breseq  version 0.39.0  
mutation predictions | marginal predictions | summary statistics | genome diff | command line log

Predicted mutations
evidence position mutation annotation gene description
RA 139,113 A→G F705L (TTC→CTC)  gcd ← quinoprotein glucose dehydrogenase
MC JC 257,908 Δ776 bp insB9[crl] insB9, insA9, [crl]
RA 470,131 C→T L421F (CTT→TTT)  mdlA → ABC transporter family protein MdlA
RA 557,635 A→T L36Q (CTG→CAG)  folD ← bifunctional methylenetetrahydrofolate dehydrogenase/methenyltetrahydrofolate cyclohydrolase
RA 638,047 C→T W176* (TGG→TAG)  dsbG ← protein sulfenic acid reductase and chaperone DsbG
JC JC 848,208 IS5 (–) +4 bp intergenic (‑204/+197) glnH ← / ← dps L‑glutamine ABC transporter periplasmic binding protein/DNA protection during starvation protein
JC JC 949,487 IS5 (–) +4 bp coding (79‑82/909 nt) ycaN ← putative DNA‑binding transcriptional regulator YcaN
JC JC 1,105,376 IS2 (–) +5 bp intergenic (+83/‑34) csgC → / → ymdA curlin chaperone/uncharacterized protein YmdA
RA 1,337,394 A→G S522G (AGC→GGC)  acnA → aconitate hydratase 1
MC JC 1,397,239 Δ3,340 bp IS5‑mediated ynaJ[abgT] ynaJ, uspE, fnr, ogt, [abgT]
RA 1,652,331 T→C intergenic (+2346/+397) ydfD → / ← ynfP lysis protein/protein YnfP
RA 1,744,309 T→G F285V (TTC→GTC)  mdtK → multidrug efflux pump MdtK
MC JC 1,756,291 Δ1,288 bp [pykF][lpp] [pykF], [lpp]
RA 1,964,511 C→A E180Y (GAG→TAT flhA ← flagellar biosynthesis protein FlhA
RA 1,964,513 C→A E180Y (GAG→TAT)  flhA ← flagellar biosynthesis protein FlhA
RA 1,992,640 T→G T60P (ACC→CCC)  pgsA ← CDP‑diacylglycerol‑‑glycerol‑3‑phosphate 3‑phosphatidyltransferase
RA 2,032,423 G→A V219V (GTC→GTT yedJ ← putative HD superfamily phosphohydrolase YedJ
RA 2,173,363 Δ2 bp intergenic (‑490/+918) gatD ← / ← gatB galactitol‑1‑phosphate 5‑dehydrogenase/galactitol‑specific PTS enzyme IIB component
JC JC 2,406,780 IS1 (–) +8 bp intergenic (‑139/‑774) lrhA ← / → alaA DNA‑binding transcriptional dual regulator LrhA/glutamate‑‑pyruvate aminotransferase AlaA
MC JC 2,558,699 Δ6,790 bp intZ[eutA] intZ, yffL, yffM, yffN, yffO, yffP, yffQ, yffR, yffS, [eutA]
RA 2,667,862 G→T intergenic (‑16/+144) hcaT ← / ← iroK putative 3‑phenylpropionate transporter/protein IroK
RA 2,867,455 G→A Q33* (CAG→TAG)  rpoS ← RNA polymerase sigma factor RpoS
RA 2,899,125 C→A V98V (GTG→GTT ygcU ← putative FAD‑containing dehydrogenase
RA 3,035,546 T→A T246S (ACG→TCG)  prfB ← peptide chain release factor RF2
RA 3,486,205 C→A T29K (ACG→AAG)  crp → DNA‑binding transcriptional dual regulator CRP
RA 3,560,455 +G intergenic (+589/+167) rtcR → / ← glpG DNA‑binding transcriptional activator RtcR/rhomboid protease GlpG
MC JC 3,625,589 Δ95,041 bp yhhJhokA 83 genes
RA 4,218,219 C→T Q371* (CAG→TAG)  aceA → isocitrate lyase
MC JC 4,284,307 Δ33 bp coding (564‑596/1650 nt) actP ← acetate/glycolate:cation symporter
RA 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
RA 4,549,066 G→T G87C (GGC→TGC)  fimH → type 1 fimbriae D‑mannose specific adhesin

Unassigned missing coverage evidence
   seq id start end size ←reads reads→ gene description
* * ÷ NC_000913 1978495 1979270–1978503 9–776 90 [0] [0] 129 insB5–insA5 insB5,insA5
* * ÷ NC_000913 3423812–3424234 3424465–3424238 5–654 38 [36] [36] 37 [rrlD] [rrlD]

Unassigned new junction evidence
  seq id position reads (cov) reads (cov) score skew freq annotation gene product
* ? NC_000913 1207790 =68 (0.650)53 (0.530) 41/556 1.0 45.4% coding (290/630 nt) ycfK protein StfP
?NC_000913 1209619 = 63 (0.640)intergenic (‑500/‑66) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 120780566 (0.630)35 (0.350) 25/556 2.7 35.8% coding (305/630 nt) ycfK protein StfP
?NC_000913 = 1209602 63 (0.640)intergenic (‑483/‑83) tfaE/pinE putative tail fiber assembly protein TfaE/site‑specific DNA recombinase
* ? NC_000913 = 1397238NA (NA)90 (0.860) 60/588 0.3 100% noncoding (1195/1195 nt) IS5 repeat region
?NC_000913 = 1978494 0 (0.000)intergenic (‑297/+24) flhD/insB5 DNA‑binding transcriptional dual regulator FlhD/IS1 family protein InsB
* ? NC_000913 1979271 =0 (0.000)129 (1.230) 66/588 0.2 100% intergenic (‑56/‑482) insA5/uspC IS1 family protein InsA/universal stress protein C
?NC_000913 = 2102947 NA (NA)pseudogene (443/446 nt) wbbL interrupted rhamnosyltransferase WbbL