Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,938,972	IS2 (–) +5 bp	coding (746‑750/993 nt)	rbsR →	DNA‑binding transcriptional dual regulator RbsR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3938972 =	1 (0.030)	26 (0.670)	25/476	0.5	96.2%	coding (746/993 nt)	rbsR	DNA‑binding transcriptional dual regulator RbsR
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 3938976	2 (0.050)	24 (0.620)	20/482	0.9	92.2%	coding (750/993 nt)	rbsR	DNA‑binding transcriptional dual regulator RbsR
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

GCAACGCAATATCTGATCGATAAAGGTCATACCCGTATCGCCTGTATTACCGGCCCGCTGGATAAAACTCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3938728‑3938976 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTC  <  NC_000913/4499511‑4499276                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        GCAACGCAATATCTGATCGATAAAGGTCATACCCGTATCGCCTGTATTACCGGCCCGCTGGATAAAACTCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTG                                                                                                                                                                                                                                            <  1:290803/251‑1     CGCAATATCTGATCGATAAAGGTCATACCCTTATCGCCTGTATTTCCGGCCCGCTGGATAAAACTCCGGCGCGCCTGCGGTTTGAAGGTTATCGGGCGGTGATGAAACGTGCGGGTCTCAACATTCCGGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTTCCGGAAATGACGCTATGGCTTGGATT                                                                                                                                                                                                                                        <  1:310353/251‑1     CGCAATATCTGATCGATAAAGGTCATACCCGTATCGCCTGTATTACCGGCCCGCTGGATAAAACTCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATT                                                                                                                                                                                                                                        <  1:38294/251‑1               GATCGATAAAGGTCATACCCGTATCGCCTGTATTACCGGCCCGCTGGATAAAACTCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTTATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTT                                                                                                                                                                                                                                             <  1:31282/236‑1                  CGATAAAGGTCAGTCCGGTCTCGCTTTTTTTACCTGCCCGTGGGATAAAACTCCGGGGCGTCTGCGGTTGGAAGTTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTGGAATATAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTTCCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATc                                                                                                                                                                                                                            <  2:241693/250‑2                                       CGCCTGTATTACCGGCCCGCTGGATAAAACTCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTA                                                                                                                                                                                                      <  1:15460/251‑1                                            GTATTACCGGCCCGCTGGATAAAACTCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCA                                                                                                                                                                                                 <  1:128355/251‑1                                              ATTATCGGTCCGCTGGATAAAACTCCGGCGCGACTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGAGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTATCCCAT                                                                                                                                                                                                <  2:178446/250‑1                                                                     cCCGGTGCGGCTGAGGTTGGAAGGTTATCGGGCGGTGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCATATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACAGCTGTTATCACTTAACCCATGACAAGCCCGATGCCGCAGATATT                                                                                                                                                                        <  2:403906/250‑1                                                                        GGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCACAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGAGTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCC                                                                                                                                                                     <  2:155187/251‑1                                                                                 GCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGC                                                                                                                                                            <  2:237466/251‑1                                                                                                                   GCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGT                                                                                                                           <  1:300535/250‑1                                                                                                                                                                           CGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTCTAATGCCCGAACGCCCCTGCAAGGTTCTTTTCTGCCGTTAACCCGTTTGGTTTGG                                                                  >  2:28020/1‑251                                                                                                                                                                                     GCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACT                                                        >  1:345964/1‑251                                                                                                                                                                                      agtTGCGTCAACTTCTATCACATCCGCTTTGTCCTGAGGACTGT‑GTTCCCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACAGCTGTTATCACTTAACCCATTCCAAGCCCTCTTCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATTCCATTCGTTATAATGCTCGAGCGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGAgacgg                                                       <  2:252337/248‑6                                                                                                                                                                                           CGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCACGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTTGGCCTGATACTGTTGT                                                   >  2:66988/1‑250                                                                                                                                                                                           CGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGTGCATGATACTGATGT                                                   >  1:50442/1‑250                                                                                                                                                                                              CAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTC                                               >  1:166268/1‑251                                                                                                                                                                                                                   CGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGGCATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGA                           >  1:127257/1‑250                                                                                                                                                                                                                    GTCCTCAGC‑CCGTCTTTACCGGAAATGACGCTATGGCTGGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGGTTTCACGAAG                         <  2:68171/251‑1                                                                                                                                                                                                                                GTCTTTACCGGAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTAT              >  1:271542/1‑251                                                                                                                                                                                                                                          GAAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTC    <  1:162874/251‑1                                                                                                                                                                                                                                           AAATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACACAGCGCACTATGCGGATGCCATTCGTTATAATGATCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATCCTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCc   >  2:146495/1‑250                                                                                                                                                                                                                                            AATGACGCTATGGCTTGGATTTGCCCCTATATTTCCAGACATATGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGACGCTCTCTGCTATTCCGTTACTCTC  >  2:107015/1‑251                                                                                                                                                                                                                                                                                                                                                                                                                                                                                                        GCAACGCAATATCTGATCGATAAAGGTCATACCCGTATCGCCTGTATTACCGGCCCGCTGGATAAAACTCCGGCGCGCCTGCGGTTGGAAGGTTATCGGGCGGCGATGAAACGTGCGGGTCTCAACATTCCTGATGGCTATGAAGTCACTGGTGATTTTGAATTTAACGGCGGGTTTGACGCTATGCGCCAACTGCTATCACATCCGCTGCGTCCTCAGG‑CCGTCTTTACCGGAAATGACGCTATGGCT‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/3938728‑3938976 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TGGATTTGCCCCTATATTTCCAGACATCTGTTATCACTTAACCCATTACAAGCCCGCTGCCGCAGATATTCCCGTGGCGAGCGATAACCCAGCGCACTATGCGGATGCCATTCGTTATAATGCTCGAACGCCTCTGCAAGGTTCTTTGCTGCCGTTAACCCGTCTGGTTTGGGCATGATACTGATGTAGTCACGCTTTATCGTTTTCACGAAGCTCTCTGCTATTCCGTTACTCTC  <  NC_000913/4499511‑4499276

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.