Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	2,092,996	(TGGC)4→3	coding (599‑602/1071 nt)	hisC →	histidinol‑phosphate aminotransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2092986 =	2 (0.110)	8 (0.500)	4/264	1.3	85.1%	coding (589/1071 nt)	hisC	histidinol‑phosphate aminotransferase
	?	NC_000913	= 2092981	1 (0.060)					coding (584/1071 nt)	hisC	histidinol‑phosphate aminotransferase

CAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCG  >  NC_000913/2092742‑2093226                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 cAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCCCCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATAGCGAGTTTTGCCCGCAGGCATCgctggctn                                                                                                                                                                                                                                               <  1:112201/248‑2 (MQ=255)                                                                    cttacagggcatttccgacaagctggacggcgtaaaagtggtttatgtttgcagccccaataacccgaccgggcaactgatcaatccgcaggattttcgcaccctgctggagttaacccgcggtaaggcgattgtggttgccgatgaagcctatatcgagttttgcccgcaggcatcGCTGGCTGGCTGGCGGAATATCCGCACCTGGc                                                                                                                                                                                                                   >  1:383291‑M1/178‑209 (MQ=255)                                                                          gtgcatttccgacaagctggacggcgtaaaagtggtttatgtttgcagcgccaataacccgaccgggcaactgatcaatccgcaggatattcgcaccctgctggagttaacccgcggtaaggcgattgtggttgccgatgaagcctatatcgagttttgcccgcaggcatcGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGTTTTTGCTCTGGCGGGGCt                                                                                                                                                                         <  1:428205‑M1/74‑1 (MQ=255)                                                                                                   ggaaaagtggtttatgtttgcagccccaataacccgaccgggcaactgatcaatccgcaggattttcgcaccctgctggagttaacccgcggtaaggcgattgtggttgccgatgaagcctatatcgagttttgcccgcaggcatcGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACgaaga                                                                                                                                           <  1:424540‑M1/104‑1 (MQ=255)                                                                                                                                                            caggatttgcgcaccctgctggtgttaacccgcggtaaggcgattgtggttgccgatgaagcctatatcgagttttgcccgcaggcatcGCTGGCTGGCTGGCGGAATATCCGCACCTGTCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGcc                                                                                 >  1:322‑M1/90‑251 (MQ=255)                                                                                                                                                                      gcaccctgctggagttcaccagcggtaaggcgcttgtggttgccgatgaagcctatatcgagttttgcccgcaggcatcGCTGGCTGGCTGGCGGAATATCCGCCCCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAAAGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCgg                                                                       <  1:345143‑M1/172‑1 (MQ=255)                                                                                                                                                                       caccctgctggagttaacccgcggtaaggcgattgtggttgccgatgaagcctatatcgagttttgcccgcaggcatcGCTGGCTGGCTGGCGGAAGATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTgcn                                                                                                                                                                 >  1:49903‑M1/79‑159 (MQ=255)                                                                                                                                                                                                         gtggttgccgatgaagcctatatcgagttttgcccgcaggcatcGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTATGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTTATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATg                                    >  1:433588‑M1/45‑251 (MQ=255)                                                                                                                                                                                                                              aGTTTTGCCCGCAGGAATC‑‑‑‑GCTGGCTGGCTGGCGGAATATACGCACCTGGCTATTTTACGCACACTGTCGACAGCTTTTGCTCTGGCGGGGCTTCGTTGCgg                                                                                                                                                                >  1:438179/1‑102 (MQ=255)                                                                                                                                                                                                                                           ngcaggcatcGCTGGCTGCCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAAcgcg  <  1:275552‑M1/241‑1 (MQ=255)                                                                                                                                                                                                                                                     |                                                                                                                                                                                                                                                 CAGCGCCGAAACGATTGGCGTCGAGTGCCGCACAGTGCCGACGCTGGACAACTGGCAACTGGACTTACAGGGCATTTCCGACAAGCTGGACGGCGTAAAAGTGGTTTATGTTTGCAGCCCCAATAACCCGACCGGGCAACTGATCAATCCGCAGGATTTTCGCACCCTGCTGGAGTTAACCCGCGGTAAGGCGATTGTGGTTGCCGATGAAGCCTATATCGAGTTTTGCCCGCAGGCATCGCTGGCTGGCTGGCTGGCGGAATATCCGCACCTGGCTATTTTACGCACACTGTCGAAAGCTTTTGCTCTGGCGGGGCTTCGTTGCGGATTTACGCTGGCAAACGAAGAAGTCATCAACCTGCTGATGAAAGTGATCGCCCCCTACCCGCTCTCGACGCCGGTTGCCGACATTGCGGCCCAGGCGTTAAGCCCACAGGGAATCGTCGCCATGCGCGAACGGGTAGCGCAAATTATTGCAGAACGCG  >  NC_000913/2092742‑2093226

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 13 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.