Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,521,130	Δ122 bp		valY	valY

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2521130	2521251	122	25 [0]	[5] 30	valY	valY

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2521129	0 (0.000)	25 (0.780)	21/326	0.5	100%	intergenic (+3/‑44)	valX/valY	tRNA‑Val/tRNA‑Val
	?	NC_000913	2521252 =	0 (0.000)					intergenic (+4/‑1)	valY/lysV	tRNA‑Val/tRNA‑Lys

GTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAA  >  NC_000913/2520885‑2521290                                                                                                                                                                                                                                                     |                                                                                                                                                                  gTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtc                                                                                                                                                             <  1:16641‑M1/251‑7 (MQ=255) gTTGACTCATTTTGAACCCTCCCTATAATGCTACTCCCCACAGCGGGGGTGATTAGCACAGCTGGTAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAATGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtc                                                                                                                                                             <  1:349224‑M1/251‑7 (MQ=255)                      ccTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgtttgctcagttggtagagc                                                                                                                                         >  1:121562‑M1/1‑224 (MQ=255)                                            cgtgggtgaTTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTATTCGCCGCTGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGATGGGGTCGGCGGTTCGAGCCCGTCATCACCCATCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggt                                                                                                                   <  1:44724‑M1/247‑49 (MQ=255)                                                   gtttacctcatctGGGTGGGCACCTCCCTGACAAGGGGGGGGGCGGCGGTTCGATTCCGTCAGCACCCGCCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGACATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCTCCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggtt                                                                                                           <  2:359109‑M1/245‑57 (MQ=255)                                                   gATTAGCTCAGCTGGGAGAGCACCTTCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggtt                                                                                                           <  1:289082‑M1/251‑57 (MQ=255)                                                                aggaGAGCACCTACCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTAAGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTTGGAGAGCACCTCCCTTACAAGGAGGGGGTCGTCGGTTCGATCCTGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaagcaattggtcgcaggttcgaatcctgcac                                                                                               <  1:205826‑M1/249‑69 (MQ=255)                                                                                tACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTGcgggtcgttagctcagttggtagagcagttgacttttaatcaatgggtcgcaggttcgaatcctgcacgacccaccaatgtaa                                                                                <  2:315764‑M1/249‑84 (MQ=255)                                                                                    aagaggggGTCGGCTGTTCGATCCCGTCATCACCCACCAACTACTGTATGTAGTCTGCGCCGTGTAGCAAGAAATTGAGACGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGTTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagtttgtagagcagttgacttttaatcagtgggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagc                                                                          <  1:319502‑M1/249‑90 (MQ=255)                                                                                              cggctggtcgatcccGTCATCACCCACCAACTACTTTATGTAATCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaagg                                                                <  2:398809‑M1/244‑100 (MQ=255)                                                                                                gcggTTCGATCCCGTCATCACCCACCAACTACGTTATGTAGTCGCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcg                                                              <  2:141228‑M1/251‑102 (MQ=255)                                                                                                gcggTTCGATCCCGTCATCAACCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcg                                                              <  2:95190‑M1/251‑102 (MQ=255)                                                                                                   gTTCGATCCCGTCAACACCCCCCAACTACTTTATGTATTCTCCGCCGTGTAGCAAGAAAGTGAGAAGTGGGTGATTAGCTCAGTTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCCCCCACCCCTTcgggtcgttagctcagttggtagagcagttgccttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcgctt                                                           <  1:495589‑M1/251‑105 (MQ=255)                                                                                                      cGATCCCGTCATCACCCACCATATACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcgctttt                                                         <  1:281652‑M1/250‑107 (MQ=255)                                                                                                       gATCCCGTCATCACCCACCACCTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTAGATCCCGTCATCACCCACCACTTcgggtcgttagctccgttggtagagcagttgaattttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcgctttttt                                                       >  1:429678‑M1/1‑143 (MQ=255)                                                                                                               catcaCCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggctcttttttactatct                                                <  1:145974‑M1/250‑116 (MQ=255)                                                                                                               catcaACCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATGGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGAGCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattgttcgcaggttcgaatcctgcatgacccaccaatgtaaaaaagcgccctaaaggcgcttttttactatct                                                <  1:303317‑M1/250‑116 (MQ=255)                                                                                                                  cacccaccAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcgcttttttactatctgcg                                             <  1:465557‑M1/250‑119 (MQ=255)                                                                                                                           actactTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcgcttttttactatctgcgatactcaaag                                   <  1:249558‑M1/251‑129 (MQ=255)                                                                                                                                   aTGTATTCCCCGCCGTGTAGCAAGAAATTGAAAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcgcttttttactatctgcgatactcaaagattcgaac                           <  2:119756‑M1/251‑137 (MQ=255)                                                                                                                                        gTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcgcttttttactatctgcgatactcaaagattcgagcctgca                      <  2:214295‑M1/251‑142 (MQ=255)                                                                                                                                           tcCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcggggcgatagcacagttggtagagcagttgacttttaatcaattggacgcaagttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaagagcttttttaatatctgcgatactcaaaaattcgaacctgcagc                    >  2:306869‑M1/1‑107 (MQ=255)                                                                                                                                              gccgTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCCCTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaagttcgaatcctgcacggcccaccaatgtaaaaaagcgccctaaaggcgcttttttactatctgcgatactcaaagattcgaacctgcagcaggt                >  1:51884‑M1/1‑104 (MQ=255)                                                                                                                                              gccgTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcgcttttttactatctgcgatactcaaagattcgaacctgcagcaggt                <  2:108848‑M1/251‑148 (MQ=255)                                                                                                                                                            aaTTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTcgggtcgttagctcagttggtagagcagttgacttttaatcaattggtcgcaggttcgaatcctgcacgacccaccaatgtaaaaaagcgccctaaaggcgcttttttactatctgcgatactcaaagattcgaacctgcagcaggtttgagttgagcgca  <  1:368677‑M1/251‑162 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                  GTTGACTCATTTTGAACTCTCCCTATAATGCGACTCCACACAGCGGGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCAACTACTTTATGTAGTCTCCGCCGTGTAGCAAGAAATTGAGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTTCTCGCCAGCTAAATTTCTTGTAAAAATGTGAAGTACCGAAGTGGGTGATTAGCTCAGCTGGGAGAGCACCTCCCTTACAAGGAGGGGGTCGGCGGTTCGATCCCGTCATCACCCACCACTTCGGGTCGTTAGCTCAGTTGGTAGAGCAGTTGACTTTTAA  >  NC_000913/2520885‑2521290

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