Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,938,983	IS2 (–) +5 bp	coding (757‑761/993 nt)	rbsR →	DNA‑binding transcriptional dual regulator RbsR

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	3938983 =	0 (0.000)	33 (0.760)	27/486	0.6	100%	coding (757/993 nt)	rbsR	DNA‑binding transcriptional dual regulator RbsR
	?	NC_000913	4498181 =	NA (NA)					noncoding (1/1331 nt)	IS2	repeat region
*	?	NC_000913	= 3938987	0 (0.000)	41 (0.950)	33/486	0.3	100%	coding (761/993 nt)	rbsR	DNA‑binding transcriptional dual regulator RbsR
	?	NC_000913	= 4499511	NA (NA)					noncoding (1331/1331 nt)	IS2	repeat region

AACAGTAATCATCCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATA  >  NC_000913/4497933‑4498429                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         aaCAGTAATCATCCCGGGCAACTGCCCGGAAATTATTCTAGGATTATTTTCTTTGTTAAACCAGACAAACGGAATTAACTGATGTATTTACTATTTACCGCTCACTGCAGGGGAGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAACGTAACCTCATAAAAAATACTGTACTCACAGGGTGATGCGGGATAACTAACGAAAGGAGTGATGCAGGGATTTGGTGGTCCCTTGt                                                                                                                                                                                                                                                          >  2:19558/1‑249 (MQ=255)                      aTGCCCGGGAATTATTCTATGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAg                                                                                                                                                                                                                                   <  1:35006/251‑1 (MQ=255)                      aTGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACCTTCAATAAAAAAGTAACCTCATAAAAAATCCTGAACTCACAGGGTGATGCGGGATAACTGACGTAAGAAGTGCTGCAGGGATTTGTTGGTCCCTTGTAAACTGGCCCACTGAAtctca                                                                                                                                                                                                                                     >  2:353051/1‑248 (MQ=255)                                  tattCTAGGATTATTTTCTTTGTTAACAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGGCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAatat                                                                                                                                                                                                                        <  2:326540/250‑1 (MQ=255)                                       tAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACtt                                                                                                                                                                                                                   >  1:378829/1‑250 (MQ=255)                                                       gTTAAAAAAGACAAACGGTATTAACTGGTGTATTTACTATTTACCGCTACATAAAGGGGAGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACATCATAAAAAATCCTGTACTAACAGGGTGATGCGGGATAACTGACGTAAGGCGTGCTGCAGGGATTTGATGGTCCCTTGAAGACTGGCCCCCTGAATCTCCAGACAACAAATATCACTTAAATAAGTGATAGTCtt                                                                                                                                                                                                  >  2:257475/1‑251 (MQ=255)                                                                gACAAACGGTATTAACTGGTGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGCATCTCCAGACCACCAATATc                                                                                                                                                                                                                       >  2:22714/1‑221 (MQ=255)                                                                gACAAACGGTATTAACTGATGTATTTTCTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGGTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGAGAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATc                                                                                                                                                                                                                       <  1:22714/221‑1 (MQ=255)                                                                gACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGTATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTTCTGCAGGGATTTGATGGTCCCTTGTAGACTTGCCCCCTGAATCTCCAGACATCCAATATCACTTAAATAAGTGATAGTCTTAATACTAGt                                                                                                                                                                                         <  1:254201/251‑1 (MQ=255)                                                                             aaCTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGt                                                                                                                                                                             <  1:247391/250‑1 (MQ=255)                                                                               cTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCAt                                                                                                                                                                          >  1:373040/1‑251 (MQ=255)                                                                                     tctttactatttacCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCACTAAAAAAGCAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTgg                                                                                                                                                                       >  2:230129/3‑248 (MQ=255)                                                                                                tacCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTACCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGAtgattg                                                                                                                                                         >  1:247300/1‑251 (MQ=255)                                                                                                           gCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAggg                                                                                                                                              <  1:176585/251‑1 (MQ=255)                                                                                                                      tATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACg                                                                                                                                   >  1:451130/1‑251 (MQ=255)                                                                                                                                       aTTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCC‑TTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACcacac                                                                                                                  >  1:332046/1‑250 (MQ=255)                                                                                                                                               tttatATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCAATGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGAAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAATCTTAATACTAGTTTTTAGACTAGTCCTTGGCGAACAGATGATTGATGTCTTAGGGCCGGAGAGACGCAGACGGCGAACCACACAGGaaatg                                                                                                          >  2:216580/1‑249 (MQ=21)                                                                                                                                                                     gTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCt                                                                                    >  1:326224/1‑251 (MQ=255)                                                                                                                                                                       aaCCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCttt                                                                                  >  1:130545/1‑251 (MQ=255)                                                                                                                                                                            cATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGaa                                                                               <  1:251421/249‑1 (MQ=255)                                                                                                                                                                                       ctatACTCACCGGGAGATGCGGGATAACTGACGAAAGGAGAGCTGCAGAGATTTGACGGTCCCTTGTCGACTGGCCCCCTGAATGCCCAGACACCCAATATCACTTAAATAAGTGATAGTCTTAATACTCGTTTTTAGACTAGTCATTGGAGAAAAGATGATTGCTGTCTTAGGGCCGAAGAAACGCAGACAACGAACCACCCAGGAAAAGATCGAAATTATTAAGCAGTGCTTTTAACCGGGTATAACgg                                                                  >  2:449868/4‑251 (MQ=9)                                                                                                                                                                                             tCACAGGGTGATGCGGGATAACTGACGTACGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGACTCTCCCGACAACCAATATCACTTAAATACGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGCGCTTTGAACCGGGGATGAGGGTCTccc                                                            >  2:83881/1‑251 (MQ=255)                                                                                                                                                                                               acaGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTc                                                          >  1:366960/1‑251 (MQ=255)                                                                                                                                                                                                           gggATAACTAACGTAAGGAGTGATGCAGGGATTTGATGGTCACTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGAGATAGTCTTAATACTAGTTTTTAGCCTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGCGAAACGCAGCCGGCGTACCACACGGGAAAAGATCGCACTTGTTCAGCAGAGCTTTGAACCGGGGATACCGGTCTCCCTCGTTGCCCGGCa                                               >  2:195473/1‑250 (MQ=14)                                                                                                                                                                                                                                       ggATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTtc                  >  1:411025/1‑251 (MQ=38)                                                                                                                                                                                                                                            tGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACGAGTCATTGGAGAACAGATGATTGATGTCGTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGGTATTTCTCTg              <  2:212457/250‑1 (MQ=255)                                                                                                                                                                                                                                              aTGGTCCCGTGTAGACTGGCCCCCTTAATCTCCATACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCCTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAATATCGCAATTGTTCAGCAGAGCTTTGAACCTGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCg           <  2:326224/251‑1 (MQ=255)                                                                                                                                                                                                                                                       tGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATATCTTAGAGCCGGAGAAACGCAGACGACGTACCACACAGGAAAAGATCGCAATTGATCAGCAGAGCTTTGAACCGGGGCTGACGGTCTACCACGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAAtc  >  1:72753/1‑250 (MQ=11)                                                                                                                                                                                                                                                       tGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGGTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAAt   <  2:460505/250‑1 (MQ=11)                                                                                                                                                                                                                                                         |                                                                                                                                                                                                                                                         AACAGTAATCATCCCGGGCAAATGCCCGGGAATTATTCTAGGATTATTTTCTTTGTTAAAAAAGACAAACGGTATTAACTGATGTATTTACTATTTACCGCTCCCTGCAGGGGGGATTATCAGAATCCGCGGTTATTCATTATTTATATACATTCAATAAAAAAGTAACCTCATAAAAAATCCTGTACTCACAGGGTGATGCGGGATAACTGACGTAAGGAGTGCTGCAGGGATTTGATGGTCCCTTGTAGACTGGCCCCCTGAATCTCCAGACAACCAATATCACTTAAATAAGTGATAGTCTTAATACTAGTTTTTAGACTAGTCATTGGAGAACAGATGATTGATGTCTTAGGGCCGGAGAAACGCAGACGGCGTACCACACAGGAAAAGATCGCAATTGTTCAGCAGAGCTTTGAACCGGGGATGACGGTCTCCCTCGTTGCCCGGCAACATGGTGTAGCAGCCAGCCAGTTATTTCTCTGGCGTAAGCAATA  >  NC_000913/4497933‑4498429

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.