(go
to KOMUGI Home) (go
to WIS List) (go
to NO.75 Contents)
Results
Comparative analysis of mitochondrial DNA
Patterns of EcoRI and BamHI restriction fragments
are shown in
Fig.1. According
to the obtained data mt DNA of the alloplasmic wheat
(cereale)-Lt62 was similar to that of maternal
rye var. Vyatka. Varieties of rye had shown different patterns of mt
DNA (Fig.1A).
Differences between Lt62 and var. Vyatka concerned only the glow
intensity of some fragments, but not their quantity. At least the
majority of plants of this alloplasmic wheat is supposed to contain
mt DNA of the maternal rye variety. Two selected lines 92 and 96 from
this alloplasmic wheat Lt62 were investigated for heterogeneity in
organelle DNA. The data had shown that the line 92 contained the
typical rye mt DNA of Vyatka, while the line 96 differed from wheat
and rye.
Mt DNA of the 555 hybrid was similar neither to wheat nor rye mt DNA.
It had both unique and similar parental fragments. More probable
explanation of these data is biparental inheritance of mt DNA and as
a result of this the development of mt DNA diversity among hybrid
plants, which contained molecules of both parents and recombinant
ones.
The lines isolated from hybrids (cereale)-Lt62 x T.
aestivum cv. Leningradka had differential mt DNA. L1 and L2 lines
were similar to each other, but had mt DNA different from that of
rye, wheat and hybrid 555. L3 lines had the same mt DNA as maternal
lines as is shown by both restriction enzymes.
All three lines isolated from hybrid (cereale)-Lt62 x T.
aestivum cv. Ledovka contained mt DNA pattern identical with male
but not female parents.
Comparative analysis of chloroplast DNA
Patterns of restriction fragments of ct DNA are shown in
Fig.
2. Ct DNA of
alloplasmic wheat Lt62 with S. cereale cytoplasm was
not different in number and distribution of their fragments from its
female parent rye variety Vyatka. However, the lines isolated form
this alloplasmic wheat differed from each other. The line 92 had rye
ct DNA, the line 96 had ct DNA similar to wheat.
Ct DNA of the hybrid 555 as
its mt DNA was unique
and probably a mixture of both parental DNAs and/or alternated
molecules.
Three lines originated from hybrid (cereale)-Lt62 x Ledovka
had ct DNA of wheat type (EcoRI and BamHI), but
three lines originated from hybrid (cereale)-Lt62 x
Leningradka had ct DNA of an alternated type at least as is shown by
BamHI.
<--Back |-->Next
(go
to KOMUGI Home) (go
to WIS List) (go
to NO.75 Contents)