTTCAGTACAAATACCGGTCATCCACTGAAAAATACCTGCGAA  >  NC_000913/4499271‑4499760

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.