Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC JC	NC_000913	1,293,268	IS5 (–) +4 bp	intergenic (‑346/‑256)	hns ← / → tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	1293268 =	0 (0.000)	22 (1.260)	16/290	0.1	100%	intergenic (‑346/‑259)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	= 2290113	NA (NA)					noncoding (1/1195 nt)	IS5	repeat region
*	?	NC_000913	= 1293271	0 (0.000)	20 (1.140)	13/292	0.2	100%	intergenic (‑349/‑256)	hns/tdk	DNA‑binding transcriptional dual regulator H‑NS/thymidine/deoxyuridine kinase
	?	NC_000913	2101749 =	NA (NA)					noncoding (1195/1195 nt)	IS5	repeat region

CGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAC  >  NC_000913/2101514‑2101991                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   cGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaaggaagg                                                                                                                                                                                                                                               >  1:180260/1‑241 (MQ=255)                            gCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAGTATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACGTTCGTTAGGAATTTTTGTTTTATTaagg                                                                                                                                                                                                                                                   <  1:360091/210‑1 (MQ=255)                                 aaaaGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTAAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAg                                                                                                                                                                                                          >  1:59810/1‑246 (MQ=255)                                                    aGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAg                                                                                                                                                                                  <  1:248676/251‑1 (MQ=255)                                                     gTCTGACACCAGCCAGGCTAAGCTTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAATCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCAGAAATGATCCTGCGCCGCAATCATCAA‑CCGTATCATAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaaggaag                                                                                                                                                                                                                                                <  1:421430/188‑1 (MQ=255)                                                     gTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGCTCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCgg                                                                                                                                                                                                                   >  1:180518/1‑217 (MQ=255)                                                     gTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGc                                                                                                                                                                                 <  1:372/251‑1 (MQ=255)                                                                           ccTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGc                                                                                                                                                           >  1:261478/1‑251 (MQ=255)                                                                                      ggTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAT‑CCGTATCAGAATAGATACTTTCTTGAGGAATGTTTGGTTTATTAAGGAAGGTGCGAATAAGCGGGGATATTCTTCTCGGCTTACGCAGTCATCTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                                                                                                                                <  1:410517/251‑1 (MQ=37)                                                                                      ggTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGc                                                                                                                                                <  1:153396/251‑1 (MQ=255)                                                                                                 tCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGTGGAAATTCTTCTCAGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAg                                                                                                                                        <  1:163227/248‑1 (MQ=255)                                                                                                                   ccTTGATCGAAGCCATTTACACGCACAAAATCTGAAACACGAACCAGCATCAATGATCCTGCGCAAAAAT‑AACAAACAGTATAAGAATAGATACTTTCTTTCGGACTTTTTGTTTTCTTAAGGAAGGTGCGACTAAGCg                                                                                                                                                                                                                                   >  1:208007/1‑139 (MQ=255)                                                                                                                    cTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAC‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAgg                                                                                                                  >  1:152180/1‑251 (MQ=255)                                                                                                                    cTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAAGCAACAA‑CCGTATCAGAATAGAGTCGTTCTTTAGGAATTTTTGTTTTATGAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGTCTGACGCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTg                                                                                                                                                       <  1:320538/214‑1 (MQ=25)                                                                                                                            aaGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAg                                                                                                                                                                                                                                     <  1:20056/128‑1 (MQ=255)                                                                                                                              gCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCg                                                                                                                            <  1:336224/231‑1 (MQ=255)                                                                                                                              