Predicted mutation
evidence seq id position mutation annotation gene description
RA NC_000913 4,296,381 +GC intergenic (+587/+55) gltP → / ← yjcO glutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NC_0009134,296,3801.C96.4% 280.0 / 4.5 86intergenic (+586/+56)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base C (47/34);  total (50/36)
Fisher's exact test for biased strand distribution p-value = 2.69e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00
*NC_0009134,296,3802.G96.5% 294.4 / 1.9 86intergenic (+586/+56)gltP/yjcOglutamate/aspartate : H(+) symporter GltP/Sel1 repeat‑containing protein YjcO
Reads supporting (aligned to +/- strand):  ref base . (3/0);  new base G (47/36);  total (50/36)
Fisher's exact test for biased strand distribution p-value = 2.61e-01
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00

TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGT  >  NC_000913/4296139‑4296665
                                                                                                                                                                                                                                                       ||                                                                                                                                                                                                                                                                                                      
tCGGATAGGGTGTTTATGCCTCATCCGGCGTCAACGCCTGATGCGACGCTTGCCGCGT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCt                                                                                                                                                                                                                                            <  2:668986/300‑1 (MQ=11)
     taagacgtggactccGCACCCGACATCAACGCCTGATTCGACGCTTG‑CTCTTTCTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAcc                                                                                                                                                                                                                                       <  2:172416/287‑1 (MQ=11)
                       tCCGACATCAACGCCTGATGCGACGCTTGCCGCGT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑CGNC‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaa‑‑a                                                                                                                                                                                                                   >  1:179897/1‑300 (MQ=255)
                         cGACATCAACGCCTGATGCGACGCTTGCCGCGT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCaacct                                                                                                                                                                                                                   >  2:19457/1‑297 (MQ=255)
                                aaCGCCTGATGCGACGCTTGCTGCGT‑CTTATCATGCCTACGCCAGACAGCGCG‑A‑TAGCCTGATTTCGTGTGGTTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑Tcaca                                                                                                                                                                                                        <  2:88173/300‑1 (MQ=14)
                                  cGCCTGATGCGACGCTTGCCGCGT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACAcc                                                                                                                                                                                                      >  1:465444/1‑300 (MQ=255)
                                  cGCCTGATGCGACGCTTGCCGCGT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAAGCGTTCATGCCGCATCCGACACCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATGGCCTGATTTAGCGTGGTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTCTCAGGCCTATCTTCACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACAcc                                                                                                                                                                                                      >  1:467289/1‑300 (MQ=11)
                                    ccTGATGCGACGCTTGCCGCGT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAAACAGCGCAACATCCTGATCTAGCGTAACCTTGTAGGTCGAATACGGCACTT‑‑C‑‑CG‑CTGCA‑TCCGAAATCACTGCCTGCTGTGCAGTTTGCCGCGTCTCATCAGGCCTATCTTAAACGTTGGTCAATTTTTCAA‑‑ACTCT‑‑Tcac                                                                                                                                                                                                         >  2:522954/1‑295 (MQ=11)
                                                       cgT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                 >  1:762050/1‑300 (MQ=255)
                                                        gT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCATTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                 <  1:35225/299‑1 (MQ=255)
                                                        gT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                 <  1:35235/299‑1 (MQ=255)
                                                         t‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                 <  1:127353/298‑1 (MQ=255)
                                                             tATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTc                                                                                                                                                                            >  1:762133/1‑300 (MQ=255)
                                                             tATCAGCCCTACGCCAGACAGCTCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGTGTTTAGGCCGCATTCGGCATCAACGCCTGATGCGACGCTGAACGCGTCTTATCAGCCCTACGCCAGACAGCGCAAGAGCCTGATTTAGCGTGATTTTTTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTc                                                                                                                                                                            <  2:297829/300‑1 (MQ=17)
                                                                  ggCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGtt                                                                                                                                                                       >  1:267051/1‑300 (MQ=255)
                                                                   gCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTc                                                                                                                                                                      >  1:664055/1‑300 (MQ=255)
                                                                       aCGCCAGACAGCGCAGNGTAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCACTCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTCTCTTACCCGTTGGTTAATTTTTCAGGGACTCTCNTCACACCCGGTATCaaggncccttccctaccggtct                                                                                                                                                                         >  1:689143/1‑280 (MQ=11)
                                                                       aCGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATTAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTACATACAGCTCAGGTTCaa                                                                                                                                                                    >  2:710290/1‑298 (MQ=255)
                                                                       aCGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACGGCGCAATATTCTGATTTATCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TTTGAAATCACTGCCTGCTGTGAAGCTTGCCGCGTCTTATAAGGCCTATCTTAAATGTGGGTTAAttttt                                                                                                                                                                                                                         >  2:731595/1‑249 (MQ=11)
