Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,521,130	Δ122 bp		valY	valY

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2521130	2521251	122	25 [0]	[5] 30	valY	valY

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2521129	0 (0.000)	25 (0.780)	21/326	0.5	100%	intergenic (+3/‑44)	valX/valY	tRNA‑Val/tRNA‑Val
	?	NC_000913	2521252 =	0 (0.000)					intergenic (+4/‑1)	valY/lysV	tRNA‑Val/tRNA‑Lys

ACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCG  >  NC_000913/2521007‑2521497                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      gttgactcattttgaactctccctataatgcgactccacacagcgggggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccaactactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTc                                                                                                                                                                                                                                                  <  1:16641‑M2/6‑1 (MQ=255) gttgactcattttgaaccctccctataatgctactccccacagcgggggtgattagcacagctggtagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccaactactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaatgagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTc                                                                                                                                                                                                                                                  <  1:349224‑M2/6‑1 (MQ=255)                      cctataatgcgactccacacagcgggggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccaactactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTTGCTCAGTTGGTAGAGc                                                                                                                                                                                                                              >  1:121562‑M2/225‑250 (MQ=255)                                            cgtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccaactactttatgtattcgccgctgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggatggggtcggcggttcgagcccgtcatcacccatcacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGt                                                                                                                                                                                                        <  1:44724‑M2/48‑1 (MQ=255)                                                   gtttacctcatctgggtgggcacctccctgacaagggggggggcggcggttcgattccgtcagcacccgccaactactttatgtagtctccgccgtgtagcaagacattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatctcccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                                                                                                                                <  2:359109‑M2/56‑1 (MQ=255)                                                   gattagctcagctgggagagcaccttccttacaaggagggggtcggcggttcgatcccgtcatcacccaccaactactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGtt                                                                                                                                                                                                <  1:289082‑M2/56‑1 (MQ=255)                                                                aggagagcacctaccttacaaggagggggtcggcggttcgatcccgtcatcacccaccaactactttaagtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagcttggagagcacctcccttacaaggagggggtcgtcggttcgatcctgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAAGCAATTGGTCGCAGGTTCGAATCCTGCAc                                                                                                                                                                                    <  1:205826‑M2/68‑1 (MQ=255)                                                                                tacaaggagggggtcggcggttcgatcccgtcatcacccaccaactactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccactgCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATGGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTaa                                                                                                                                                                     <  2:315764‑M2/83‑1 (MQ=255)                                                                                    aagagggggtcggctgttcgatcccgtcatcacccaccaactactgtatgtagtctgcgccgtgtagcaagaaattgagacgtgggtgattagctcagctgggagagcacctcccttacaaggaggggttcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTTGTAGAGCAGTTGACTTTTAATCAGTGGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAgc                                                                                                                                                               <  1:319502‑M2/89‑1 (MQ=255)                                                                                              cggctggtcgatcccgtcatcacccaccaactactttatgtaatctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAgg                                                                                                                                                     <  2:398809‑M2/99‑1 (MQ=255)                                                                                                gcggttcgatcccgtcatcacccaccaactacgttatgtagtcgccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg                                                                                                                                                   <  2:141228‑M2/101‑1 (MQ=255)                                                                                                gcggttcgatcccgtcatcaaccaccaactactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGgcg                                                                                                                                                   <  2:95190‑M2/101‑1 (MQ=255)                                                                                                   gttcgatcccgtcaacaccccccaactactttatgtattctccgccgtgtagcaagaaagtgagaagtgggtgattagctcagttgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcccccaccccttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGCCTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtt                                                                                                                                                <  1:495589‑M2/104‑1 (MQ=255)                                                                                                      cgatcccgtcatcacccaccatatactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttt                                                                                                                                              <  1:281652‑M2/106‑1 (MQ=255)                                                                                                       gatcccgtcatcacccaccacctactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttagatcccgtcatcacccaccacttCGGGTCGTTAGCTCCGTTGGTAGAGCAGTTGAATTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCtttttt                                                                                                                                            >  1:429678‑M2/144‑251 (MQ=255)                                                                                                               catcacccaccaactactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCTCTTTTTTACTATCt                                                                                                                                     <  1:145974‑M2/115‑1 (MQ=255)                                                                                                               catcaaccaccaactactttatgtagtctccgccgtgtagcaagaaatggagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgagcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGTTCGCAGGTTCGAATCCTGCATGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCt                                                                                                                                     <  1:303317‑M2/115‑1 (MQ=255)                                                                                                                  cacccaccaactactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCg                                                                                                                                  <  1:465557‑M2/118‑1 (MQ=255)                                                                                                                           actactttatgtagtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAg                                                                                                                        <  1:249558‑M2/128‑1 (MQ=255)                                                                                                                                   atgtattccccgccgtgtagcaagaaattgaaaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAAc                                                                                                                <  2:119756‑M2/136‑1 (MQ=255)                                                                                                                                        gtctccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAGCCTgca                                                                                                           <  2:214295‑M2/141‑1 (MQ=255)                                                                                                                                           tccgccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGGCGATAGCACAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGACGCAAGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAAGAGCTTTTTTAATATCTGCGATACTCAAAAATTCGAACCTgcagc                                                                                                         >  2:306869‑M2/108‑250 (MQ=255)                                                                                                                                              gccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccccttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAAGTTCGAATCCTGCACGGCCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGt                                                                                                     >  1:51884‑M2/105‑251 (MQ=255)                                                                                                                                              gccgtgtagcaagaaattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGt                                                                                                     <  2:108848‑M2/147‑1 (MQ=255)                                                                                                                                                            aattgagaagtgggtgattagctcagctgggagagcacctcccttacaaggagggggtcggcggttcgatcccgtcatcacccaccacttCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCa                                                                                       <  1:368677‑M2/161‑1 (MQ=255)                                                                                                                                                                      tGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGAGGGTCGATACCGTCATCCCCAACAAATTGGGGTCGGTCTCTCAGTTGGTAGAGCAGTTGACATTTAATCAAATGGGCGCAGAGTCGAAACCTGCCAGAACCACCAATGTAAAAAAGCGCCCTAAAGTAGCTTTTTTACTATCTTCGATACTAAAAGATTTGAACCTGCAGCAGGTTTGACTTGCGCGCAACGAaacaa                                                                              >  2:503785/1‑250 (MQ=16)                                                                                                                                                                       gggTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGTGGTCGTCGGTTCGATCCCGGCATCTCCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACGCAAAGATTCGAACCTGCAGCAGGTTGGAGTTGATCGCAGCGAAACAACg                                                                            <  2:215812/251‑1 (MQ=18)                                                                                                                                                                                                                                       catcgtgccctacTTCGGATCGTTAGATCATTGGGTAGAGCAGTTGACTTTTAATCAAAAGGTCGCAGGATAGAATCCTGTAAGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGAGCCGAACCGGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACTGCCCGATGGGCGAGCGAAGCGAGTCATCCTGCACAACCCTCCAATg            <  2:121562/240‑1 (MQ=11)                                                                                                                                                                                                                                             ccaccaCTTCGGGGCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATACTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGAGAGTCATCCTGCACGACCCACCAATGTaaaa       >  1:327096/1‑250 (MQ=255)                                                                                                                                                                                                                                                 cacTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAgcg  <  1:306869/251‑1 (MQ=33)                                                                                                                                                                                                                                                      |                                                                                                                                                                                                                                                      ACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAATCAATTGGTCGCAGGTTCGAATCCTGCACGACCCACCAATGTAAAAAAGCGCCCTAAAGGCGCTTTTTTACTATCTGCGATACTCAAAGATTCGAACCTGCAGCAGGTTTGAGTTGAGCGCAGCGAAACAACGGAGCCGCTCGCGGCGACGGCCCGAAGGGCGAGCGAAGCGAGTCATCCTGCACGACCCACCAATGTAAAAAAGCG  >  NC_000913/2521007‑2521497

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