Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
MC JC	NC_000913	2,942,775	(GTTGTGTT)2→1	noncoding (80‑87/206 nt)	gcvB →	small regulatory RNA GcvB

Missing coverage evidence...
			seq id	start	end	size	←reads	reads→	gene	description
*	*	÷	NC_000913	2942775	2942782	8	30 [1]	[0] 29	gcvB	small regulatory RNA GcvB

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	= 2942774	1 (0.030)	29 (0.880)	27/468	0.3	98.4%	noncoding (79/206 nt)	gcvB	small regulatory RNA GcvB
	?	NC_000913	2942783 =	0 (0.000)					noncoding (88/206 nt)	gcvB	small regulatory RNA GcvB

GTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTATCGTTATCAGTCTG  >  NC_000913/2942534‑2943022                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                aaaaactcgtaaggcatttagcggtggtaatcgtttagacatggctattaaactttgatgttaaatgaatttaacaattatatcacactatgtaacctattaggttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagttgcggcacttcctgagccgtaacgaaaaggtttatcggaatgctggttggggtgaactttgggctgactgttgtgattttatgttTg                                                                                                                                                                                                                                                <  2:149342‑M2/2‑1 (MQ=255)                              atcgtttagacatggctattaaactttgatgttaaatgaatttaacaattagatcacgctgtgtaacctattaggttttttaatctgagccatagtgaattggccgttgagcttctagcagcaaatacctatagtggcggcacttcgtgtgccggaatgaaaagtgttatcggaatgtgtgtgctggtgaacttttggcttactgttgtgattttgggttTGCAATTGGTCTGCGAGTCAGACCATGGTAg                                                                                                                                                                                                                   <  1:106918‑M2/31‑1 (MQ=255)                                            gctattaaactttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCtttt                                                                                                                                                                                                     >  1:332553‑M2/207‑251 (MQ=255)                                                 taaactttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgattttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCa                                                                                                                                                                                                 <  1:288246‑M2/49‑1 (MQ=255)                                                    actttgatgttaaatgaatttaacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggcacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttaaggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTc                                                                                                                                                                                             >  2:310190‑M2/199‑251 (MQ=255)                                                                    aatttaacaattagatcacactatgtaacctattagtttttttaagctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCctg                                                                                                                                                                             <  1:175226‑M2/69‑1 (MQ=255)                                                                    aatttaacaattagatcacactatgtaacctattagattttttaatctgagcaattataaattgtccgttgcgcttctaccagcacatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATTGTAGCAAAACTACCTTTTTTTACTTCCTGTACATTTACCctg                                                                                                                                                                             >  2:298035‑M2/183‑251 (MQ=255)                                                                        taacaattagatcacactatgtaacctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCctgtct                                                                                                                                                                          <  2:71260‑M2/72‑1 (MQ=255)                                                                                                 ctattagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGc                                                                                                                                                >  2:322741‑M2/154‑251 (MQ=255)                                                                                                   attagtttttttaatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGcc                                                                                                                                               <  1:166963‑M2/99‑1 (MQ=255)                                                                                                              taatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGc                                                                                                                                   >  1:293488‑M2/141‑251 (MQ=255)                                                                                                               aatctgagccattataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCt                                                                                                                                  <  2:101286‑M2/112‑1 (MQ=255)                                                                                                                           tataaattgtccgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCtt                                                                                                                      >  1:276149‑M2/128‑251 (MQ=255)                                                                                                                                      cgttgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGaa                                                                                                           <  1:310190‑M2/135‑1 (MQ=255)                                                                                                                                         tgagcttctaccagcaaatacctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTCATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGTGATCGCGAAtt                                                                                                         >  1:105315‑M2/114‑250 (MQ=255)                                                                                                                                                ctaccagcaaatacctatagtggcggcacttccagaaccgaaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTAGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGACTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATcc                                                                                                 >  1:128487‑M2/107‑251 (MQ=255)                                                                                                                                                             cctatagtggcggcacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCAtct                                                                                    <  1:356037‑M2/158‑1 (MQ=255)                                                                                                                                                                         gcacttcctgagccggaacgaaaagttttataggaatgcgtgttctggtgaatttttggcttacggttgtgatgttgtgttTGCAATTGGTATGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTc                                                                          >  1:352479‑M2/82‑249 (MQ=255)                                                                                                                                                                          cacttcctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgctgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGt                                                                       >  2:39280‑M2/81‑251 (MQ=255)                                                                                                                                                                           acttcctgagccggaacgaaaagttttatcggaattcttgttctggtgcacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTTCGATTCAGACCATGGCAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTACTGTAGCACCGCCTAATTGCGGTGCTTTTTTTTAACTTGCGATCGCGAATTACTGATCCAGTTCGAACATCTCTTTCACGTCAGt                                                                       >  1:183786‑M2/80‑250 (MQ=255)                                                                                                                                                                           acttcctgagccggaacgaaaaggtttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGt                                                                       >  2:310812‑M2/80‑250 (MQ=255)                                                                                                                                                                               cctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTGCGACCATCTCTTTCACGGCAGTACgg                                                                   <  2:140080‑M2/175‑1 (MQ=255)                                                                                                                                                                               cctgagccggaacgaaaagttttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGGCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTTATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACgg                                                                   <  2:206067‑M2/175‑1 (MQ=255)                                                                                                                                                                                                    ttatcggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCAt                                             >  1:122174‑M2/55‑251 (MQ=255)                                                                                                                                                                                                        cggaatgcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGc                                         >  1:219846‑M2/51‑251 (MQ=255)                                                                                                                                                                                                              gcgtgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCtt                                    <  1:136735‑M2/206‑1 (MQ=255)                                                                                                                                                                                                                 tgttctggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTtat                                 >  2:57136‑M2/42‑250 (MQ=255)                                                                                                                                                                                                                       ggtgaacttttggcttacggttgtgatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAAt                          >  1:280135‑M2/36‑251 (MQ=255)                                                                                                                                                                                                                                               gatgttgtgttTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTATCGTTATCAGTCtg  <  1:310812‑M2/240‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                GTAAGGCATTTAGCGGTGGTAATCGTTTAGACATGGCTATTAAACTTTGATGTTAAATGAATTTAACAATTAGATCACACTATGTAACCTATTAGTTTTTTTAATCTGAGCCATTATAAATTGTCCGTTGAGCTTCTACCAGCAAATACCTATAGTGGCGGCACTTCCTGAGCCGGAACGAAAAGTTTTATCGGAATGCGTGTTCTGGTGAACTTTTGGCTTACGGTTGTGATGTTGTGTTGTTGTGTTTGCAATTGGTCTGCGATTCAGACCATGGTAGCAAAGCTACCTTTTTTCACTTCCTGTACATTTACCCTGTCTGTCCATAGTGATTAATGTAGCACCGCCTAATTGCGGTGCTTTTTTTTACCTTGCGATCGCGAATTACTGATCCAGTTCGACCATCTCTTTCACGTCAGTACGGTTGATCTGCTGTTTGTTGCCATTAGCGTCTTTATACGAAATCATACCGGTATCGTTATCAGTCTG  >  NC_000913/2942534‑2943022

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