                                                                       aCGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTNATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAc                                                                                                                                                                   >  2:609336/1‑299 (MQ=255)
                                                                       aCGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAAcc                                                                                                                                                                  >  1:194863/1‑300 (MQ=255)
                                                                       aCGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCAATACAGCTCAGGTTCAAcc                                                                                                                                                                  >  1:263134/1‑300 (MQ=255)
                                                                       aCGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTTAAcc                                                                                                                                                                  >  1:245512/1‑300 (MQ=255)
                                                                       aCGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GAA‑TCCGACATCAATGCCTGATGCGAAGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCaagcc                                                                                                                                                                                          >  2:334818/1‑273 (MQ=255)
                                                                                 gcgcA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgcgc                                                                                                                                                        <  1:49976/300‑1 (MQ=255)
                                                                                 gcgcA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGAAATCAATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTACATACAGCTCAGGTTCAACTAGTGCAgcgc                                                                                                                                                        >  2:344157/1‑300 (MQ=255)
                                                                                 gcgcA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGAAATCAATGCATGATGCGACGCTTGCCGCGTCTCATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACCCCCGGTATCAGACTCTTCCAt                                                                                                                                                                                   >  2:494933/1‑273 (MQ=14)
                                                                                 gcgcA‑A‑TAGCCTGATTTAGAGTGATTTGGGAGGTCGGAGAGGGCGTTGATGCCGCATCCGACATCAACGCCGGATGCGACGCGTAACGCGTCTTATCAGACCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGAGTTTGTAGTGCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgcgc                                                                                                                                                        <  2:463915/300‑1 (MQ=18)
                                                                                   gcA‑A‑TATCCTGATTTAGCTTGATTTTGTAGTTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCACGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                 <  2:317472/273‑1 (MQ=17)
                                                                                         tAGCCTGATTGCGTGTGATTTTGTAGTTCGGATAGGGCGTTTATGCCACATCCGACATCAACGCCTGATTCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCTTGATTTTGTAGTTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAAc                                                                                                                                                                                           <  2:68250/259‑1 (MQ=17)
                                                                                           gCCTGGTTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGTGACGCTTAACGCGTCTTATCAAGCCTACGCCAGACAGTGCAATAGTCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCCATGCCTGATGCGACGCTTGCAGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAA‑‑ACTCT‑‑Tcac                                                                                                                                                                                                         >  2:437052/1‑243 (MQ=14)
                                                                                           gCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCt                                                                                                                                                >  1:227859/1‑300 (MQ=255)
                                                                                               gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTCCG‑‑C‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGtt                                                                                                                                            >  1:57175/1‑300 (MQ=255)
                                                                                                                  ggTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCATCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGTCTTTTGCTTGTTCTTCTCGATAAAACcttct                                                                                                                         >  1:730140/1‑300 (MQ=255)
                                                                                                                       gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATCCAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTAGATAAAACCTTCTTCAcc                                                                                                                    >  1:106369/1‑300 (MQ=255)
                                                                                                                       gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCttattccac                                                                                                                    >  2:155383/1‑297 (MQ=255)
                                                                                                                       gATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCAcc                                                                                                                    >  1:106529/1‑300 (MQ=255)
                                                                                                                            ggCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTa                                                                                                               >  1:785148/1‑300 (MQ=255)
                                                                                                                               gTTTATGCCGCATTCGACAT‑AACGCCTGATGCGACGCTTAACGTGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAg                                                                                                             <  1:1321/298‑1 (MQ=255)
                                                                                                                                 ttATGCCACATAGGACATCAACGCTGGAGGCGACGCTTAACGCGTCTTATTAGGCAGACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGaa                                                                                                           <  2:524471/299‑1 (MQ=25)
                                                                                                                                              ggcgtCAACGCCTGATGCGACGCTTACCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                 <  2:421679/212‑1 (MQ=255)
