Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,938,972	IS2 (–) +5 bp	coding (746‑750/993 nt)	rbsR →	DNA‑binding transcriptional dual regulator RbsR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3938972 =	1 (0.030)	26 (0.670)	25/476	0.5	96.2%	coding (746/993 nt)	rbsR	DNA‑binding transcriptional dual regulator RbsR
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 3938976	2 (0.050)	24 (0.620)	20/482	0.9	92.2%	coding (750/993 nt)	rbsR	DNA‑binding transcriptional dual regulator RbsR
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

AATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATAC  >  NC_000913/4497963‑4498430                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                           acttattCTAGGAATATTTACTTTGTTAAACTAGACAAACGGTATTAACTGATGTATTAACTTTTTAAAGCTCACTGCAGGGGTGATTATCAGAATCCGAGTTTATTCATTATTTAAATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGAGATGCGGGATAACTAACGTAAGGAGTGCTGAAGGAATTTGATGGTCACTTGACGACTGGACCCAGGAATCTACAGCCAACAAat                                                                                                                                                                                                                            >  2:268909/3‑251 (MQ=255)      ttCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTTCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGa                                                                                                                                                                                                                                             <  2:364070/229‑1 (MQ=255)      ttCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTTCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGa                                                                                                                                                                                                                                             >  1:364070/1‑229 (MQ=255)          aGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTaa                                                                                                                                                                                                                   <  1:282517/251‑1 (MQ=255)                   ttCTTTGTTAAAAAAGACAAACGGTATTAACTGATGGATTTACTATTTACCGCTCCCTGCAGGGGGGATGATCAGAATCGGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACGGACGTAAGGAGTGCTTCAGGGATTTGATGGTCCCTTGTAGACTGGCCCTCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGa                                                                                                                                                                                                           <  1:269521/250‑1 (MQ=255)                       ttGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTc                                                                                                                                                                                                      >  1:349128/1‑251 (MQ=255)                         gTTAAAAAAGACAAACGGTATTAACGAATGTATTTACTATTTACCGCTCCCTGCAGTGTGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATGCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCtt                                                                                                                                                                                                    <  2:404754/251‑1 (MQ=255)                                   aCAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGGCTTAATACTAGtt                                                                                                                                                                                          <  1:352164/251‑1 (MQ=255)                                   aCAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGAGATAGTCTTAATACTAGtt                                                                                                                                                                                          >  1:364736/1‑251 (MQ=255)                                                   gATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAt                                                                                                                                                                            <  1:150359/249‑1 (MQ=255)                                                    aTGTATTTACTATTTACCGCTCCCTGCAGGGGGGCTTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGGGATAGTCTTAATACTAGTTTTTAGACTAGTCATTgg                                                                                                                                                                         >  2:270376/1‑251 (MQ=255)                                                       tatttactatttacCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTGTTTAGACTAGTCATTGg‑ag                                                                                                                                                                      <  2:347232/250‑1 (MQ=255)                                                        atttactatttacCTCTCCCTGCAGGGGGGATTAACAGAATCCTCGGTTATTCATTATTTATATACATGCAAGAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGTAGAACTGACGCAAGGAGTGCTGCAGGGATTGGATTGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTGATACTAGTTTTTAGACTAGTCATTGg‑aga                                                                                                                                                                     <  2:233048/250‑1 (MQ=37)                                                                      gCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGt                                                                                                                                                      <  1:272007/251‑1 (MQ=255)                                                                           cTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCtt                                                                                                                                                   >  2:312697/1‑249 (MQ=255)                                                                               aGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGcc                                                                                                                                             >  1:378747/1‑251 (MQ=255)                                                                                ggggggATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCAACTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTGTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCg                                                                                                                                            <  2:22385/251‑1 (MQ=255)                                                                                 gggggATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCgg                                                                                                                                           >  1:195123/1‑251 (MQ=255)                                                                                                            tattattTATATACATTCAATAAACCAGTAACCTCCTAAAAAATCCTGTACTCACCGTGTGATACGGGATATCTGACGTTACGAGTGCTGCAGGGCTTTGTTGTTACCTTGTAGACTGGCCCCCTGAATCTCCAGACCACCAATATCACTTAAATACGTGATAGTCTTAATACTAGTTTTTATCCTGTTCATTGGTAAAAC‑GATGATTGATGTATTATGGCCGGTGACACGCAgccgtagtacaacaca                                                                                                                  >  2:417458/2‑234 (MQ=255)                                                                                                                    atataCATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGaaa                                                                                                             >  1:254593/1‑246 (MQ=255)                                                                                                                           ttCAATAACAAAGAAACCTCATAAAAAATCCTGTACTCACAGGGTGATGAGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATAGTCCCTTGAAGACTGGCCCCCTGAATCTCCCGACAACCAATATCACTTAACTAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGACAAGCAGACGACGTACCAAACTGGAACAGATCGCAATTg                                                                                                 >  2:298204/1‑251 (MQ=18)                                                                                                                                 aacaaaGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCCCACAGGTAAAGATCGCAATTGTTcagc                                                                                           >  2:40760/4‑251 (MQ=255)                                                                                                                                     aaGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCagag                                                                                       <  1:406469/251‑1 (MQ=255)                                                                                                                                      aGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGc                                                                                      <  1:26834/251‑1 (MQ=255)                                                                                                                                              cATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGaa                                                                                <  2:349128/249‑1 (MQ=255)                                                                                                                                                tAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCgg                                                                            >  1:357800/1‑251 (MQ=255)                                                                                                                                                                    gggTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGtt                                                        <  1:40760/251‑1 (MQ=255)                                                                                                                                                                                   aCTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGgt                                         >  1:322429/1‑251 (MQ=255)                                                                                                                                                                                                  accacaattgcccggtgaaatggggTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTCCCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTGGTTGCCCGGCAACATGGTGTAGCagccagcc                            >  2:154119/25‑249 (MQ=11)                                                                                                                                                                                                    ggcAGGGATTTGATGGACCATTGTAGACTGGCCCCCTGAATCTCCAGACAAACAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGTCAGtt                        <  2:105331/250‑1 (MQ=255)                                                                                                                                                                                                           aTTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGAGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTtctc                 >  1:149906/1‑251 (MQ=37)                                                                                                                                                                                                                          gTAGACTGGCCCCCTTAATCTCCAGACAACCAATATCACGTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACTGCGTACCACACAGGAAAAGATCGCAATTGTGCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCTTCGTTGCCCGGCAACATGGTGTATCGGCCAGCCAGTTATTTCTCTGGCGTAAGCAATAc  <  2:20485/251‑1 (MQ=11)                                                                                                                                                                                                                           |                                                                                                                                                                                                                                                           AATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGG‑AGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATAC  >  NC_000913/4497963‑4498430

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.