gCCATTTACACGCACAAAAGCTGAAAAACGTACCAGCATAAAGGATCCTGCTCACCAAGCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTaaggaag                                                                                                                                                                                                                                                <  1:434672/115‑1 (MQ=255)                                                                                                                                                            naCCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCtct                                                                                                                                            >  1:279085/2‑183 (MQ=255)                                                                                                                                                                  naTAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGATTCAGTCATTTCATTTTTTCAGGTTTGAGCCGATTTTTTCTCCCGTAATTGCCTTGAATCAGCCTCTTTAGACCTTTTCTTGGCAATTTAAGGCGTTATCCCCCGTTTTTAGGGAGATTTCTCCCatgaacgaaccatt                                                                    >  1:114041/2‑238 (MQ=18)                                                                                                                                                                          aTCCTGCGCCCCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGAAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGc                                                                                                                                                                                                                  <  1:234659/101‑1 (MQ=255)                                                                                                                                                                          aTCCTGCGCACCAATCAACAA‑ACGTATCAGAATAGATTCTTTCTTTAGGCCTTTTTGTTTTCTCACGGAAGCTGCGACTAAGCGGGGAAATCCTTCTCCGCTGACCCAGTCCTTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGACTCAGCCTATTTAGACCGTTTCTTCGCCCTTTAAGGCGTTATCCCCAGCTTTTAGTGAGAtctctc                                                                                    >  1:184435/1‑227 (MQ=17)                                                                                                                                                                                  cANCAATCNACAA‑NCGTATCAGAATAGATNCTTTCTTTAGGAATTTTTGNNTNATTaangaagg                                                                                                                                                                                                                                               <  2:180260/64‑1 (MQ=255)                                                                                                                                                                                     cAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAGTTTTTGTTTTATTAAGGAAGGTGCGACTAAGAGGGGAAATTCTTCTCGCCTGACTCAGTCATTTCATTTCATCATGTTTGAGCCGATTTTTTCTAACGTAAATGCCTTGAATCAGCCTATTTAGCCCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGt                                                 >  1:309132/1‑251 (MQ=17)                                                                                                                                                                                                      cANNNNNGATNCTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTAt                                                                                                                                            <  2:279085/144‑1 (MQ=255)                                                                                                                                                                                                        gAATAGATACTTTCTTTAGGAATTTTTGTTTTCTTAAGGAAGGTGCGAATAAGAGGGGACGTTCTTCTCgg                                                                                                                                                                                                                   >  1:239814/1‑71 (MQ=255)                                                                                                                                                                                                            ngNTANTTTCTNTAGGNATTTTTGTTTTATTANGGAAGGTGCGAATAAGCGGNNANAGTCTNCTCgg                                                                                                                                                                                                                   <  2:180518/66‑1 (MQ=21)                                                                                                                                                                                                                            aaTTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGc                                                                                                                                                                                                                  <  1:364642/52‑1 (MQ=255)                                                                                                                                                                                                                                      ttattaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGAGCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACAGCGCCAGTTGGTTATCGTTTTTCAGCAAc  <  1:137947/250‑1 (MQ=14)                                                                                                                                                                                                                                      ttattaAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGAGTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGAc                                                                                                                                      <  1:250348/118‑1 (MQ=18)                                                                                                                                                                                                                                             |                                                                                                                                                                                                                                                   CGATTGTCATGATGAGCATAATGTATCGCATGAAAAGCGGGAACATAATGAAGTCTGACACCAGCCAGGCTAAGCCTCAAGCACAGGTCAATATCTTCACAGTACATAAAGTAACCTTGATCGAAGCCATTTACACGCACAAAATCTGAAAAACGTACCAGCATAAATGATCCTGCGCACCAATCAACAA‑CCGTATCAGAATAGATACTTTCTTTAGGAATTTTTGTTTTATTAAGGAAGGTGCGAATAAGCGGGGAAATTCTTCTCGGCTGACTCAGTCATTTCATTTCTTCATGTTTGAGCCGATTTTTTCTCCCGTAAATGCCTTGAATCAGCCTATTTAGACCGTTTCTTCGCCATTTAAGGCGTTATCCCCAGTTTTTAGTGAGATCTCTCCCACTGACGTATCATTTGGTCCGCCCGAAACAGGTTGGCCAGCGTGAATAACATCGCCAGTTGGTTATCGTTTTTCAGCAAC  >  NC_000913/2101514‑2101991

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 16 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 39 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.