                                                                                                                                              gatgtCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ATGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGccc                                                                                             <  1:435818/296‑1 (MQ=255)
                                                                                                                                                 gtCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTGAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCG‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAgcgc                                                                                                                                                        <  2:141642/237‑1 (MQ=18)
                                                                                                                                                 gtCAACGCCTGATGCGACGCTTGACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATAc                                                                                                                                                                                 <  2:421600/212‑1 (MQ=255)
                                                                                                                                                 aTCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACACTTCCATACAGCTCAGGTTCAAACAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGt                                                                                          >  1:830257/1‑300 (MQ=255)
                                                                                                                                                    aactccTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGTTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTAc                                                                                        <  2:773563/295‑1 (MQ=255)
                                                                                                                                                      cGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCATATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCTGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTATCGATAAAACCTTCTTCACCGTTTAAGAACATCACTCCCGCCCAGTACTc                                                                                      >  1:65209/1‑299 (MQ=255)
                                                                                                                                                       gCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTc                                                                                      >  2:670033/1‑298 (MQ=255)
                                                                                                                                                          tGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGa                                                                                   <  1:454551/298‑1 (MQ=255)
                                                                                                                                                              gcggcgatgaaCGCGTCGGATAAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCTTTGGTTAATGTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAcc                                                                             <  1:459942/291‑1 (MQ=37)
                                                                                                                                                              gCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAAcc                                                                             <  1:458274/300‑1 (MQ=255)
                                                                                                                                                                 aCGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCg                                                                            <  2:84337/298‑1 (MQ=255)
                                                                                                                                                                 aCGCTTAACGCGTCTTATCAGACCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAATGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTAc                                                                                        <  2:332682/286‑1 (MQ=255)
                                                                                                                                                                                tATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCa‑ga                                                          >  1:410414/1‑300 (MQ=255)
                                                                                                                                                                                    aGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTg                                                      <  2:521355/300‑1 (MQ=255)
                                                                                                                                                                                     ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGc                                                     >  1:395889/1‑300 (MQ=255)
                                                                                                                                                                                     ggCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCATTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTACATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGc                                                     >  1:631496/1‑300 (MQ=255)
                                                                                                                                                                                         tACGCCAGACAGCGCAATAGCCGGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTg                                                      <  2:521432/295‑1 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGGTTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAANCCTTCTTCACCGTTTAAGAACCTCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGttt                                                 >  1:178884/1‑299 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAANCCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGttt                                                 >  1:156561/1‑299 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTATTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGttt                                                 >  1:338076/1‑299 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGttt                                                 >  1:27855/1‑299 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTAGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGttt                                                 >  2:65490/1‑299 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCACAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGttt                                                 >  1:338236/1‑299 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTACATATAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACCCGGAATAACCGGTCCGGGAAATTGCA‑GAGCTGCGttt                                                 >  2:431848/1‑299 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCATTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGttt                                                 >  1:745755/1‑299 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAAACGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTATTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACTGGTTCGGGAAATTGCA‑GAGCTGCGttt                                                 >  2:169725/1‑299 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCCTGATGTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGTCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGttt                                                 <  1:305662/299‑1 (MQ=255)
