Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,291,049	IS1 (+) +9 bp	coding (808‑816/1014 nt)	rssB →	regulator of RpoS

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	257908 =	NA (NA)	31 (0.550)	29/492	0.8	93.7%	noncoding (768/768 nt)	IS1	repeat region
	?	NC_000913	1291049 =	2 (0.040)					coding (808/1014 nt)	rssB	regulator of RpoS
*	?	NC_000913	= 258675	NA (NA)	41 (0.730)	38/492	0.5	95.2%	noncoding (1/768 nt)	IS1	repeat region
	?	NC_000913	= 1291057	2 (0.040)					coding (816/1014 nt)	rssB	regulator of RpoS

ACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAG  >  NC_000913/258430‑258680                                                                                                                                                                                                                                                      |      acgacatgcATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGGTTCCATGTTTTACGGCAGTGAGAGCAGAGATAGCTCTGATGTCCGGCTGTGCTTTTGCCGTTACGCACCACCCCGGCAGTAGCTGAACAGGAGGGACATCTGATAGAAACAGAATCCATTGTAGCACCTCAAAAACACCATCATACACTAATTCATTAAGTTGGCCGCATTACCTgtaa  <  2:341565/243‑4 (MQ=11)                                                                                                                                                                                                                                                      |      ACGCCATTCATGGCCATATCAATGATTTTCTGGTGCGTACCGGGTTGAGAAGCGGTGTAAGTGAACTGCAGTTGCCATGTTTTACGGCAGTGAGAGCAGAGATAGCGCTGATGTCCGGCGGTGCTTTTGCCGTTACGCACCACCCCGTCAGTAGCTGAACAGGAGGGACAGCTGATAGAAACAGAAGCCACTGGAGCACCTCAAAAACACCATCATACACTAAATCAGTAAGTTGGCAGCATCACCTGAAG  >  NC_000913/258430‑258680

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 37 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.