Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	3,262,484	IS5 (+) +4 bp	coding (1174‑1177/1209 nt)	tdcD ←	propionate kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2288919 =	NA (NA)	13 (1.120)	7/302	0.3	100%	noncoding (1195/1195 nt)	IS5	repeat region
	?	NC_000913	3262484 =	0 (0.000)					coding (1177/1209 nt)	tdcD	propionate kinase
*	?	NC_000913	= 2290113	NA (NA)	31 (2.660)	21/304	0.0	100%	noncoding (1/1195 nt)	IS5	repeat region
	?	NC_000913	= 3262487	0 (0.000)					coding (1174/1209 nt)	tdcD	propionate kinase

GCTGAAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2289158‑2288919 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACTGGAAACAATTCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATCAATCTCTAAGCCTAATACAGCCAAATGTTCCATGACCAGACGACGAATTAAGCTTGAATTCTCTCCTATTCCGCCGGTG  >  NC_000913/3262484‑3262692                                                                                                                                                                                                                                                                                                                                                                                                                                                                   nCTGAAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATGAATG                                                                                                                                                                                                        >  1:172402/2‑251                                    nGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTCAAAACTGGGGATAACGCCTTAAATGGCGACGAAACGGTCTAAATAGGCTGATTCACGGCATTTACGGGAGAAAAAATCGGCTCAAACATGACGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATGACTGGCATCCAAAGC                                                                                                                                                                                             >  1:34368/2‑227                                                             nCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATGAATGGCATCCAAAGC                                                                                                                                                                                             >  1:33648/2‑202                                                              nAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCAT                                                                                                                                           >  1:52493/2‑251                                                                       CGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTATAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATGAATGGCATCCAAAGAAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACA                                                                                                                                                         >  1:251367/1‑228                                                                                                            CGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCACACCTTTCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGag                                                                                                                                              <  1:267483/202‑3                                                                                                               CTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTT                                                                                                                                                                           <  1:17782/170‑1                                                                                                                                  GTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCTGAATAACGGCACAAATGACACGCGCATTTTCACTGGAAACAATTCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATCAATCTCTA                                                                         >  1:62844/1‑249                                                                                                                                                             ACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAAGGACACGCGCATTTTCACTGGAAACAATTCGCTCACCACAGGAGGTAGAGCGATTATTCATTTCTGTATCAATCTCTAAGCCTAATACAGCCAAATGTTCCATGACC                                            <  1:108506/251‑1                                                                                                                                                              CGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTA                                                                                                                                                                          >  1:212126/1‑124                                                                                                                                                                             GCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCTCCTTCCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACTGGA                                                                                                                                 <  1:5565/150‑1                                                                                                                                                                                                        GAGTCAGCCGAGAAGAATTTACCCGATTATTCGCACCTTCCCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACACATGAAACGCGCATTTTCACTGGAAACAATTCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATCAATCTCTAAGCCTAATACAGCCAAATGTTCCATGACCAGACGACGAATTAAGCTTGAATTCTCTCCTATTCCGCCGGTG  >  1:211892/1‑250                                                                                                                                                                                                                             nnnnnntannnnncCCNTCCCTAAATGAATGGCATCCAAAGCATTCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACTGGAAACAATTCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATC                                                                                 <  2:172402/136‑1                                                                                                                                                                                                                                                                                                                                                                                                                                                                   GCTGAAAAACGATAACCAACTGGCGATGTTATTCACGCTGGCCAACCTGTTTCGGGCGGACCAAATGATACGTCAGTGGGAGAGATCTCACTAAAAACTGGGGATAACGCCTTAAATGGCGAAGAAACGGTCTAAATAGGCTGATTCAAGGCATTTACGGGAGAAAAAATCGGCTCAAACATGAAGAAATGAAATGACTGAGTCAGCCGAGAAGAATTTCCCCGCTTATTCGCACCTTCC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2289158‑2288919 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑cCTAAATGAATGGCATCCAAAGCAATCATTTTTTCTTCGTTAGTCGGAATAACGGCACAAATGACACGCGCATTTTCACTGGAAACAATTCGCTCACCACAGGAGTTAGAGCGATTATTCATTTCTGTATCAATCTCTAAGCCTAATACAGCCAAATGTTCCATGACCAGACGACGAATTAAGCTTGAATTCTCTCCTATTCCGCCGGTG  >  NC_000913/3262484‑3262692

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.