                                                                                                                                                                                          aCGCCAGACAGCGCAATAGCATGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTCAGAACATCATTCACGCCCAGTACTCGGAATAACCGGTTCGGGGAATTGCA‑GAGCTGCGttt                                                 >  2:235105/1‑299 (MQ=255)
                                                                                                                                                                                               aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATa                                           >  1:487860/1‑300 (MQ=255)
                                                                                                                                                                                               aGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAACGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCCC‑‑CCTCT‑‑TCACCCCCGGTATCAAACCCTTCCATCCCGCTCCAGTTCAACCCGTGCAACGACTTTTGCTTTTTCTTCTCTATCCAACCTTCTTCCCCGTTTCAGACCATCCTTCCCGCCCAGTACTCGGAATACCCGGTTCGGGAAATTTCA‑GAGCTTCGCTTGCAATa                                           >  1:807833/1‑300 (MQ=18)
                                                                                                                                                                                                    gcgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTAGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCa                                        >  2:88946/1‑298 (MQ=255)
                                                                                                                                                                                                     cgcAATAGCCTTATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGTTTGCCGCGTCTTATCAGGCCTATCGTAACCGTTGGTTAATGTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATANAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCNTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATAcc                                         <  2:636728/296‑1 (MQ=255)
                                                                                                                                                                                                     cgcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ATGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCg                                                                            <  2:84299/262‑1 (MQ=255)
                                                                                                                                                                                                      gcAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGt                                     <  1:221524/299‑1 (MQ=255)
                                                                                                                                                                                                               tGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCa                           <  1:670033/300‑1 (MQ=255)
                                                                                                                                                                                                                gATTTAGCGTGATTTTGTAGGTCGGATAGCCCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTCCAACCAGTGCAGCGCCCTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCCCGAGCTGCGTTTGAAATACCAGGTTGCCTTGTCa                           <  2:689143/300‑1 (MQ=38)
                                                                                                                                                                                                                gATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCAt                          >  1:816547/1‑300 (MQ=255)
                                                                                                                                                                                                                    tAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCAtcgtc                      <  1:19457/300‑1 (MQ=255)
                                                                                                                                                                                                                        gTGATTTTGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGcc                  <  2:709580/300‑1 (MQ=255)
                                                                                                                                                                                                                              ttttAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATg            <  2:581193/297‑1 (MQ=255)
                                                                                                                                                                                                                              ttGTAGGTCGGATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATg            <  1:88946/300‑1 (MQ=255)
                                                                                                                                                                                                                                gTAGGTCGGATAATGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTNCTTGTTCTTCTCGATAAAACCTTCTTCACCGTNTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGcc          <  2:618607/300‑1 (MQ=255)
                                                                                                                                                                                                                                       ggATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGcc    >  1:677117/1‑299 (MQ=255)
                                                                                                                                                                                                                                        gATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGt  >  2:287386/1‑300 (MQ=255)
                                                                                                                                                                                                                                        gATAAGGCGTTT‑‑ACGC‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGt  >  1:751497/1‑300 (MQ=255)
                                                                                                                                                                                                                                                       ||                                                                                                                                                                                                                                                                                                      
TCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGT‑CTTATCAGGCCTACGCCAGACAGCGCA‑A‑TAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTTATGCCGCATCCGACATCAACGCCTGATGCGACGCTTAACGCGTCTTATCAGGCCTACGCCAGACAGCGCAATAGCCTGATTTAGCGTGATTTTGTAGGTCGGATAAGGCGTTT‑‑A‑‑C‑‑C‑GCA‑TCCGACATCAATGCCTGATGCGACGCTTGCCGCGTCTTATCAGGCCTATCTTAACCGTTGGTTAATTTTTCAA‑‑ACTCT‑‑TCACACCCGGTATCAAACCCTTCCATACAGCTCAGGTTCAACCAGTGCAGCGCCTTTTGCTTGTTCTTCTCGATAAAACCTTCTTCACCGTTTAAGAACATCATTCCCGCCCAGTACTCGGAATAACCGGTTCGGGAAATTGCA‑GAGCTGCGTTTGAAATACCAGGTTGCCTTGTCATCGTCGGCCTTAATGCCCACGCCGT  >  NC_000913/4296139‑4296665

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 40 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