Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
JC	NC_000913	2,092,996	(TGGC)4→3	coding (599‑602/1071 nt)	hisC →	histidinol‑phosphate aminotransferase

New junction evidence
		seq id	position	reads (cov)	reads (cov)	score	skew	freq	annotation	gene	product
*	?	NC_000913	2092986 =	2 (0.110)	8 (0.500)	4/264	1.3	85.1%	coding (589/1071 nt)	hisC	histidinol‑phosphate aminotransferase
	?	NC_000913	= 2092981	1 (0.060)					coding (584/1071 nt)	hisC	histidinol‑phosphate aminotransferase

CGCGTTCTGCAATAATTTGCGCTACCCGTTCGCGCATGGCGACGATTCCCTGTGGGCTTAACGCCTGGGCCGCAATGTCGGCAACCGGCGTCGAGAGCGGGTAGGGGGCGATCACTTTCATCAGCAGGTTGATGACTTCTTCGTTTGCCAGCGTAAATCCGCAACGAAGCCCCGCCAGAGCAAAAGCTTTCGACAGTGTGCGTAAAATAGCCAGGTGCGGATATTCCGCCAGCCAGCCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2093226‑2092986 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccagccagccagcGATGCCTGCGGGCAAAACTCGATATAGGCTTCATCGGCAACCACAATCGCCTTACCGCGGGTTAACTCCAGCAGGGTGCGAAAATCCTGCGGATTGATCAGTTGCCCGGTCGGGTTATTGGGGCTGCAAACATAAACCACTTTTACGCCGTCCAGCTTGTCGGAAATGCCCTGTAAG  <  NC_000913/2092981‑2092805                                                                                                                                                                                                                                                                                                                                                                                                                                    CGCGTTCTGCAATAATTTGCGCTACCCGTTCGCGCATGGCGACGATTCCCTGTGGGCTTAACGCCTGGGCCGCAATGTCGGCAACCGGCGTCGAGAGCGGGTAGGGGGCGATCACTTTCATCAGCAGGTTGATGACTTCTTCGTTTGCCAGCGTAAATCCGCAACGAAGCCCCGCCAGAGCAAAAGCTTTCGACAGTGTGCGTAAAATAGCCAGGTGCGGATATTCCGCCAGGCAGCCAGCGATGCCTGCn                                                                                                                                                                         <  1:275552/251‑2                                   CATGGCGACGATTCCCTGTGGGCTTAACGCCTGGGCCGCAATGTCGGCAACCGGCGTCGAGAGCGGGTAGGGGGCGATCACTTTCATAAGCAGGTTGATGACTTCTTCGTTTGCCAGCGTAAATCCGCAACGAAGCCCCGCCAGAGCATAAGCTTTCGACAGTGTGCGTAAAATAGCCAGGTGCGGATATTCCGCCAGCCAGCCAGCGATGCCTGCGGGCAAAACTCGATATAGGCTTCATCGGCAACCAC                                                                                                                                       >  1:433588/1‑251                                                                      CCGCAATGTCGGCAACCGGCGTCGAGAGCGGGTAGGGGGCGATCACTTTCATCAGCAGGTTGATGACTTCTTCTTTTGCCAGCGTAAATCCGCAACGAAGCCCCGCCAGAGCAAAAGCTTTCGACAGTGTGCGTAAAATAGCCAGGGGCGGATATTCCGCCAGCCAGCCAGCGATGCCTGCGGGCAAAACTCGATATAGGCTTCATCGGCAACCACAAGCGCCTTACCGCTGGTGAACTCCAGCAGGGTGC                                                                                                    <  1:345143/251‑1                                                                                GGCAACCGGCGTCGAGAGCGGGTAGGGGGCGATCACTTTCATCAGCAGGTTGATGACTTCTTCGTTTGCCAGCGTAAATCCGCAACGAAGCCCCGCCAGAGCAAAAGCTTTCGACAGTGTGCGTAAAATAGACAGGTGCGGATATTCCGCCAGCCAGCCAGCGATGCCTGCGGGCAAAACTCGATATAGGCTTCATCGGCAACCACAATCGCCTTACCGCGGGTTAACACCAGCAGGGTGCGCAAATCCTG                                                                                          >  1:322/1‑251                                                                                                                                          TCTTCGTTTGCCAGCGTAAATCCGCAACGAAGCCCCGCCAGAGCAAAAGCTTTCGACAGTGTGCGTAAAATAGCCAGGTGCGGATATTCCGCCAGCCAGCCAGCGATGCCTGCGGGCAAAACTCGATATAGGCTTCATCGGCAACCACAATCGCCTTACCGCGGGTTAACTCCAGCAGGGTGCGAAAATCCTGCGGATTGATCAGTTGCCCGGTCGGGTTATTGGGGCTGCAAACATAAACCACTTTTcc                                 <  1:424540/250‑3                                                                                                                                                                nGCAACGAAGCCCCGCCAGAGCAAAAGCTTTCGACAGTGTGCGTAAAATAGCCAGGTGCGGATCTTCCGCCAGCCAGCCAGCGATGCCTGCGGGCAAAACTCGATATAGGCTTCATCGGCAACCACAATCGCCTTACCGCGGGTTAACTCCAGCAGGGTG                                                                                                     >  1:49903/2‑160                                                                                                                                                                        AGCCCCGCCAGAGCAAAAACTTTCGACAGTGTGCGTAAAATAGCCAGGTGCGGATATTCCGCCAGCCAGCCAGCGATGCCTGCGGGCAAAACTCGATATAGGCTTCATCGGCAACCACAATCGCCTTACCGCGGGTTAACTCCAGCAGGGTGCGAATATCCTGCGGATTGATCAGTTGCCCGGTCGGGTTATTGGCGCTGCAAACATAAACCACTTTTACGCCGTCCAGCTTGTCGGAAATGCac        <  1:428205/245‑3                                                                                                                                                                                                                  GCCAGGTGCGGATATTCCGCCAGCCAGCCAGCGATGCCTGCGGGCAAAACTCGATATAGGCTTCATCGGCAACCACAATCGCCTTACCGCGGGTTAACTCCAGCAGGGTGCGAAAATCCTGCGGATTGATCAGTTGCCCGGTCGGGTTATTGGGGCTGCAAACATAAACCACTTTTACGCCGTCCAGCTTGTCGGAAATGCCCTGTAAG  >  1:383291/1‑209                                                                                                                                                                                                                                                                                                                                                                                                                                    CGCGTTCTGCAATAATTTGCGCTACCCGTTCGCGCATGGCGACGATTCCCTGTGGGCTTAACGCCTGGGCCGCAATGTCGGCAACCGGCGTCGAGAGCGGGTAGGGGGCGATCACTTTCATCAGCAGGTTGATGACTTCTTCGTTTGCCAGCGTAAATCCGCAACGAAGCCCCGCCAGAGCAAAAGCTTTCGACAGTGTGCGTAAAATAGCCAGGTGCGGATATTCCGCCAGCCAGCCAGC‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑  <  NC_000913/2093226‑2092986 ‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑‑gccagccagccagcGATGCCTGCGGGCAAAACTCGATATAGGCTTCATCGGCAACCACAATCGCCTTACCGCGGGTTAACTCCAGCAGGGTGCGAAAATCCTGCGGATTGATCAGTTGCCCGGTCGGGTTATTGGGGCTGCAAACATAAACCACTTTTACGCCGTCCAGCTTGTCGGAAATGCCCTGTAAG  <  NC_000913/2092981‑2092805

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 14 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 38 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑
Reads not counted as support for junction
read_name Not counted due to insufficient overlap past the breakpoint.
read_name Not counted due to not crossing MOB target site duplication.