Predicted mutation
evidence seq id position mutation annotation gene description
JC JC NC_000913 3,262,484 IS5 (+) +4 bp coding (1174‑1177/1209 nt) tdcD ← propionate kinase

New junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 2288919 =NA (NA)13 (1.120) 7/302 0.3 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 3262484 = 0 (0.000)coding (1177/1209 nt) tdcD propionate kinase
* ? NC_000913 = 2290113NA (NA)31 (2.660) 21/304 0.0 100% noncoding (1/1195 nt) IS5 repeat region
?NC_000913 = 3262487 0 (0.000)coding (1174/1209 nt) tdcD propionate kinase

GTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2289870‑2290113
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGGACTGGAAAAACGAAATTAATGTCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGC  <  NC_000913/3262487‑3262267
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
GTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGC                                                                                                                                                                                                                         >  1:84274/1‑250
GTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGCGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACACGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATATAATATCAGGGACTTGTTCGCACCTTCCTTAGGC                                                                                                                                                                                                                         >  1:272663/1‑250
               GGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGGTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTCGGCAAAATTAACGCGCCCG                                                                                                                                                                                                         >  1:39996/1‑251
                         CATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACCAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAATTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGAAAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAG                                                                                                                                                                                                                     >  1:102465/1‑229
                                        GGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGAACAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGGTGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGAATAATTTAGTTGA                                                                                                                                                                                  >  1:203983/1‑249
                                         GTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATATCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCAAGATGCCAAACATGATCTACTATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCC                                                                                                                                                                                                           >  1:181512/1‑223
                                                  CTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAG                                                                                                                                                                        >  1:139885/1‑249
                                                                 CATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCTCTCCTTCCTTAGGCAAAGTTAACTCTCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATt                                                                                                                                                       <  1:39269/251‑2
                                                                           CATGGCCGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGAGCTTACTGCTGCATTCAATGTCGGCGAACGTAAGTTGATGCCTCATGCTGAACCCTGTTCTATGGCTCCAGCTGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGAT                                                                                                                                              >  1:42865/1‑250
                                                                                                     CAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTTTTCTATTGCTCCAGATTACAAACATGAGCTCAGATCAGTGACTTGTTCTCACCTTCCTTAGGCAAAGTTAACGCGCCCTCAGAATTTGCATAATTTAGTTAAAGTAGTGTAGAGAGATTATTTGTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGac                                                                                                                   <  1:252527/251‑3
                                                                                                          AAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGC                                                                                                              >  1:133754/1‑251
                                                                                                                CTTTTCTGGTCTGACGGCGCTTCCTGCTGACATCCCCGTCGGCGAAGGTAAGTTGATGAATCATGCTGAACCCTGTTCTATGGCTCCAGATGACAAACATGATATCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGAATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTG                                                                                                                                          >  1:233900/1‑217
                                                                                                                CTTTTCTGGTCTGACGGCGATTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTCGAGAGATTATTTTTCATGAAGGTAGCTATTGATACC                                                                                                                                     >  1:4016/1‑222
                                                                                                                     CTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCT                                                                                                   >  1:15198/1‑251
                                                                                                                        nTCTGAAGGCGATTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTT                                                                                                 >  1:69404/2‑250
                                                                                                                            nACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGG                                                                                            >  1:162294/2‑251
                                                                                                                            GACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTG                                                                                                      <  1:186887/241‑1
                                                                                                                                  GCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGCCTCATGATGAACCCTGTTCTATGGCTACAGATGACAACCATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGG                                                                                       >  1:39255/1‑250
                                                                                                                                                        GGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGGACTGGAAAAACGAAATTAATGTC                                                                >  1:78878/1‑251
                                                                                                                                                               GTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTGAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTAGTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATG                                                                                                           <  1:39872/201‑1
                                                                                                                                                                                GATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGGACTGGAAAAACGAA                                                                         >  1:76618/1‑218
                                                                                                                                                                                    AACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAAGTTAGTTGAAGGATTGTAGAGAGAGTATGTTGCATGAAGGTATATATGGATACCATCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTA                                                                                          <  1:123916/197‑1
                                                                                                                                                                                      nCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGCTTATTTTTCATGACGGTAGATATTGATACCAGCGATA                                                                                                                              >  1:53110/2‑159
                                                                                                                                                                                                  GCTCCAGCTGAAAAACCTGATCTAATATCAGGGACTAGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATCATTTAGTTGACGTATTGTCGAGAGATTCTTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGGACTGGAAAAACGAAATTAATGTCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGAT                      >  1:235150/1‑251
                                                                                                                                                                                                     CCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGACTTTGCATAATTTAGTTGAAGTATTGTAGAGAGCTTCTTTTTCATGCAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTCAAGGTCCGGCCTGGCAAAACGAAATTAATGTCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGA                    >  1:108342/1‑250
                                                                                                                                                                                                                 CATGATCTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGGACTGTAAAAACGAAATTAATGTCCTCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCC       <  1:227364/251‑1
                                                                                                                                                                                                                       nTCATATCAGGGACTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGGACTGGAAAAACGAAATTAATG                                                                  >  1:69885/2‑186
                                                                                                                                                                                                                                    CTTGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGTAGAATTTGCATAAGTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGG                                                                                                     <  1:151798/138‑1
                                                                                                                                                                                                                                      TGTTCGCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATn                                                                                                           <  1:28411/130‑2
                                                                                                                                                                                                                                           nCACCTTCCTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGGACTGGAAAAACGAAATTAATGTCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGC  >  1:68483/2‑230
                                                                                                                                                                                                                                                   CTTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTGGTTGAAGTATTGTAGCGCGATTATTTTTCATGAAGGTAGATATTGATCCCGGCGATAGGCTGTACGCCGACGCATGGCTTGGCTTTAAtgctacg                                                                                        >  1:158627/1‑129
                                                                                                                                                                                                                                                                                                                                                                                                                                                                                 
GTTTCCAGCGGATAAGGTCGCCGGCCATTACCAGCCTTGGGGTAAAACGGCTCGATGACTTCCACCATGTTTTGCCATGGCAGAATCTGCTCCATGCGGGACAAGAAAATCTCTTTTCTGGTCTGACGGCGCTTACTGCTGAATTCACTGTCGGCGAAGGTAAGTTGATGACTCATGATGAACCCTGTTCTATGGCTCCAGATGACAAACATGATCTCATATCAGGGACTTGTTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  >  NC_000913/2289870‑2290113
‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑TTAGGCAAAGTTAACGCGCCCGCAGAATTTGCATAATTTAGTTGAAGTATTGTAGAGAGATTATTTTTCATGAAGGTAGATATTGATACCAGCGATAAGCTGTACGCCGACGCATGGCTTGGCTTTAAAGGTACGGACTGGAAAAACGAAATTAATGTCCGCGATTTTATTCAACATAACTATACACCGTATGAAGGCGATGAATCTTTCCTCGCCGAAGC  <  NC_000913/3262487‑3262267

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: 
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.