Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,995,620	IS5 (–) +4 bp	intergenic (‑261/‑32)	uvrY ← / → yecU	DNA‑binding transcriptional activator UvrY/protein YecU

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1995620 =	0 (0.000)	5 (0.500)	4/318	0.6	100%	intergenic (‑261/‑35)	uvrY/yecU	DNA‑binding transcriptional activator UvrY/protein YecU
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1995623	0 (0.000)	8 (0.830)	5/308	0.4	100%	intergenic (‑264/‑32)	uvrY/yecU	DNA‑binding transcriptional activator UvrY/protein YecU
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region

TTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACC  >  NC_000913/2101500‑2101992                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    aatagggataacgctactgatagttagtcattcgtatattagcggtttttattgcaactttgaaacgttaaaaatggtgggtattcgatttttctctcgtttttgtcaattcgattgtccacagttatggtcacgcccgtcaccaacggcttaccggatcgtttcacagtaagccctacgatgaaatgtgacaaaaattgactttattcagcaaaaatgaaaatcagcccgctgaatatgtaacattaagg                                                                                                                                                                                                                                                    <  1:32740‑M2/2‑1 (MQ=255)                                  aaTGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATGTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGc                                                                                                                                                                                                                   <  1:69766/251‑1 (MQ=255)                                                 aaGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCAAAGTACATAAAGTAACCTTGATCGCAGCCATTTACCCGCAAAAAATCTGAACCACGTACCAGCATAAATGATCCTGCGCAACATTCAACAACCGCATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGtcatttc                                                                                                                                                                                                    >  1:235919/1‑251 (MQ=255)                                                          ctttgaaacgttaaaaatggtgggtattcgagttttctctcgtttttgtcaattcgattgacaacagttatggtcacgaccgtaaccaacagcttaccggctcgtttcacagtaagccctacgatgcaatgcgacaaaaattgactttattcagcaaaaatgaaaatcagcccgctgaatatgtaacattaaGGAAGGTGCGAATAAGCGGGAAAATTCTTCTAGGCTGCATCAGTAATTTCATttcttc                                                                                                                                                                                            >  1:163069‑M2/193‑250 (MQ=255)                                                                            nagccagGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAtttttt                                                                                                                                                                         >  1:59640/2‑251 (MQ=255)                                                                             agccagGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTtc                                                                                                                                                                        <  1:185175/251‑1 (MQ=255)                                                                                   gCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGcc                                                                                                                                                                                 >  1:176338/1‑236 (MQ=255)                                                                                                  caGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCCCAAAATCTGAAAAACGTACCAGCATAAATGCTCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGCATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATc                                                                                                                                                    >  1:31476/1‑250 (MQ=255)                                                                                                             cTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACAGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAttt                                                                                                                                           >  1:135232/1‑248 (MQ=255)                                                                                                                          aaaGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAAGTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTATACCGTTTCTTCGc                                                                                                                            <  1:190869/250‑1 (MQ=255)                                                                                                                                 ccTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTa                                                                                                                      >  1:77936/1‑249 (MQ=255)                                                                                                                                      ntCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGa                                                                                                                                                                               >  1:136512/2‑187 (MQ=255)                                                                                                                                              accaacggcttaccggatcgtttcacagtaagcccttcgatgaaatgtgacaaaaattgactttattcagcaaaaatgaaactcagcccgctgcatatgacacattacGGATGGTGCGAATAAGCGGGGAATTTCTTCTCGGCTGACTCAGTCATTTCATTTCTTAATGTTTGAGCAGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATcccc                                                                                                         >  1:75883‑M2/109‑249 (MQ=255)                                                                                                                                                      cGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAgn                                                                                               <  1:145505/251‑2 (MQ=255)                                                                                                                                                            aaaTCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAtc                                                                                         <  1:73435/251‑1 (MQ=255)                                                                                                                                                                       nnnnnnnnnnaTNNNNNATCNTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGa                                                                                                                                                                               <  2:136512/144‑1 (MQ=25)                                                                                                                                                                                        aTCCCGCGCACCAATCAACAACCGTATCAGAATAGATACGTTCTTTAGGAATGTTTGTTTTATTAAGTAAGGGGCGAATAAGCGGCGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATTTTTGAGc                                                                                                                                                                                  <  1:118892/134‑1 (MQ=25)                                                                                                                                                                                         aatgtgacaaaaattgactttattcagcaaaaatgaaaatcagcccgctgaatatgtaacattaaGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCAGTTGGTCCGcc                                                            <  1:159701‑M2/186‑1 (MQ=255)                                                                                                                                                                                                            ttattcagcaaaaatgaaaatcagcccgctgaatatgtaacattaaGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCagn                                          <  1:57161‑M2/204‑2 (MQ=255)                                                                                                                                                                                                                          gATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGCGCGAATAAGCGGGGAAATTATTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTATAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGcc                           >  1:175005/1‑251 (MQ=255)                                                                                                                                                                                                                                  agcccgctgaatatgtaacattaaGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCGTTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGgtn                   <  1:21349‑M2/227‑2 (MQ=255)                                                                                                                                                                                                                                    ttAGGAATTTTTGTTTTATTAAGTAAGGTGCGAATAAGCTGTGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTGGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCATCCTATGTAGACCGTTTCTTCGCCAGTTAAGGCGTTTGCCCCAGTGTTTAGTGAGACCTCTCCCACTGACGTATCATTTGGTCCGCCCAAAACAGGTTGGCCAGCTTGAATAACATCGCCAGTTGGt                    <  1:222044/248‑1 (MQ=11)                                                                                                                                                                                                                                    cccgctgaatatgtaacattaaGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTatn                                                                        <  1:59049‑M2/174‑2 (MQ=255)                                                                                                                                                                                                                                         aaTTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTAAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCCGCCTATTTAGACCGTTTCTTCGCCCTTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGTCGTATCATTTGGTCCGCCCGAAACAGGATGGCCAGCGTGAATCACATCGCCACTTGGTTATCGttt            >  1:123157/1‑251 (MQ=11)                                                                                                                                                                                                                                                   acattaaGGAAGGTGCGAATAAGCGGGGAAATTCGTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGGAAATGCCTTGAATCAGCCTATTTATACCGGTTCGTCGCCATTTAATGCGTTATCCCCAGTTTTGAGTGAGATCTCTCCCACTGACGTATCATTTGGGCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAcc  <  1:32628‑M2/244‑1 (MQ=255)                                                                                                                                                                                                                                                          |                                                                                                                                                                                                                                                    TTGAAAAAAAACTTCGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAACCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAACC  >  NC_000913/2101500‑2101992

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.